Genotoxicity following organophosphate pesticides exposure among Orang Asli children living in an agricultural island in Kuala Langat, Selangor, Malaysia
Background: Agriculture is an important sector for the Malaysian economy. The use of pesticides in agriculture is crucial due to its function in keeping the crops from harmful insects. Children living near agricultural fields are at risk of pesticide poisoning. Objective: To evaluate the genotoxic r...
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| Format: | Article |
| Language: | English |
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National Iranian Oil Company (N I O C) Polyclinics
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54381/ http://psasir.upm.edu.my/id/eprint/54381/1/Genotoxicity%20following%20organophosphate%20pesticides%20exposure%20.pdf |
| _version_ | 1848852529775902720 |
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| author | Mat Sutris, Juliana How, Vivien Sumeri, Siti Arfah Muhammad, M. Sardi Danori, Mohd Danori Mohd Mokhtar, M. T. Muhammad, H. Ghazi, Hasanain Faisal Md Isa, Zaleha |
| author_facet | Mat Sutris, Juliana How, Vivien Sumeri, Siti Arfah Muhammad, M. Sardi Danori, Mohd Danori Mohd Mokhtar, M. T. Muhammad, H. Ghazi, Hasanain Faisal Md Isa, Zaleha |
| author_sort | Mat Sutris, Juliana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Agriculture is an important sector for the Malaysian economy. The use of pesticides in agriculture is crucial due to its function in keeping the crops from harmful insects. Children living near agricultural fields are at risk of pesticide poisoning. Objective: To evaluate the genotoxic risk among children who exposed to pesticides and measure DNA damage due to pesticides exposure. Methods: In a cross-sectional study 180 Orang Asli Mah Meri children aged between 7 and 12 years were studied. They were all living in an agricultural island in Kuala Langat, Selangor, Malaysia. The data for this study were collected via modified validated questionnaire and food frequency questionnaire, which consisted of 131 food items. 6 urinary organophosphate metabolites were used as biomarkers for pesticides exposure. For genotoxic risk or genetic damage assessment, the level of DNA damage from exfoliated buccal mucosa cells was measured using the comet assay electrophoresis method. Results: Out of 180 respondents, 84 (46.7%) showed positive traces of organophosphate metabolites in their urine. Children with detectable urinary pesticide had a longer tail length (median 43.5; IQR 30.9 to 68.1 μm) than those with undetectable urinary pesticides (median 24.7; IQR 9.5 to 48.1 μm). There was a significant association between the extent of DNA damage and the children's age, length of residence in the area, pesticides detection, and frequency of apple consumption. Conclusion: The organophosphate genotoxicity among children is associated with the amount of exposure (detectability of urinary pesticide) and length of residence in (exposure) the study area. |
| first_indexed | 2025-11-15T10:39:32Z |
| format | Article |
| id | upm-54381 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:39:32Z |
| publishDate | 2016 |
| publisher | National Iranian Oil Company (N I O C) Polyclinics |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-543812018-03-14T09:17:23Z http://psasir.upm.edu.my/id/eprint/54381/ Genotoxicity following organophosphate pesticides exposure among Orang Asli children living in an agricultural island in Kuala Langat, Selangor, Malaysia Mat Sutris, Juliana How, Vivien Sumeri, Siti Arfah Muhammad, M. Sardi Danori, Mohd Danori Mohd Mokhtar, M. T. Muhammad, H. Ghazi, Hasanain Faisal Md Isa, Zaleha Background: Agriculture is an important sector for the Malaysian economy. The use of pesticides in agriculture is crucial due to its function in keeping the crops from harmful insects. Children living near agricultural fields are at risk of pesticide poisoning. Objective: To evaluate the genotoxic risk among children who exposed to pesticides and measure DNA damage due to pesticides exposure. Methods: In a cross-sectional study 180 Orang Asli Mah Meri children aged between 7 and 12 years were studied. They were all living in an agricultural island in Kuala Langat, Selangor, Malaysia. The data for this study were collected via modified validated questionnaire and food frequency questionnaire, which consisted of 131 food items. 