Screening of various parts of phaleria macrocarpa plant for alpha-glucosidase inhibitory activity
Phaleria macrocarpa is an herbal plant used in Malaysia to enhance vitality. The aim of this study was to screen the α-glucosidase inhibitory activity of different parts (fruit flesh, leaves and stem) of P. macrocarpa. Methanol (polar) and n-hexane (nonpolar) extracts, obtained by room temperature s...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53776/ http://psasir.upm.edu.my/id/eprint/53776/1/Screening%20of%20various%20parts%20of%20phaleria%20macrocarpa%20plant%20for%20alpha-glucosidase%20inhibitory%20activity%20.pdf |
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| author | E., Sabina Sarker, Md Zaidul Islam Ghafoor, Kashif Md. Jaffri, Juliana F., Sahena Babiker, Elfadil E. Perumal, Vikneswari Mirhosseini, Hamed Amid, Mehrnoush Khatib, Alfi |
| author_facet | E., Sabina Sarker, Md Zaidul Islam Ghafoor, Kashif Md. Jaffri, Juliana F., Sahena Babiker, Elfadil E. Perumal, Vikneswari Mirhosseini, Hamed Amid, Mehrnoush Khatib, Alfi |
| author_sort | E., Sabina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Phaleria macrocarpa is an herbal plant used in Malaysia to enhance vitality. The aim of this study was to screen the α-glucosidase inhibitory activity of different parts (fruit flesh, leaves and stem) of P. macrocarpa. Methanol (polar) and n-hexane (nonpolar) extracts, obtained by room temperature solvent extraction, were evaluated for in vitro α-glucosidase activity inhibition. The compounds were identified by using gas chromatography-mass spectrometry (GC-MS) according to their similarity index of >70%, which might be responsible for α-glucosidase inhibitory activity. The methanol extract of the fruit flesh had the highest yield (25.6 ± 0.5%), whereas the n-hexane extract of the stem is more effective against α-glucosidase activity (IC50 0.8 ± 0.1 μg/mL). The fruit flesh (IC501.3 ± 0.2 μg/mL) and leaves (IC501.6 ± 0.6 μg/mL) had also well effectively. The identified metabolites are predominantly phenolics, carbohydrates, triterpenes and organic acids, such as D-fructose, squalene, α-linolenic acid and α-D-glucopyranoside. In-depth chemical profiling using GC-MS was performed for the first time for this plant to assess the likely compounds present in the extract that could be associated with anti-hyperglycemic activity. Of the three parts tested, every part indicates the potential α-glucosidase inhibitory activity and hexane extract of stem showed more inhibitory activity among all extracts. Thus, P. macrocarpa can attenuate hyperglycemia by potently inhibiting carbohydrate hydrolyzing enzymes, making it a viable plant as a source of natural compounds for the management of type 2 diabetes mellitus. |
| first_indexed | 2025-11-15T10:37:03Z |
| format | Article |
| id | upm-53776 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:37:03Z |
| publishDate | 2016 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-537762018-01-23T08:08:05Z http://psasir.upm.edu.my/id/eprint/53776/ Screening of various parts of phaleria macrocarpa plant for alpha-glucosidase inhibitory activity E., Sabina Sarker, Md Zaidul Islam Ghafoor, Kashif Md. Jaffri, Juliana F., Sahena Babiker, Elfadil E. Perumal, Vikneswari Mirhosseini, Hamed Amid, Mehrnoush Khatib, Alfi Phaleria macrocarpa is an herbal plant used in Malaysia to enhance vitality. The aim of this study was to screen the α-glucosidase inhibitory activity of different parts (fruit flesh, leaves and stem) of P. macrocarpa. Methanol (polar) and n-hexane (nonpolar) extracts, obtained by room temperature solvent extraction, were evaluated for in vitro α-glucosidase activity inhibition. The compounds were identified by using gas chromatography-mass spectrometry (GC-MS) according to their similarity index of >70%, which might be responsible for α-glucosidase inhibitory activity. The methanol extract of the fruit flesh had the highest yield (25.6 ± 0.5%), whereas the n-hexane extract of the stem is more effective against α-glucosidase activity (IC50 0.8 ± 0.1 μg/mL). The fruit flesh (IC501.3 ± 0.2 μg/mL) and leaves (IC501.6 ± 0.6 μg/mL) had also well effectively. The identified metabolites are predominantly phenolics, carbohydrates, triterpenes and organic acids, such as D-fructose, squalene, α-linolenic acid and α-D-glucopyranoside. In-depth chemical profiling using GC-MS was performed for the first time for this plant to assess the likely compounds present in the extract that could be associated with anti-hyperglycemic activity. Of the three parts tested, every part indicates the potential α-glucosidase inhibitory activity and hexane extract of stem showed more inhibitory activity among all extracts. Thus, P. macrocarpa can attenuate hyperglycemia by potently inhibiting carbohydrate hydrolyzing enzymes, making it a viable plant as a source of natural compounds for the management of type 2 diabetes mellitus. Wiley 2016-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53776/1/Screening%20of%20various%20parts%20of%20phaleria%20macrocarpa%20plant%20for%20alpha-glucosidase%20inhibitory%20activity%20.pdf E., Sabina and Sarker, Md Zaidul Islam and Ghafoor, Kashif and Md. Jaffri, Juliana and F., Sahena and Babiker, Elfadil E. and Perumal, Vikneswari and Mirhosseini, Hamed and Amid, Mehrnoush and Khatib, Alfi (2016) Screening of various parts of phaleria macrocarpa plant for alpha-glucosidase inhibitory activity. Journal of Food Biochemistry, 40 (2). pp. 201-210. ISSN 0145-8884; ESSN: 1745-4514 http://onlinelibrary.wiley.com/doi/10.1111/jfbc.12212/abstract;jsessionid=339F78195FD949555E71D37C749863A3.f01t03 10.1111/jfbc.12212 |
| spellingShingle | E., Sabina Sarker, Md Zaidul Islam Ghafoor, Kashif Md. Jaffri, Juliana F., Sahena Babiker, Elfadil E. Perumal, Vikneswari Mirhosseini, Hamed Amid, Mehrnoush Khatib, Alfi Screening of various parts of phaleria macrocarpa plant for alpha-glucosidase inhibitory activity |
| title | Screening of various parts of phaleria macrocarpa plant for alpha-glucosidase inhibitory activity |
| title_full | Screening of various parts of phaleria macrocarpa plant for alpha-glucosidase inhibitory activity |
| title_fullStr | Screening of various parts of phaleria macrocarpa plant for alpha-glucosidase inhibitory activity |
| title_full_unstemmed | Screening of various parts of phaleria macrocarpa plant for alpha-glucosidase inhibitory activity |
| title_short | Screening of various parts of phaleria macrocarpa plant for alpha-glucosidase inhibitory activity |
| title_sort | screening of various parts of phaleria macrocarpa plant for alpha-glucosidase inhibitory activity |
| url | http://psasir.upm.edu.my/id/eprint/53776/ http://psasir.upm.edu.my/id/eprint/53776/ http://psasir.upm.edu.my/id/eprint/53776/ http://psasir.upm.edu.my/id/eprint/53776/1/Screening%20of%20various%20parts%20of%20phaleria%20macrocarpa%20plant%20for%20alpha-glucosidase%20inhibitory%20activity%20.pdf |