Evaluation of commercial soy sauce koji strains of Aspergillus oryzae for γ-aminobutyric acid (GABA) production
In this study, four selected commercial strains of Aspergillus oryzae were collected from soy sauce koji. These A. oryzae strains designated as NSK, NSZ, NSJ and NST shared similar morphological characteristics with the reference strain (A. oryzae FRR 1675) which confirmed them as A. oryzae species....
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| Format: | Article |
| Language: | English |
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Springer-Verlag
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54209/ http://psasir.upm.edu.my/id/eprint/54209/1/Evaluation%20of%20commercial%20soy%20sauce%20koji%20strains%20of%20Aspergillus%20oryzae%20.pdf |
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| author | Ab Kadir, Safuan Wan Mohtar, Wan Abd Al Qadr Imad Mohammad, Rosfarizan Abdul Halim Lim, Sarina Mohammed, Abdulkarim Sabo Saari, Nazamid |
| author_facet | Ab Kadir, Safuan Wan Mohtar, Wan Abd Al Qadr Imad Mohammad, Rosfarizan Abdul Halim Lim, Sarina Mohammed, Abdulkarim Sabo Saari, Nazamid |
| author_sort | Ab Kadir, Safuan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, four selected commercial strains of Aspergillus oryzae were collected from soy sauce koji. These A. oryzae strains designated as NSK, NSZ, NSJ and NST shared similar morphological characteristics with the reference strain (A. oryzae FRR 1675) which confirmed them as A. oryzae species. They were further evaluated for their ability to produce γ-aminobutyric acid (GABA) by cultivating the spore suspension in a broth medium containing 0.4 % (w/v) of glutamic acid as a substrate for GABA production. The results showed that these strains were capable of producing GABA; however, the concentrations differed significantly (P < 0.05) among themselves. Based on the A. oryzae strains, highest GABA concentration was obtained from NSK (194 mg/L) followed by NSZ (63 mg/L), NSJ (51.53 mg/L) and NST (31.66 mg/L). Therefore, A. oryzae NSK was characterized and the sequence was found to be similar to A. oryzae and A. flavus with 99 % similarity. The evolutionary distance (K nuc) between sequences of identical fungal species was calculated and a phylogenetic tree prepared from the K nuc data showed that the isolate belonged to the A. oryzae species. This finding may allow the development of GABA-rich ingredients using A. oryzae NSK as a starter culture for soy sauce production. |
| first_indexed | 2025-11-15T10:38:47Z |
| format | Article |
| id | upm-54209 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:38:47Z |
| publishDate | 2016 |
| publisher | Springer-Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-542092018-03-06T07:59:11Z http://psasir.upm.edu.my/id/eprint/54209/ Evaluation of commercial soy sauce koji strains of Aspergillus oryzae for γ-aminobutyric acid (GABA) production Ab Kadir, Safuan Wan Mohtar, Wan Abd Al Qadr Imad Mohammad, Rosfarizan Abdul Halim Lim, Sarina Mohammed, Abdulkarim Sabo Saari, Nazamid In this study, four selected commercial strains of Aspergillus oryzae were collected from soy sauce koji. These A. oryzae strains designated as NSK, NSZ, NSJ and NST shared similar morphological characteristics with the reference strain (A. oryzae FRR 1675) which confirmed them as A. oryzae species. They were further evaluated for their ability to produce γ-aminobutyric acid (GABA) by cultivating the spore suspension in a broth medium containing 0.4 % (w/v) of glutamic acid as a substrate for GABA production. The results showed that these strains were capable of producing GABA; however, the concentrations differed significantly (P < 0.05) among themselves. Based on the A. oryzae strains, highest GABA concentration was obtained from NSK (194 mg/L) followed by NSZ (63 mg/L), NSJ (51.53 mg/L) and NST (31.66 mg/L). Therefore, A. oryzae NSK was characterized and the sequence was found to be similar to A. oryzae and A. flavus with 99 % similarity. The evolutionary distance (K nuc) between sequences of identical fungal species was calculated and a phylogenetic tree prepared from the K nuc data showed that the isolate belonged to the A. oryzae species. This finding may allow the development of GABA-rich ingredients using A. oryzae NSK as a starter culture for soy sauce production. Springer-Verlag 2016-10 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54209/1/Evaluation%20of%20commercial%20soy%20sauce%20koji%20strains%20of%20Aspergillus%20oryzae%20.pdf Ab Kadir, Safuan and Wan Mohtar, Wan Abd Al Qadr Imad and Mohammad, Rosfarizan and Abdul Halim Lim, Sarina and Mohammed, Abdulkarim Sabo and Saari, Nazamid (2016) Evaluation of commercial soy sauce koji strains of Aspergillus oryzae for γ-aminobutyric acid (GABA) production. Journal of Industrial Microbiology and Biotechnology, 43 (10). pp. 1387-1395. ISSN 1367-5435; ESSN: 1476-5535 https://link.springer.com/article/10.1007%2Fs10295-016-1828-5 Aspergillus oryzae; Glutamic acid; γ-Aminobutyric acid; Fermentation; Soy sauce 10.1007/s10295-016-1828-5 |
| spellingShingle | Aspergillus oryzae; Glutamic acid; γ-Aminobutyric acid; Fermentation; Soy sauce Ab Kadir, Safuan Wan Mohtar, Wan Abd Al Qadr Imad Mohammad, Rosfarizan Abdul Halim Lim, Sarina Mohammed, Abdulkarim Sabo Saari, Nazamid Evaluation of commercial soy sauce koji strains of Aspergillus oryzae for γ-aminobutyric acid (GABA) production |
| title | Evaluation of commercial soy sauce koji strains of Aspergillus oryzae for γ-aminobutyric acid (GABA) production |
| title_full | Evaluation of commercial soy sauce koji strains of Aspergillus oryzae for γ-aminobutyric acid (GABA) production |
| title_fullStr | Evaluation of commercial soy sauce koji strains of Aspergillus oryzae for γ-aminobutyric acid (GABA) production |
| title_full_unstemmed | Evaluation of commercial soy sauce koji strains of Aspergillus oryzae for γ-aminobutyric acid (GABA) production |
| title_short | Evaluation of commercial soy sauce koji strains of Aspergillus oryzae for γ-aminobutyric acid (GABA) production |
| title_sort | evaluation of commercial soy sauce koji strains of aspergillus oryzae for γ-aminobutyric acid (gaba) production |
| topic | Aspergillus oryzae; Glutamic acid; γ-Aminobutyric acid; Fermentation; Soy sauce |
| url | http://psasir.upm.edu.my/id/eprint/54209/ http://psasir.upm.edu.my/id/eprint/54209/ http://psasir.upm.edu.my/id/eprint/54209/ http://psasir.upm.edu.my/id/eprint/54209/1/Evaluation%20of%20commercial%20soy%20sauce%20koji%20strains%20of%20Aspergillus%20oryzae%20.pdf |