6 urinary organophosphate metabolites were used as biomarkers for pesticides exposure. For genotoxic risk or genetic damage assessment, the level of DNA damage from exfoliated buccal mucosa cells was measured using the comet assay electrophoresis method. Results: Out of 180 respondents, 84 (46.7%) showed positive traces of organophosphate metabolites in their urine. Children with detectable urinary pesticide had a longer tail length (median 43.5; IQR 30.9 to 68.1 μm) than those with undetectable urinary pesticides (median 24.7; IQR 9.5 to 48.1 μm). There was a significant association between the extent of DNA damage and the children's age, length of residence in the area, pesticides detection, and frequency of apple consumption. Conclusion: The organophosphate genotoxicity among children is associated with the amount of exposure (detectability of urinary pesticide) and length of residence in (exposure) the study area. National Iranian Oil Company (N I O C) Polyclinics 2016-01 Article NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/54381/1/Genotoxicity%20following%20organophosphate%20pesticides%20exposure%20.pdf Mat Sutris, Juliana and How, Vivien and Sumeri, Siti Arfah and Muhammad, M. and Sardi Danori, Mohd Danori and Mohd Mokhtar, M. T. and Muhammad, H. and Ghazi, Hasanain Faisal and Md Isa, Zaleha (2016) Genotoxicity following organophosphate pesticides exposure among Orang Asli children living in an agricultural island in Kuala Langat, Selangor, Malaysia. The International Journal of Occupational and Environmental Medicine, 7 (1). pp. 42-51. ISSN 2008-6520; ESSN: 2008-6814 http://www.theijoem.com/ijoem/index.php/ijoem/article/view/705 Organophosphates; Pesticides; Organophosphate poisoning; Mutagenicity tests; Comet assay, Child; Malaysia 10.15171/ijoem.2016.705 |
| spellingShingle | Organophosphates; Pesticides; Organophosphate poisoning; Mutagenicity tests; Comet assay, Child; Malaysia Mat Sutris, Juliana How, Vivien Sumeri, Siti Arfah Muhammad, M. Sardi Danori, Mohd Danori Mohd Mokhtar, M. T. Muhammad, H. Ghazi, Hasanain Faisal Md Isa, Zaleha Genotoxicity following organophosphate pesticides exposure among Orang Asli children living in an agricultural island in Kuala Langat, Selangor, Malaysia |
| title | Genotoxicity following organophosphate pesticides exposure among Orang Asli children living in an agricultural island in Kuala Langat, Selangor, Malaysia |
| title_full | Genotoxicity following organophosphate pesticides exposure among Orang Asli children living in an agricultural island in Kuala Langat, Selangor, Malaysia |
| title_fullStr | Genotoxicity following organophosphate pesticides exposure among Orang Asli children living in an agricultural island in Kuala Langat, Selangor, Malaysia |
| title_full_unstemmed | Genotoxicity following organophosphate pesticides exposure among Orang Asli children living in an agricultural island in Kuala Langat, Selangor, Malaysia |
| title_short | Genotoxicity following organophosphate pesticides exposure among Orang Asli children living in an agricultural island in Kuala Langat, Selangor, Malaysia |
| title_sort | genotoxicity following organophosphate pesticides exposure among orang asli children living in an agricultural island in kuala langat, selangor, malaysia |
| topic | Organophosphates; Pesticides; Organophosphate poisoning; Mutagenicity tests; Comet assay, Child; Malaysia |
| url | http://psasir.upm.edu.my/id/eprint/54381/ http://psasir.upm.edu.my/id/eprint/54381/ http://psasir.upm.edu.my/id/eprint/54381/ http://psasir.upm.edu.my/id/eprint/54381/1/Genotoxicity%20following%20organophosphate%20pesticides%20exposure%20.pdf |