Spatiotemporal expression and molecular characterization of miR-344b and miR-344c in the developing mouse brain
MicroRNAs (miRNAs) are small noncoding RNA known to regulate brain development. The expression of two novel miRNAs, namely, miR-344b and miR-344c, was characterized during mouse brain developmental stages in this study. In situ hybridization analysis showed that miR-344b and miR-344c were expressed...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Publishing Corporation
2016
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/53863/ http://psasir.upm.edu.my/id/eprint/53863/1/Spatiotemporal%20Expression.pdf |
| _version_ | 1848852395310710784 |
|---|---|
| author | Leong, Jia Wen Abdullah, Syahril Ling, King Hwa Cheah, Pike See |
| author_facet | Leong, Jia Wen Abdullah, Syahril Ling, King Hwa Cheah, Pike See |
| author_sort | Leong, Jia Wen |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | MicroRNAs (miRNAs) are small noncoding RNA known to regulate brain development. The expression of two novel miRNAs, namely, miR-344b and miR-344c, was characterized during mouse brain developmental stages in this study. In situ hybridization analysis showed that miR-344b and miR-344c were expressed in the germinal layer during embryonic brain developmental stages. In contrast, miR-344b was not detectable in the adult brain while miR-344c was expressed exclusively in the adult olfactory bulb and cerebellar granular layer. Stem-loop RT-qPCR analysis of whole brain RNAs showed that expression of the miR-344b and miR-344c was increased as brain developed throughout the embryonic stage and maintained at adulthood. Further investigation showed that these miRNAs were expressed in adult organs, where miR-344b and miR-344c were highly expressed in pancreas and brain, respectively. Bioinformatics analysis suggested miR-344b and miR-344c targeted Olig2 and Otx2 mRNAs, respectively. However, luciferase experiments demonstrated that these miRNAs did not target Olig2 and Otx2 mRNAs. Further investigation on the locality of miR-344b and miR-344c showed that both miRNAs were localized in nuclei of immature neurons. In conclusion, miR-344b and miR-344c were expressed spatiotemporally during mouse brain developmental stages. |
| first_indexed | 2025-11-15T10:37:24Z |
| format | Article |
| id | upm-53863 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:37:24Z |
| publishDate | 2016 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-538632018-02-15T03:59:22Z http://psasir.upm.edu.my/id/eprint/53863/ Spatiotemporal expression and molecular characterization of miR-344b and miR-344c in the developing mouse brain Leong, Jia Wen Abdullah, Syahril Ling, King Hwa Cheah, Pike See MicroRNAs (miRNAs) are small noncoding RNA known to regulate brain development. The expression of two novel miRNAs, namely, miR-344b and miR-344c, was characterized during mouse brain developmental stages in this study. In situ hybridization analysis showed that miR-344b and miR-344c were expressed in the germinal layer during embryonic brain developmental stages. In contrast, miR-344b was not detectable in the adult brain while miR-344c was expressed exclusively in the adult olfactory bulb and cerebellar granular layer. Stem-loop RT-qPCR analysis of whole brain RNAs showed that expression of the miR-344b and miR-344c was increased as brain developed throughout the embryonic stage and maintained at adulthood. Further investigation showed that these miRNAs were expressed in adult organs, where miR-344b and miR-344c were highly expressed in pancreas and brain, respectively. Bioinformatics analysis suggested miR-344b and miR-344c targeted Olig2 and Otx2 mRNAs, respectively. However, luciferase experiments demonstrated that these miRNAs did not target Olig2 and Otx2 mRNAs. Further investigation on the locality of miR-344b and miR-344c showed that both miRNAs were localized in nuclei of immature neurons. In conclusion, miR-344b and miR-344c were expressed spatiotemporally during mouse brain developmental stages. Hindawi Publishing Corporation 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53863/1/Spatiotemporal%20Expression.pdf Leong, Jia Wen and Abdullah, Syahril and Ling, King Hwa and Cheah, Pike See (2016) Spatiotemporal expression and molecular characterization of miR-344b and miR-344c in the developing mouse brain. Neural Plasticity, 2016 (1951250). pp. 1-16. ISSN 2090-5904; ESSN: 1687-5443 https://www.hindawi.com/journals/np/2016/1951250/abs/ 10.1155/2016/1951250 |
| spellingShingle | Leong, Jia Wen Abdullah, Syahril Ling, King Hwa Cheah, Pike See Spatiotemporal expression and molecular characterization of miR-344b and miR-344c in the developing mouse brain |
| title | Spatiotemporal expression and molecular characterization of miR-344b and miR-344c in the developing mouse brain |
| title_full | Spatiotemporal expression and molecular characterization of miR-344b and miR-344c in the developing mouse brain |
| title_fullStr | Spatiotemporal expression and molecular characterization of miR-344b and miR-344c in the developing mouse brain |
| title_full_unstemmed | Spatiotemporal expression and molecular characterization of miR-344b and miR-344c in the developing mouse brain |
| title_short | Spatiotemporal expression and molecular characterization of miR-344b and miR-344c in the developing mouse brain |
| title_sort | spatiotemporal expression and molecular characterization of mir-344b and mir-344c in the developing mouse brain |
| url | http://psasir.upm.edu.my/id/eprint/53863/ http://psasir.upm.edu.my/id/eprint/53863/ http://psasir.upm.edu.my/id/eprint/53863/ http://psasir.upm.edu.my/id/eprint/53863/1/Spatiotemporal%20Expression.pdf |