Enhanced caffeine degradation by immobilised cells of Leifsonis sp. strain SIU
In a previous study, we isolated Leifsonia sp. strain SIU, a new bacterium from agricultured soil. The bacterium was tested for its ability to degrade caffeine. The isolate was encapsulated in gellan gum and its ability to degrade caffeine was compared with the free cells. The optimal caffeine degra...
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| Format: | Article |
| Language: | English |
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Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54158/ http://psasir.upm.edu.my/id/eprint/54158/1/Enhanced%20caffeine%20degradation%20by%20immobilised%20cells%20of%20Leifsonia%20sp.%20strain%20SIU.pdf |
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| author | Ibrahim, Salihu Abd Shukor, Mohd Yunus Syed, Mohd. Arif Wan Johari, Wan Lutfi Shamaan, Nor Aripin Sabullah, Mohd Khalizan Ahmad, Siti Aqlima |
| author_facet | Ibrahim, Salihu Abd Shukor, Mohd Yunus Syed, Mohd. Arif Wan Johari, Wan Lutfi Shamaan, Nor Aripin Sabullah, Mohd Khalizan Ahmad, Siti Aqlima |
| author_sort | Ibrahim, Salihu |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In a previous study, we isolated Leifsonia sp. strain SIU, a new bacterium from agricultured soil. The bacterium was tested for its ability to degrade caffeine. The isolate was encapsulated in gellan gum and its ability to degrade caffeine was compared with the free cells. The optimal caffeine degradation was attained at a gellan gum concentration of 0.75% (w/v), a bead size of 4 mm diameter, and 250 beads per 100 mL of medium. At a caffeine concentration of 0.1 g/L, immobilised cells of the strain SIU degraded caffeine within 9 h, which is faster when compared to the case of free cells, in which it took 12 h to degrade. The immobilised cells degraded caffeine completely within 39 and 78 h at 0.5 and 1.0 g/L, while the free cells took 72 and 148 h at 0.5 and 1.0 g/L, respectively. At higher caffeine concentrations, immobilised cells exhibited a higher caffeine degradation rate. At concentrations of 1.5 and 2.0 g/L, caffeine-degrading activities of both immobilised and free cells were inhibited. The immobilised cells showed no loss in caffeine-degrading activity after being used repeatedly for nine 24-h cycles. The effect of heavy metals on immobilised cells was also tested. This study showed an increase in caffeine degradation efficiency when the cells were encapsulated in gellan gum. |
| first_indexed | 2025-11-15T10:38:34Z |
| format | Article |
| id | upm-54158 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:38:34Z |
| publishDate | 2016 |
| publisher | Applied Microbiology, Molecular and Cellular Biosciences Research Foundation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-541582018-03-01T07:39:39Z http://psasir.upm.edu.my/id/eprint/54158/ Enhanced caffeine degradation by immobilised cells of Leifsonis sp. strain SIU Ibrahim, Salihu Abd Shukor, Mohd Yunus Syed, Mohd. Arif Wan Johari, Wan Lutfi Shamaan, Nor Aripin Sabullah, Mohd Khalizan Ahmad, Siti Aqlima In a previous study, we isolated Leifsonia sp. strain SIU, a new bacterium from agricultured soil. The bacterium was tested for its ability to degrade caffeine. The isolate was encapsulated in gellan gum and its ability to degrade caffeine was compared with the free cells. The optimal caffeine degradation was attained at a gellan gum concentration of 0.75% (w/v), a bead size of 4 mm diameter, and 250 beads per 100 mL of medium. At a caffeine concentration of 0.1 g/L, immobilised cells of the strain SIU degraded caffeine within 9 h, which is faster when compared to the case of free cells, in which it took 12 h to degrade. The immobilised cells degraded caffeine completely within 39 and 78 h at 0.5 and 1.0 g/L, while the free cells took 72 and 148 h at 0.5 and 1.0 g/L, respectively. At higher caffeine concentrations, immobilised cells exhibited a higher caffeine degradation rate. At concentrations of 1.5 and 2.0 g/L, caffeine-degrading activities of both immobilised and free cells were inhibited. The immobilised cells showed no loss in caffeine-degrading activity after being used repeatedly for nine 24-h cycles. The effect of heavy metals on immobilised cells was also tested. This study showed an increase in caffeine degradation efficiency when the cells were encapsulated in gellan gum. Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54158/1/Enhanced%20caffeine%20degradation%20by%20immobilised%20cells%20of%20Leifsonia%20sp.%20strain%20SIU.pdf Ibrahim, Salihu and Abd Shukor, Mohd Yunus and Syed, Mohd. Arif and Wan Johari, Wan Lutfi and Shamaan, Nor Aripin and Sabullah, Mohd Khalizan and Ahmad, Siti Aqlima (2016) Enhanced caffeine degradation by immobilised cells of Leifsonis sp. strain SIU. The Journal of General and Applied Microbiology, 62 (1). pp. 18-24. ISSN 0022-1260; ESSN: 1349-8037 https://www.jstage.jst.go.jp/article/jgam/62/1/62_18/_article Caffeine; Degradation; Gellan gum; Immobilisation; Leifsonia sp. 10.2323/jgam.62.18 |
| spellingShingle | Caffeine; Degradation; Gellan gum; Immobilisation; Leifsonia sp. Ibrahim, Salihu Abd Shukor, Mohd Yunus Syed, Mohd. Arif Wan Johari, Wan Lutfi Shamaan, Nor Aripin Sabullah, Mohd Khalizan Ahmad, Siti Aqlima Enhanced caffeine degradation by immobilised cells of Leifsonis sp. strain SIU |
| title | Enhanced caffeine degradation by immobilised cells of Leifsonis sp. strain SIU |
| title_full | Enhanced caffeine degradation by immobilised cells of Leifsonis sp. strain SIU |
| title_fullStr | Enhanced caffeine degradation by immobilised cells of Leifsonis sp. strain SIU |
| title_full_unstemmed | Enhanced caffeine degradation by immobilised cells of Leifsonis sp. strain SIU |
| title_short | Enhanced caffeine degradation by immobilised cells of Leifsonis sp. strain SIU |
| title_sort | enhanced caffeine degradation by immobilised cells of leifsonis sp. strain siu |
| topic | Caffeine; Degradation; Gellan gum; Immobilisation; Leifsonia sp. |
| url | http://psasir.upm.edu.my/id/eprint/54158/ http://psasir.upm.edu.my/id/eprint/54158/ http://psasir.upm.edu.my/id/eprint/54158/ http://psasir.upm.edu.my/id/eprint/54158/1/Enhanced%20caffeine%20degradation%20by%20immobilised%20cells%20of%20Leifsonia%20sp.%20strain%20SIU.pdf |