Facile modulation of enantioselectivity of thermophilic Geobacillus zalihae lipase by regulating hydrophobicity of its Q114 oxyanion

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Facile modulation of enantioselectivity of thermophilic Geobacillus zalihae lipase by regulating hydrophobicity of its Q114 oxyanion

Site-directed mutagenesis of the oxyanion-containing amino acid Q114 in the recombinant thermophilic T1 lipase previously isolated from Geobacillus zalihae was performed to elucidate its role in the enzyme⿿s enantioselectivity and reactivity. Substitution of Q114 with a hydrophobic methionine to yie...

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Bibliographic Details
Main Authors:	Abdul Wahab, Roswanira, Basri, Mahiran, Raja Abdul Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Abdul Rahman, Mohd Basyaruddin, Leow, Adam Thean Chor
Format:	Article
Language:	English
Published:	Elsevier 2016
Subjects:	T1 lipase; Geobacillus zalihae; 2DSN; Site-directed mutagenesis; Enantioselectivity; Oxyanion
Online Access:	http://psasir.upm.edu.my/id/eprint/54264/ http://psasir.upm.edu.my/id/eprint/54264/1/Facile%20modulation%20of%20enantioselectivity%20of%20thermophilic%20Geobacillus%20zalihae%20lipase%20by%20regulating%20hydrophobicity%20of%20its%20Q114%20oxyanion.pdf

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