Fabrication of graphene/gold-modified screen-printed electrode for detection of carcinoembryonic antigen
Immunosensors based on gold nanoparticles and reduced graphene oxide (AuNPs/rGO)-modified screen-printed electrodes (SPEs) were successfully synthesized using an electrochemical deposition method. The modified SPEs were characterized using a field emission scanning electron microscope (FESEM) and Ra...
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| Format: | Article |
| Language: | English |
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Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54259/ http://psasir.upm.edu.my/id/eprint/54259/1/Fabrication%20of%20graphene.pdf |
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| author | Chan, K. F. Lim, Hong Ngee Shams, Nafiseh Jayabal, Subramaniam Pandikumar, Alagarsamy Huang, Nay Ming |
| author_facet | Chan, K. F. Lim, Hong Ngee Shams, Nafiseh Jayabal, Subramaniam Pandikumar, Alagarsamy Huang, Nay Ming |
| author_sort | Chan, K. F. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Immunosensors based on gold nanoparticles and reduced graphene oxide (AuNPs/rGO)-modified screen-printed electrodes (SPEs) were successfully synthesized using an electrochemical deposition method. The modified SPEs were characterized using a field emission scanning electron microscope (FESEM) and Raman spectroscopy to analyze the morphology and composition of AuNPs and rGO. Both the FESEM and Raman spectroscopy revealed that the AuNPs were successfully anchored on the thin film of rGO deposited on the surface of the SPEs. Characterization with a ferri–ferrocyanide couple [Fe(CN)63 −/4 −] showed that the electron transfer kinetic between the analyte and electrode was enhanced after the modification with the AuNPs/rGO composite on the electrode surface, in addition to increasing the effective surface area of the electrode. The modified SPE was immobilized with a sandwich type immunosensor to mimic the ELISA (enzyme-linked immunosorbent assay) immunoassay. The modified SPE that was fortified with the sandwich type immunosensor exhibited double electrochemical responses in the detection of carcinoembryonic antigen (CEA), with linear ranges of 0.5–50 ng/mL and 250–2000 ng/mL and limits of detection of 0.28 ng/mL and 181.5 ng/mL, respectively. |
| first_indexed | 2025-11-15T10:39:00Z |
| format | Article |
| id | upm-54259 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:39:00Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-542592018-03-09T02:13:27Z http://psasir.upm.edu.my/id/eprint/54259/ Fabrication of graphene/gold-modified screen-printed electrode for detection of carcinoembryonic antigen Chan, K. F. Lim, Hong Ngee Shams, Nafiseh Jayabal, Subramaniam Pandikumar, Alagarsamy Huang, Nay Ming Immunosensors based on gold nanoparticles and reduced graphene oxide (AuNPs/rGO)-modified screen-printed electrodes (SPEs) were successfully synthesized using an electrochemical deposition method. The modified SPEs were characterized using a field emission scanning electron microscope (FESEM) and Raman spectroscopy to analyze the morphology and composition of AuNPs and rGO. Both the FESEM and Raman spectroscopy revealed that the AuNPs were successfully anchored on the thin film of rGO deposited on the surface of the SPEs. Characterization with a ferri–ferrocyanide couple [Fe(CN)63 −/4 −] showed that the electron transfer kinetic between the analyte and electrode was enhanced after the modification with the AuNPs/rGO composite on the electrode surface, in addition to increasing the effective surface area of the electrode. The modified SPE was immobilized with a sandwich type immunosensor to mimic the ELISA (enzyme-linked immunosorbent assay) immunoassay. The modified SPE that was fortified with the sandwich type immunosensor exhibited double electrochemical responses in the detection of carcinoembryonic antigen (CEA), with linear ranges of 0.5–50 ng/mL and 250–2000 ng/mL and limits of detection of 0.28 ng/mL and 181.5 ng/mL, respectively. Elsevier 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54259/1/Fabrication%20of%20graphene.pdf Chan, K. F. and Lim, Hong Ngee and Shams, Nafiseh and Jayabal, Subramaniam and Pandikumar, Alagarsamy and Huang, Nay Ming (2016) Fabrication of graphene/gold-modified screen-printed electrode for detection of carcinoembryonic antigen. Materials Science & Engineering C-Materials for Biological Applications, 58. pp. 666-674. ISSN 0928-4931 https://www.sciencedirect.com/science/article/pii/S0928493115303349 Biosensor; Electrochemistry; Gold nanoparticles; Graphene; Screen-printed electrode 10.1016/j.msec.2015.09.010 |
| spellingShingle | Biosensor; Electrochemistry; Gold nanoparticles; Graphene; Screen-printed electrode Chan, K. F. Lim, Hong Ngee Shams, Nafiseh Jayabal, Subramaniam Pandikumar, Alagarsamy Huang, Nay Ming Fabrication of graphene/gold-modified screen-printed electrode for detection of carcinoembryonic antigen |
| title | Fabrication of graphene/gold-modified screen-printed electrode for detection of carcinoembryonic antigen |
| title_full | Fabrication of graphene/gold-modified screen-printed electrode for detection of carcinoembryonic antigen |
| title_fullStr | Fabrication of graphene/gold-modified screen-printed electrode for detection of carcinoembryonic antigen |
| title_full_unstemmed | Fabrication of graphene/gold-modified screen-printed electrode for detection of carcinoembryonic antigen |
| title_short | Fabrication of graphene/gold-modified screen-printed electrode for detection of carcinoembryonic antigen |
| title_sort | fabrication of graphene/gold-modified screen-printed electrode for detection of carcinoembryonic antigen |
| topic | Biosensor; Electrochemistry; Gold nanoparticles; Graphene; Screen-printed electrode |
| url | http://psasir.upm.edu.my/id/eprint/54259/ http://psasir.upm.edu.my/id/eprint/54259/ http://psasir.upm.edu.my/id/eprint/54259/ http://psasir.upm.edu.my/id/eprint/54259/1/Fabrication%20of%20graphene.pdf |