Fluorometric immunoassay for detecting the plant virus Citrus tristeza using carbon nanoparticles acting as quenchers and antibodies labeled with CdTe quantum dots
Cadmium-telluride quantum dots (QDs) were conjugated to an antibody (Ab) against Citrus tristeza virus (CTV), while the coat protein (CP) of the CTV was immobilized on the surface of carbon nanoparticles (CNPs). Following immunobinding of the QD-Ab and the CP-loaded CNPs, the fluorescence of the CdT...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Verlag
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54334/ http://psasir.upm.edu.my/id/eprint/54334/1/Fluorometric%20immunoassay%20for%20detecting%20the%20plant%20virus%20Citrus%20tristeza%20.pdf |
| _version_ | 1848852516566990848 |
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| author | Shojaei, Taha Roodbar Mohd Salleh, Mohamad Amran Sijam, Kamaruzaman Abdul Rahim, Raha Mohsenifar, Afshin Safarnejad, Reza Tabatabaei, Meisam |
| author_facet | Shojaei, Taha Roodbar Mohd Salleh, Mohamad Amran Sijam, Kamaruzaman Abdul Rahim, Raha Mohsenifar, Afshin Safarnejad, Reza Tabatabaei, Meisam |
| author_sort | Shojaei, Taha Roodbar |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Cadmium-telluride quantum dots (QDs) were conjugated to an antibody (Ab) against Citrus tristeza virus (CTV), while the coat protein (CP) of the CTV was immobilized on the surface of carbon nanoparticles (CNPs). Following immunobinding of the QD-Ab and the CP-loaded CNPs, the fluorescence of the CdTe QDs was quenched by the CNPs. This effect was exploited to design a detection assay for the CTV which was found more sensitive and specific than the existing enzyme linked immunosorbent assay (ELISA). The limit of detection was measured at about 220 ng⋅ mL‾1 of CTV. The Stern-Volmer plot of the CNPs-QD quencher pair showed a positive deviation from linearity which was ascribed to the presence of both static and dynamic quenching. |
| first_indexed | 2025-11-15T10:39:19Z |
| format | Article |
| id | upm-54334 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:39:19Z |
| publishDate | 2016 |
| publisher | Springer Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-543342018-03-14T01:11:12Z http://psasir.upm.edu.my/id/eprint/54334/ Fluorometric immunoassay for detecting the plant virus Citrus tristeza using carbon nanoparticles acting as quenchers and antibodies labeled with CdTe quantum dots Shojaei, Taha Roodbar Mohd Salleh, Mohamad Amran Sijam, Kamaruzaman Abdul Rahim, Raha Mohsenifar, Afshin Safarnejad, Reza Tabatabaei, Meisam Cadmium-telluride quantum dots (QDs) were conjugated to an antibody (Ab) against Citrus tristeza virus (CTV), while the coat protein (CP) of the CTV was immobilized on the surface of carbon nanoparticles (CNPs). Following immunobinding of the QD-Ab and the CP-loaded CNPs, the fluorescence of the CdTe QDs was quenched by the CNPs. This effect was exploited to design a detection assay for the CTV which was found more sensitive and specific than the existing enzyme linked immunosorbent assay (ELISA). The limit of detection was measured at about 220 ng⋅ mL‾1 of CTV. The Stern-Volmer plot of the CNPs-QD quencher pair showed a positive deviation from linearity which was ascribed to the presence of both static and dynamic quenching. Springer Verlag 2016-07 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54334/1/Fluorometric%20immunoassay%20for%20detecting%20the%20plant%20virus%20Citrus%20tristeza%20.pdf Shojaei, Taha Roodbar and Mohd Salleh, Mohamad Amran and Sijam, Kamaruzaman and Abdul Rahim, Raha and Mohsenifar, Afshin and Safarnejad, Reza and Tabatabaei, Meisam (2016) Fluorometric immunoassay for detecting the plant virus Citrus tristeza using carbon nanoparticles acting as quenchers and antibodies labeled with CdTe quantum dots. Microchimica Acta, 183 (7/July). pp. 2277-2287. ISSN 0026-3672; ESSN:1436-5073 https://link.springer.com/article/10.1007/s00604-016-1867-7 Bioassay; Competitive quenching; Stern-Volmer relationship; ELISA; TEM; FTIR; CdTe; Synthesis; Bioconjugation 10.1007/s00604-016-1867-7 |
| spellingShingle | Bioassay; Competitive quenching; Stern-Volmer relationship; ELISA; TEM; FTIR; CdTe; Synthesis; Bioconjugation Shojaei, Taha Roodbar Mohd Salleh, Mohamad Amran Sijam, Kamaruzaman Abdul Rahim, Raha Mohsenifar, Afshin Safarnejad, Reza Tabatabaei, Meisam Fluorometric immunoassay for detecting the plant virus Citrus tristeza using carbon nanoparticles acting as quenchers and antibodies labeled with CdTe quantum dots |
| title | Fluorometric immunoassay for detecting the plant virus Citrus tristeza using carbon nanoparticles acting as quenchers and antibodies labeled with CdTe quantum dots |
| title_full | Fluorometric immunoassay for detecting the plant virus Citrus tristeza using carbon nanoparticles acting as quenchers and antibodies labeled with CdTe quantum dots |
| title_fullStr | Fluorometric immunoassay for detecting the plant virus Citrus tristeza using carbon nanoparticles acting as quenchers and antibodies labeled with CdTe quantum dots |
| title_full_unstemmed | Fluorometric immunoassay for detecting the plant virus Citrus tristeza using carbon nanoparticles acting as quenchers and antibodies labeled with CdTe quantum dots |
| title_short | Fluorometric immunoassay for detecting the plant virus Citrus tristeza using carbon nanoparticles acting as quenchers and antibodies labeled with CdTe quantum dots |
| title_sort | fluorometric immunoassay for detecting the plant virus citrus tristeza using carbon nanoparticles acting as quenchers and antibodies labeled with cdte quantum dots |
| topic | Bioassay; Competitive quenching; Stern-Volmer relationship; ELISA; TEM; FTIR; CdTe; Synthesis; Bioconjugation |
| url | http://psasir.upm.edu.my/id/eprint/54334/ http://psasir.upm.edu.my/id/eprint/54334/ http://psasir.upm.edu.my/id/eprint/54334/ http://psasir.upm.edu.my/id/eprint/54334/1/Fluorometric%20immunoassay%20for%20detecting%20the%20plant%20virus%20Citrus%20tristeza%20.pdf |