Enhancement of lipid production in two marine microalgae under different levels of nitrogen and phosphorus deficiency

Microalgae are important food sources for aquaculture animals. Among the different factors which influence the biochemical composition of microalgae, nitrogen and phosphorus are two of the most important nutrient sources for growth and development. The present study aimed to assess the effects of ni...

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Main Authors: Adenan, Nurul Salma, Md. Yusoff, Fatimah, Medipally, Srikanth Reddy, Mohamed Din, Mohamed Shariff
Format: Article
Language:English
Published: Triveni Enterprises 2016
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/54169/
http://psasir.upm.edu.my/id/eprint/54169/1/Enhancement%20of%20lipid%20production%20in%20two%20marine%20microalgae%20under%20different%20levels%20of%20nitrogen%20and%20phosphorus%20deficiency.pdf
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author Adenan, Nurul Salma
Md. Yusoff, Fatimah
Medipally, Srikanth Reddy
Mohamed Din, Mohamed Shariff
author_facet Adenan, Nurul Salma
Md. Yusoff, Fatimah
Medipally, Srikanth Reddy
Mohamed Din, Mohamed Shariff
author_sort Adenan, Nurul Salma
building UPM Institutional Repository
collection Online Access
description Microalgae are important food sources for aquaculture animals. Among the different factors which influence the biochemical composition of microalgae, nitrogen and phosphorus are two of the most important nutrient sources for growth and development. The present study aimed to assess the effects of nitrogen and phosphorus deficiency on lipid production of Chlorella sp. and Chaetoceros calcitrans. Early stationary phase culture of these species were exposed to different stress levels of nitrogen and phosphorus (25%, 50% and 75% of the full NO3-N and PO4-P concentration in the Conway media), and solvent extraction and gas-liquid chromatography methods were performed for analysis of lipid and fatty acid composition. The results revealed that lipid production in these two species significantly increased (P<0.05) as nitrogen and phosphorus decreased. The fatty acid proportion remained unaffected under nitrogen deficiency, while phosphorus limitation resulted in a decrease of saturated fatty acids and promoted a higher content of omega-3 fatty acids in these species. The protein and carbohydrate levels were also altered under limited nutrients. Therefore, these conditions could be used for enhanced lipid production in microalgae for aquaculture and other industrial applications.
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spelling upm-541692018-03-02T01:46:57Z http://psasir.upm.edu.my/id/eprint/54169/ Enhancement of lipid production in two marine microalgae under different levels of nitrogen and phosphorus deficiency Adenan, Nurul Salma Md. Yusoff, Fatimah Medipally, Srikanth Reddy Mohamed Din, Mohamed Shariff Microalgae are important food sources for aquaculture animals. Among the different factors which influence the biochemical composition of microalgae, nitrogen and phosphorus are two of the most important nutrient sources for growth and development. The present study aimed to assess the effects of nitrogen and phosphorus deficiency on lipid production of Chlorella sp. and Chaetoceros calcitrans. Early stationary phase culture of these species were exposed to different stress levels of nitrogen and phosphorus (25%, 50% and 75% of the full NO3-N and PO4-P concentration in the Conway media), and solvent extraction and gas-liquid chromatography methods were performed for analysis of lipid and fatty acid composition. The results revealed that lipid production in these two species significantly increased (P<0.05) as nitrogen and phosphorus decreased. The fatty acid proportion remained unaffected under nitrogen deficiency, while phosphorus limitation resulted in a decrease of saturated fatty acids and promoted a higher content of omega-3 fatty acids in these species. The protein and carbohydrate levels were also altered under limited nutrients. Therefore, these conditions could be used for enhanced lipid production in microalgae for aquaculture and other industrial applications. Triveni Enterprises 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54169/1/Enhancement%20of%20lipid%20production%20in%20two%20marine%20microalgae%20under%20different%20levels%20of%20nitrogen%20and%20phosphorus%20deficiency.pdf Adenan, Nurul Salma and Md. Yusoff, Fatimah and Medipally, Srikanth Reddy and Mohamed Din, Mohamed Shariff (2016) Enhancement of lipid production in two marine microalgae under different levels of nitrogen and phosphorus deficiency. Journal of Environmental Biology, 37 (spec. 4). pp. 669-676. ISSN 0254-8704; ESSN: 2394-0379 http://www.jeb.co.in/index.php?page=abstract&issue=201607_jul16_spl&number=5 Chaetoceros calcitrans; Chlorella; Lipid; Marine microalgae; Nutrient stress; Omega-3 fatty acids
spellingShingle Chaetoceros calcitrans; Chlorella; Lipid; Marine microalgae; Nutrient stress; Omega-3 fatty acids
Adenan, Nurul Salma
Md. Yusoff, Fatimah
Medipally, Srikanth Reddy
Mohamed Din, Mohamed Shariff
Enhancement of lipid production in two marine microalgae under different levels of nitrogen and phosphorus deficiency
title Enhancement of lipid production in two marine microalgae under different levels of nitrogen and phosphorus deficiency
title_full Enhancement of lipid production in two marine microalgae under different levels of nitrogen and phosphorus deficiency
title_fullStr Enhancement of lipid production in two marine microalgae under different levels of nitrogen and phosphorus deficiency
title_full_unstemmed Enhancement of lipid production in two marine microalgae under different levels of nitrogen and phosphorus deficiency
title_short Enhancement of lipid production in two marine microalgae under different levels of nitrogen and phosphorus deficiency
title_sort enhancement of lipid production in two marine microalgae under different levels of nitrogen and phosphorus deficiency
topic Chaetoceros calcitrans; Chlorella; Lipid; Marine microalgae; Nutrient stress; Omega-3 fatty acids
url http://psasir.upm.edu.my/id/eprint/54169/
http://psasir.upm.edu.my/id/eprint/54169/
http://psasir.upm.edu.my/id/eprint/54169/1/Enhancement%20of%20lipid%20production%20in%20two%20marine%20microalgae%20under%20different%20levels%20of%20nitrogen%20and%20phosphorus%20deficiency.pdf