Synthesis, characterization and in Vitro evaluation of manganese ferrite (MnFe2O4) nanopeprintss for their biocompatibility with murine breast cancer cells (4T1)

Manganese ferrite (MnFe2O4) magnetic nanoparticles were successfully prepared by a sol-gel self-combustion technique using iron nitrate and manganese nitrate, followed by calcination at 150 °C for 24 h. Calcined sample was systematically characterized by X-ray diffraction (XRD), Fourier transform in...

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Main Authors: Kanagesan, Samikannu, Ab Aziz, Sidek, Hashim, Mansor, Ismail, Ismayadi, Tamilselvan, Subramani, Mohammed Alitheen, Noorjahan Banu, Swamy, Mallappa Kumara, Rao, Bandaru Purna Chandra
Format: Article
Language:English
Published: M D P I AG 2016
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/53974/
http://psasir.upm.edu.my/id/eprint/53974/1/Synthesis%2C%20characterization%20and%20in%20Vitro%20evaluation%20of%20manganese%20ferrite.pdf
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author Kanagesan, Samikannu
Ab Aziz, Sidek
Hashim, Mansor
Ismail, Ismayadi
Tamilselvan, Subramani
Mohammed Alitheen, Noorjahan Banu
Swamy, Mallappa Kumara
Rao, Bandaru Purna Chandra
author_facet Kanagesan, Samikannu
Ab Aziz, Sidek
Hashim, Mansor
Ismail, Ismayadi
Tamilselvan, Subramani
Mohammed Alitheen, Noorjahan Banu
Swamy, Mallappa Kumara
Rao, Bandaru Purna Chandra
author_sort Kanagesan, Samikannu
building UPM Institutional Repository
collection Online Access
description Manganese ferrite (MnFe2O4) magnetic nanoparticles were successfully prepared by a sol-gel self-combustion technique using iron nitrate and manganese nitrate, followed by calcination at 150 °C for 24 h. Calcined sample was systematically characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and vibrational sample magnetometry (VSM) in order to identify the crystalline phase, functional group, morphology, particle size, shape and magnetic behavior. It was observed that the resultant spinal ferrites obtained at low temperature exhibit single phase, nanoparticle size and good magnetic behavior. The study results have revealed the existence of a potent dose dependent cytotoxic effect of MnFe2O4 nanoparticles against 4T1 cell lines at varying concentrations with IC50 values of 210, 198 and 171 μg/mL after 24 h, 48 h and 72 h of incubation, respectively. Cells exposed to higher concentrations of nanoparticles showed a progressive increase of apoptotic and necrotic activity. Below 125 μg/mL concentration the nanoparticles were biocompatible with 4T1 cells.
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spelling upm-539742018-02-22T09:04:38Z http://psasir.upm.edu.my/id/eprint/53974/ Synthesis, characterization and in Vitro evaluation of manganese ferrite (MnFe2O4) nanopeprintss for their biocompatibility with murine breast cancer cells (4T1) Kanagesan, Samikannu Ab Aziz, Sidek Hashim, Mansor Ismail, Ismayadi Tamilselvan, Subramani Mohammed Alitheen, Noorjahan Banu Swamy, Mallappa Kumara Rao, Bandaru Purna Chandra Manganese ferrite (MnFe2O4) magnetic nanoparticles were successfully prepared by a sol-gel self-combustion technique using iron nitrate and manganese nitrate, followed by calcination at 150 °C for 24 h. Calcined sample was systematically characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and vibrational sample magnetometry (VSM) in order to identify the crystalline phase, functional group, morphology, particle size, shape and magnetic behavior. It was observed that the resultant spinal ferrites obtained at low temperature exhibit single phase, nanoparticle size and good magnetic behavior. The study results have revealed the existence of a potent dose dependent cytotoxic effect of MnFe2O4 nanoparticles against 4T1 cell lines at varying concentrations with IC50 values of 210, 198 and 171 μg/mL after 24 h, 48 h and 72 h of incubation, respectively. Cells exposed to higher concentrations of nanoparticles showed a progressive increase of apoptotic and necrotic activity. Below 125 μg/mL concentration the nanoparticles were biocompatible with 4T1 cells. M D P I AG 2016-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/53974/1/Synthesis%2C%20characterization%20and%20in%20Vitro%20evaluation%20of%20manganese%20ferrite.pdf Kanagesan, Samikannu and Ab Aziz, Sidek and Hashim, Mansor and Ismail, Ismayadi and Tamilselvan, Subramani and Mohammed Alitheen, Noorjahan Banu and Swamy, Mallappa Kumara and Rao, Bandaru Purna Chandra (2016) Synthesis, characterization and in Vitro evaluation of manganese ferrite (MnFe2O4) nanopeprintss for their biocompatibility with murine breast cancer cells (4T1). Molecules, 21 (3). pp. 1-9. ISSN 1420-3049 http://www.mdpi.com/1420-3049/21/3 Manganese ferrite; Nanoparticles; Magnetization; Breast cancer; Cytotoxicity 10.3390/molecules21030312
spellingShingle Manganese ferrite; Nanoparticles; Magnetization; Breast cancer; Cytotoxicity
Kanagesan, Samikannu
Ab Aziz, Sidek
Hashim, Mansor
Ismail, Ismayadi
Tamilselvan, Subramani
Mohammed Alitheen, Noorjahan Banu
Swamy, Mallappa Kumara
Rao, Bandaru Purna Chandra
Synthesis, characterization and in Vitro evaluation of manganese ferrite (MnFe2O4) nanopeprintss for their biocompatibility with murine breast cancer cells (4T1)
title Synthesis, characterization and in Vitro evaluation of manganese ferrite (MnFe2O4) nanopeprintss for their biocompatibility with murine breast cancer cells (4T1)
title_full Synthesis, characterization and in Vitro evaluation of manganese ferrite (MnFe2O4) nanopeprintss for their biocompatibility with murine breast cancer cells (4T1)
title_fullStr Synthesis, characterization and in Vitro evaluation of manganese ferrite (MnFe2O4) nanopeprintss for their biocompatibility with murine breast cancer cells (4T1)
title_full_unstemmed Synthesis, characterization and in Vitro evaluation of manganese ferrite (MnFe2O4) nanopeprintss for their biocompatibility with murine breast cancer cells (4T1)
title_short Synthesis, characterization and in Vitro evaluation of manganese ferrite (MnFe2O4) nanopeprintss for their biocompatibility with murine breast cancer cells (4T1)
title_sort synthesis, characterization and in vitro evaluation of manganese ferrite (mnfe2o4) nanopeprintss for their biocompatibility with murine breast cancer cells (4t1)
topic Manganese ferrite; Nanoparticles; Magnetization; Breast cancer; Cytotoxicity
url http://psasir.upm.edu.my/id/eprint/53974/
http://psasir.upm.edu.my/id/eprint/53974/
http://psasir.upm.edu.my/id/eprint/53974/
http://psasir.upm.edu.my/id/eprint/53974/1/Synthesis%2C%20characterization%20and%20in%20Vitro%20evaluation%20of%20manganese%20ferrite.pdf