Enhanced sensing of dengue virus DNA detection using O2 plasma treated-silicon nanowire based electrical biosensor
Dengue Virus (DENV) has become one of the most serious arthropod-borne viral diseases, causing death globally. The existing methods for DENV detection suffer from the late stage treatment due to antibodies-based detection which is feasible only after five days following the onset of the illness. Her...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016
|
| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/54164/ http://psasir.upm.edu.my/id/eprint/54164/1/Enhanced%20sensing%20of%20dengue%20virus%20DNA%20detection%20using%20O2%20plasma%20treated-silicon%20nanowire%20based%20electrical%20biosensor.pdf |
| _version_ | 1848852471105978368 |
|---|---|
| author | Rahman, S. F. A. Yusof, Nor Azah Hashim, Uda Hushiarian, Roy Roozbeh Md Nor, Mohammad Nuzaihan Hamidon, Mohd Nizar Mohd Zawawi, Ruzniza Mohamad Fathil, Mohamad Faris |
| author_facet | Rahman, S. F. A. Yusof, Nor Azah Hashim, Uda Hushiarian, Roy Roozbeh Md Nor, Mohammad Nuzaihan Hamidon, Mohd Nizar Mohd Zawawi, Ruzniza Mohamad Fathil, Mohamad Faris |
| author_sort | Rahman, S. F. A. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Dengue Virus (DENV) has become one of the most serious arthropod-borne viral diseases, causing death globally. The existing methods for DENV detection suffer from the late stage treatment due to antibodies-based detection which is feasible only after five days following the onset of the illness. Here, we demonstrated the highly effective molecular electronic based detection utilizing silicon nanowire (SiNW) integrated with standard complementary metal-oxide-semiconductor (CMOS) process as a sensing device for detecting deoxyribonucleic acid (DNA) related to DENV in an early stage diagnosis. To transform the fabricated devices as a functional sensing element, three-step procedure consist of SiNW surface modification, DNA immobilization and DNA hybridization were employed. The detection principle works by detecting the changes in current of SiNW which bridge the source and drain terminal to sense the immobilization of probe DNA and their hybridization with target DNA. The oxygen (O2) plasma was proposed as an effective strategy for increasing the binding amounts of target DNA by modified the SiNW surface. It was found that the detection limit of the optimized O2 plasma treated-SiNW device could be reduced to 1.985 × 10−14 M with a linear detection range of the sequence-specific DNA from 1.0 × 10−9 M to 1.0 × 10−13 M. In addition, the developed biosensor device was able to discriminate between complementary, single mismatch and non-complementary DNA sequences. This highly sensitive assay was then applied to the detection of reverse transcription-polymerase chain reaction (RT-PCR) product of DENV-DNA, making it as a potential method for disease diagnosis through electrical biosensor. |
| first_indexed | 2025-11-15T10:38:36Z |
| format | Article |
| id | upm-54164 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:38:36Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-541642018-03-01T08:55:00Z http://psasir.upm.edu.my/id/eprint/54164/ Enhanced sensing of dengue virus DNA detection using O2 plasma treated-silicon nanowire based electrical biosensor Rahman, S. F. A. Yusof, Nor Azah Hashim, Uda Hushiarian, Roy Roozbeh Md Nor, Mohammad Nuzaihan Hamidon, Mohd Nizar Mohd Zawawi, Ruzniza Mohamad Fathil, Mohamad Faris Dengue Virus (DENV) has become one of the most serious arthropod-borne viral diseases, causing death globally. The existing methods for DENV detection suffer from the late stage treatment due to antibodies-based detection which is feasible only after five days following the onset of the illness. Here, we demonstrated the highly effective molecular electronic based detection utilizing silicon nanowire (SiNW) integrated with standard complementary metal-oxide-semiconductor (CMOS) process as a sensing device for detecting deoxyribonucleic acid (DNA) related to DENV in an early stage diagnosis. To transform the fabricated devices as a functional sensing element, three-step procedure consist of SiNW surface modification, DNA immobilization and DNA hybridization were employed. The detection principle works by detecting the changes in current of SiNW which bridge the source and drain terminal to sense the immobilization of probe DNA and their hybridization with target DNA. The oxygen (O2) plasma was proposed as an effective strategy for increasing the binding amounts of target DNA by modified the SiNW surface. It was found that the detection limit of the optimized O2 plasma treated-SiNW device could be reduced to 1.985 × 10−14 M with a linear detection range of the sequence-specific DNA from 1.0 × 10−9 M to 1.0 × 10−13 M. In addition, the developed biosensor device was able to discriminate between complementary, single mismatch and non-complementary DNA sequences. This highly sensitive assay was then applied to the detection of reverse transcription-polymerase chain reaction (RT-PCR) product of DENV-DNA, making it as a potential method for disease diagnosis through electrical biosensor. Elsevier 2016-10 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54164/1/Enhanced%20sensing%20of%20dengue%20virus%20DNA%20detection%20using%20O2%20plasma%20treated-silicon%20nanowire%20based%20electrical%20biosensor.pdf Rahman, S. F. A. and Yusof, Nor Azah and Hashim, Uda and Hushiarian, Roy Roozbeh and Md Nor, Mohammad Nuzaihan and Hamidon, Mohd Nizar and Mohd Zawawi, Ruzniza and Mohamad Fathil, Mohamad Faris (2016) Enhanced sensing of dengue virus DNA detection using O2 plasma treated-silicon nanowire based electrical biosensor. Analytica Chimica Acta, 942. pp. 74-85. ISSN 0003-2670; ESSN: 1873-4324 https://www.sciencedirect.com/science/article/pii/S0003267016310753 Silicon nanowire; Dengue diagnosis; DNA hybridization detection; Plasma surface treatment; Electrical detection 10.1016/j.aca.2016.09.009 |
| spellingShingle | Silicon nanowire; Dengue diagnosis; DNA hybridization detection; Plasma surface treatment; Electrical detection Rahman, S. F. A. Yusof, Nor Azah Hashim, Uda Hushiarian, Roy Roozbeh Md Nor, Mohammad Nuzaihan Hamidon, Mohd Nizar Mohd Zawawi, Ruzniza Mohamad Fathil, Mohamad Faris Enhanced sensing of dengue virus DNA detection using O2 plasma treated-silicon nanowire based electrical biosensor |
| title | Enhanced sensing of dengue virus DNA detection using O2 plasma treated-silicon nanowire based electrical biosensor |
| title_full | Enhanced sensing of dengue virus DNA detection using O2 plasma treated-silicon nanowire based electrical biosensor |
| title_fullStr | Enhanced sensing of dengue virus DNA detection using O2 plasma treated-silicon nanowire based electrical biosensor |
| title_full_unstemmed | Enhanced sensing of dengue virus DNA detection using O2 plasma treated-silicon nanowire based electrical biosensor |
| title_short | Enhanced sensing of dengue virus DNA detection using O2 plasma treated-silicon nanowire based electrical biosensor |
| title_sort | enhanced sensing of dengue virus dna detection using o2 plasma treated-silicon nanowire based electrical biosensor |
| topic | Silicon nanowire; Dengue diagnosis; DNA hybridization detection; Plasma surface treatment; Electrical detection |
| url | http://psasir.upm.edu.my/id/eprint/54164/ http://psasir.upm.edu.my/id/eprint/54164/ http://psasir.upm.edu.my/id/eprint/54164/ http://psasir.upm.edu.my/id/eprint/54164/1/Enhanced%20sensing%20of%20dengue%20virus%20DNA%20detection%20using%20O2%20plasma%20treated-silicon%20nanowire%20based%20electrical%20biosensor.pdf |