Synthesis, characterization and DNA binding activity of a potential DNA intercalator
A novel complex, [Ru(dppz)2(p-MOPIP)]2+ (dppz = dipyrido-[3,2-a:20,30-c]phenazine, p-MOPIP = 2-(4-methoxyphenyl) imidazo[4,5-f][1,10]phenanthroline) has been synthesized and characterized by elemental analysis, 1H Nuclear Magnetic Resonance spectroscopy, mass spectrometry, Fourier Transform Infrare...
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| Format: | Article |
| Language: | English |
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Malaysian Analytical Sciences Society
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53973/ http://psasir.upm.edu.my/id/eprint/53973/1/Synthesis%2C%20characterization%20and%20DNA%20binding%20activity%20of%20a%20potential%20DNA%20intercalator.pdf |
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| author | Harun, Siti Norain Razak, Yaakob Ahmad, Haslina |
| author_facet | Harun, Siti Norain Razak, Yaakob Ahmad, Haslina |
| author_sort | Harun, Siti Norain |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A novel complex, [Ru(dppz)2(p-MOPIP)]2+ (dppz = dipyrido-[3,2-a:20,30-c]phenazine, p-MOPIP = 2-(4-methoxyphenyl) imidazo[4,5-f][1,10]phenanthroline) has been synthesized and characterized by elemental analysis, 1H Nuclear Magnetic Resonance spectroscopy, mass spectrometry, Fourier Transform Infrared analysis, Ultra Violet visible and fluorescence spectroscopy. Herein, the complex was designed by adding p-MOPIP as an intercalating ligand and dppz as the ancillary ligand. The DNA binding properties of the complex with Calf Thymus DNA (CT-DNA) were investigated using spectroscopic methods. The UV-visible absorption band observed at 460 nm corresponded to the metal-to-ligand charge transfer (MLCT) while bands at 358 and 281 nm corresponded to intra-ligand (IL) π-π* transitions of the ligand scaffold in p-MOPIP and dppz. The intrinsic binding constant, Kb for this complex was 1.67 x 106 M-1 and this suggested that this complex, [Ru(dppz)2(p-MOPIP)]2+ bound to DNA via the intercalative mode. Interestingly, the interaction of this complex with CT-DNA also had a ‘molecular light switch’ effect. |
| first_indexed | 2025-11-15T10:37:53Z |
| format | Article |
| id | upm-53973 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:37:53Z |
| publishDate | 2016 |
| publisher | Malaysian Analytical Sciences Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-539732018-02-22T08:52:27Z http://psasir.upm.edu.my/id/eprint/53973/ Synthesis, characterization and DNA binding activity of a potential DNA intercalator Harun, Siti Norain Razak, Yaakob Ahmad, Haslina A novel complex, [Ru(dppz)2(p-MOPIP)]2+ (dppz = dipyrido-[3,2-a:20,30-c]phenazine, p-MOPIP = 2-(4-methoxyphenyl) imidazo[4,5-f][1,10]phenanthroline) has been synthesized and characterized by elemental analysis, 1H Nuclear Magnetic Resonance spectroscopy, mass spectrometry, Fourier Transform Infrared analysis, Ultra Violet visible and fluorescence spectroscopy. Herein, the complex was designed by adding p-MOPIP as an intercalating ligand and dppz as the ancillary ligand. The DNA binding properties of the complex with Calf Thymus DNA (CT-DNA) were investigated using spectroscopic methods. The UV-visible absorption band observed at 460 nm corresponded to the metal-to-ligand charge transfer (MLCT) while bands at 358 and 281 nm corresponded to intra-ligand (IL) π-π* transitions of the ligand scaffold in p-MOPIP and dppz. The intrinsic binding constant, Kb for this complex was 1.67 x 106 M-1 and this suggested that this complex, [Ru(dppz)2(p-MOPIP)]2+ bound to DNA via the intercalative mode. Interestingly, the interaction of this complex with CT-DNA also had a ‘molecular light switch’ effect. Malaysian Analytical Sciences Society 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/53973/1/Synthesis%2C%20characterization%20and%20DNA%20binding%20activity%20of%20a%20potential%20DNA%20intercalator.pdf Harun, Siti Norain and Razak, Yaakob and Ahmad, Haslina (2016) Synthesis, characterization and DNA binding activity of a potential DNA intercalator. Malaysian Journal of Analytical Sciences, 20 (4). pp. 760-769. ISSN 1394-2506 http://www.ukm.my/mjas/mjas2018/ Ruthenium; DNA binding; Molecular light switch 10.17576/mjas-2016-2004-08 |
| spellingShingle | Ruthenium; DNA binding; Molecular light switch Harun, Siti Norain Razak, Yaakob Ahmad, Haslina Synthesis, characterization and DNA binding activity of a potential DNA intercalator |
| title | Synthesis, characterization and DNA binding activity of a potential DNA intercalator |
| title_full | Synthesis, characterization and DNA binding activity of a potential DNA intercalator |
| title_fullStr | Synthesis, characterization and DNA binding activity of a potential DNA intercalator |
| title_full_unstemmed | Synthesis, characterization and DNA binding activity of a potential DNA intercalator |
| title_short | Synthesis, characterization and DNA binding activity of a potential DNA intercalator |
| title_sort | synthesis, characterization and dna binding activity of a potential dna intercalator |
| topic | Ruthenium; DNA binding; Molecular light switch |
| url | http://psasir.upm.edu.my/id/eprint/53973/ http://psasir.upm.edu.my/id/eprint/53973/ http://psasir.upm.edu.my/id/eprint/53973/ http://psasir.upm.edu.my/id/eprint/53973/1/Synthesis%2C%20characterization%20and%20DNA%20binding%20activity%20of%20a%20potential%20DNA%20intercalator.pdf |