Sensitive detection of multiple pathogens using a single DNA probe
A simple but promising electrochemical DNA nanosensor was designed, constructed and applied to differentiate a few food-borne pathogens. The DNA probe was initially designed to have a complementary region in Vibrio parahaemolyticus (VP) genome and to make different hybridization patterns with other...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53794/ http://psasir.upm.edu.my/id/eprint/53794/1/Sensitive%20detection%20of%20multiple%20pathogens%20using%20a%20single%20DNA%20probe.pdf |
| _version_ | 1848852377625427968 |
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| author | Nordin, Noordiana Yusof, Nor Azah Abdullah, Jaafar Radu, Son Hushiarian, Roozbeh |
| author_facet | Nordin, Noordiana Yusof, Nor Azah Abdullah, Jaafar Radu, Son Hushiarian, Roozbeh |
| author_sort | Nordin, Noordiana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A simple but promising electrochemical DNA nanosensor was designed, constructed and applied to differentiate a few food-borne pathogens. The DNA probe was initially designed to have a complementary region in Vibrio parahaemolyticus (VP) genome and to make different hybridization patterns with other selected pathogens. The sensor was based on a screen printed carbon electrode (SPCE) modified with polylactide-stabilized gold nanoparticles (PLA-AuNPs) and methylene blue (MB) was employed as the redox indicator binding better to single-stranded DNA. The immobilization and hybridization events were assessed using differential pulse voltammetry (DPV). The fabricated biosensor was able to specifically distinguish complementary, non-complementary and mismatched oligonucleotides. DNA was measured in the range of 2.0×10−9–2.0×10−13 M with a detection limit of 5.3×10−12 M. The relative standard deviation for 6 replications of DPV measurement of 0.2 µM complementary DNA was 4.88%. The fabricated DNA biosensor was considered stable and portable as indicated by a recovery of more than 80% after a storage period of 6 months at 4–45 °C. Cross-reactivity studies against various food-borne pathogens showed a reliably sensitive detection of VP. |
| first_indexed | 2025-11-15T10:37:07Z |
| format | Article |
| id | upm-53794 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:37:07Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-537942018-02-01T10:23:33Z http://psasir.upm.edu.my/id/eprint/53794/ Sensitive detection of multiple pathogens using a single DNA probe Nordin, Noordiana Yusof, Nor Azah Abdullah, Jaafar Radu, Son Hushiarian, Roozbeh A simple but promising electrochemical DNA nanosensor was designed, constructed and applied to differentiate a few food-borne pathogens. The DNA probe was initially designed to have a complementary region in Vibrio parahaemolyticus (VP) genome and to make different hybridization patterns with other selected pathogens. The sensor was based on a screen printed carbon electrode (SPCE) modified with polylactide-stabilized gold nanoparticles (PLA-AuNPs) and methylene blue (MB) was employed as the redox indicator binding better to single-stranded DNA. The immobilization and hybridization events were assessed using differential pulse voltammetry (DPV). The fabricated biosensor was able to specifically distinguish complementary, non-complementary and mismatched oligonucleotides. DNA was measured in the range of 2.0×10−9–2.0×10−13 M with a detection limit of 5.3×10−12 M. The relative standard deviation for 6 replications of DPV measurement of 0.2 µM complementary DNA was 4.88%. The fabricated DNA biosensor was considered stable and portable as indicated by a recovery of more than 80% after a storage period of 6 months at 4–45 °C. Cross-reactivity studies against various food-borne pathogens showed a reliably sensitive detection of VP. Elsevier 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53794/1/Sensitive%20detection%20of%20multiple%20pathogens%20using%20a%20single%20DNA%20probe.pdf Nordin, Noordiana and Yusof, Nor Azah and Abdullah, Jaafar and Radu, Son and Hushiarian, Roozbeh (2016) Sensitive detection of multiple pathogens using a single DNA probe. Biosensors and Bioelectronics, 86 (1). pp. 398-405. ISSN 0956-5663; ESSN: 1873-4235 https://www.sciencedirect.com/science/article/pii/S0956566316306170 10.1016/j.bios.2016.06.077 |
| spellingShingle | Nordin, Noordiana Yusof, Nor Azah Abdullah, Jaafar Radu, Son Hushiarian, Roozbeh Sensitive detection of multiple pathogens using a single DNA probe |
| title | Sensitive detection of multiple pathogens using a single DNA probe |
| title_full | Sensitive detection of multiple pathogens using a single DNA probe |
| title_fullStr | Sensitive detection of multiple pathogens using a single DNA probe |
| title_full_unstemmed | Sensitive detection of multiple pathogens using a single DNA probe |
| title_short | Sensitive detection of multiple pathogens using a single DNA probe |
| title_sort | sensitive detection of multiple pathogens using a single dna probe |
| url | http://psasir.upm.edu.my/id/eprint/53794/ http://psasir.upm.edu.my/id/eprint/53794/ http://psasir.upm.edu.my/id/eprint/53794/ http://psasir.upm.edu.my/id/eprint/53794/1/Sensitive%20detection%20of%20multiple%20pathogens%20using%20a%20single%20DNA%20probe.pdf |