Extraction and characterization of oligosaccharides from palm kernel cake as prebiotic

The main objective of the present study was to extract and characterize oligosaccharides from palm kernel cake (OligoPKC) to be used as a prebiotic. Up to 16.81% of oligosaccharides were extracted from PKC using neutral detergent solution with two to eight degrees of polymerization. Molecular weight...

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Main Authors: Jahromi, Mohammad Faseleh, Liang, Juan Boo, Abdullah, Norhani, Goh, Yong Meng, Ebrahimi, Rohollah, Shokryazdan, Parisa
Format: Article
Language:English
Published: North Carolina State University 2016
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/54254/
http://psasir.upm.edu.my/id/eprint/54254/1/Extraction%20and%20characterization%20of%20oligosaccharides%20from%20palm%20kernel%20cake%20as%20prebiotic.pdf
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author Jahromi, Mohammad Faseleh
Liang, Juan Boo
Abdullah, Norhani
Goh, Yong Meng
Ebrahimi, Rohollah
Shokryazdan, Parisa
author_facet Jahromi, Mohammad Faseleh
Liang, Juan Boo
Abdullah, Norhani
Goh, Yong Meng
Ebrahimi, Rohollah
Shokryazdan, Parisa
author_sort Jahromi, Mohammad Faseleh
building UPM Institutional Repository
collection Online Access
description The main objective of the present study was to extract and characterize oligosaccharides from palm kernel cake (OligoPKC) to be used as a prebiotic. Up to 16.81% of oligosaccharides were extracted from PKC using neutral detergent solution with two to eight degrees of polymerization. Molecular weights of seven fractions of OligoPKC were estimated using a mass spectrophotometer procedure resembling those of mannobiose, mannotriose, mannotetraose, mannopentaose, and mannohexaose standards, while those of two unknown components resembled those of heptasaccharide and octasaccharide. Enzymatic hydrolysis of OligoPKC using 11 enzymes showed that β-mannosidase and β-mannanase had the highest effects. OligoPKC fractions were potential substrates for growth of four species of Lactobacillus. Supplementation of OligoPKC in the diet of broiler chickens increased the population of beneficial microbes. However, it reduced the populations of pathogenic bacteria in the cecum. Hence, OligoPKC can be considered a potential prebiotic supplement in the feed and food industry.
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spelling upm-542542018-03-08T09:02:16Z http://psasir.upm.edu.my/id/eprint/54254/ Extraction and characterization of oligosaccharides from palm kernel cake as prebiotic Jahromi, Mohammad Faseleh Liang, Juan Boo Abdullah, Norhani Goh, Yong Meng Ebrahimi, Rohollah Shokryazdan, Parisa The main objective of the present study was to extract and characterize oligosaccharides from palm kernel cake (OligoPKC) to be used as a prebiotic. Up to 16.81% of oligosaccharides were extracted from PKC using neutral detergent solution with two to eight degrees of polymerization. Molecular weights of seven fractions of OligoPKC were estimated using a mass spectrophotometer procedure resembling those of mannobiose, mannotriose, mannotetraose, mannopentaose, and mannohexaose standards, while those of two unknown components resembled those of heptasaccharide and octasaccharide. Enzymatic hydrolysis of OligoPKC using 11 enzymes showed that β-mannosidase and β-mannanase had the highest effects. OligoPKC fractions were potential substrates for growth of four species of Lactobacillus. Supplementation of OligoPKC in the diet of broiler chickens increased the population of beneficial microbes. However, it reduced the populations of pathogenic bacteria in the cecum. Hence, OligoPKC can be considered a potential prebiotic supplement in the feed and food industry. North Carolina State University 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54254/1/Extraction%20and%20characterization%20of%20oligosaccharides%20from%20palm%20kernel%20cake%20as%20prebiotic.pdf Jahromi, Mohammad Faseleh and Liang, Juan Boo and Abdullah, Norhani and Goh, Yong Meng and Ebrahimi, Rohollah and Shokryazdan, Parisa (2016) Extraction and characterization of oligosaccharides from palm kernel cake as prebiotic. BioResources, 11 (1). pp. 674-695. ISSN 1930-2126 http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_1_674_Faseleh_Extraction_Oligosaccharides_Palm_Kernel_Prebiotic Prebiotic; Oligosaccharide; Palm kernel cake
spellingShingle Prebiotic; Oligosaccharide; Palm kernel cake
Jahromi, Mohammad Faseleh
Liang, Juan Boo
Abdullah, Norhani
Goh, Yong Meng
Ebrahimi, Rohollah
Shokryazdan, Parisa
Extraction and characterization of oligosaccharides from palm kernel cake as prebiotic
title Extraction and characterization of oligosaccharides from palm kernel cake as prebiotic
title_full Extraction and characterization of oligosaccharides from palm kernel cake as prebiotic
title_fullStr Extraction and characterization of oligosaccharides from palm kernel cake as prebiotic
title_full_unstemmed Extraction and characterization of oligosaccharides from palm kernel cake as prebiotic
title_short Extraction and characterization of oligosaccharides from palm kernel cake as prebiotic
title_sort extraction and characterization of oligosaccharides from palm kernel cake as prebiotic
topic Prebiotic; Oligosaccharide; Palm kernel cake
url http://psasir.upm.edu.my/id/eprint/54254/
http://psasir.upm.edu.my/id/eprint/54254/
http://psasir.upm.edu.my/id/eprint/54254/1/Extraction%20and%20characterization%20of%20oligosaccharides%20from%20palm%20kernel%20cake%20as%20prebiotic.pdf