Screening and identification of quorum sensing degraders from live feed Artemia
Quorum sensing (QS) is bacterial cell-to-cell communication with small signal molecules such as acyl-homoserine lactones (AHL) that control a number of phenotypes including the regulation of virulence determinants in pathogenic bacteria. Therefore, quorum sensing degrader has been suggested as one o...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Triveni Enterprises
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53774/ http://psasir.upm.edu.my/id/eprint/53774/1/Screening%20and%20identification%20of%20quorum%20sensing%20degraders%20from%20live%20feed%20Artemia.pdf |
| _version_ | 1848852372662517760 |
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| author | M. N., Noorashikin Li, L. Y. Abd Karim, Murni Marlina Mohd Daud, Hassan Mohd Ikhsan, Natrah Fatin |
| author_facet | M. N., Noorashikin Li, L. Y. Abd Karim, Murni Marlina Mohd Daud, Hassan Mohd Ikhsan, Natrah Fatin |
| author_sort | M. N., Noorashikin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Quorum sensing (QS) is bacterial cell-to-cell communication with small signal molecules such as acyl-homoserine lactones (AHL) that control a number of phenotypes including the regulation of virulence determinants in pathogenic bacteria. Therefore, quorum sensing degrader has been suggested as one of the biocontrol strategies to fight bacterial infections. In the present study, different bacterial QS degrader strains were isolated from Artemia and screened using Chromobacterium violaceum CV026 bioassay. The results showed that six bacterial strains (four Gram-positive and two Gram-negative) isolated from Artemia were able to degrade AHL in two different in vitro assays. All the strains were later identified through 16S rRNA gene sequencing as Rhodococcus opacus, Strepsporangium roseum, Streptomyces alboniger, Enterobacter clocae and Bacillus litoralis. Highest bacterial AHL degrader, Bacillus litoralis BP-ART/6 fully degraded 10 ppm AHL in 9 hrs. The present study showed that bacterial strains isolated from Artemia can act as a QS degrader. |
| first_indexed | 2025-11-15T10:37:02Z |
| format | Article |
| id | upm-53774 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:37:02Z |
| publishDate | 2016 |
| publisher | Triveni Enterprises |
| recordtype | eprints |
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| spelling | upm-537742018-01-23T05:29:34Z http://psasir.upm.edu.my/id/eprint/53774/ Screening and identification of quorum sensing degraders from live feed Artemia M. N., Noorashikin Li, L. Y. Abd Karim, Murni Marlina Mohd Daud, Hassan Mohd Ikhsan, Natrah Fatin Quorum sensing (QS) is bacterial cell-to-cell communication with small signal molecules such as acyl-homoserine lactones (AHL) that control a number of phenotypes including the regulation of virulence determinants in pathogenic bacteria. Therefore, quorum sensing degrader has been suggested as one of the biocontrol strategies to fight bacterial infections. In the present study, different bacterial QS degrader strains were isolated from Artemia and screened using Chromobacterium violaceum CV026 bioassay. The results showed that six bacterial strains (four Gram-positive and two Gram-negative) isolated from Artemia were able to degrade AHL in two different in vitro assays. All the strains were later identified through 16S rRNA gene sequencing as Rhodococcus opacus, Strepsporangium roseum, Streptomyces alboniger, Enterobacter clocae and Bacillus litoralis. Highest bacterial AHL degrader, Bacillus litoralis BP-ART/6 fully degraded 10 ppm AHL in 9 hrs. The present study showed that bacterial strains isolated from Artemia can act as a QS degrader. Triveni Enterprises 2016-07 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53774/1/Screening%20and%20identification%20of%20quorum%20sensing%20degraders%20from%20live%20feed%20Artemia.pdf M. N., Noorashikin and Li, L. Y. and Abd Karim, Murni Marlina and Mohd Daud, Hassan and Mohd Ikhsan, Natrah Fatin (2016) Screening and identification of quorum sensing degraders from live feed Artemia. Journal of Environmental Biology, 37 (spec. 4). pp. 811-816. ISSN 0254-8704; ESSN: 2394-0379 http://www.jeb.co.in/index.php?page=abstract&issue=201607_jul16_spl&number=21 |
| spellingShingle | M. N., Noorashikin Li, L. Y. Abd Karim, Murni Marlina Mohd Daud, Hassan Mohd Ikhsan, Natrah Fatin Screening and identification of quorum sensing degraders from live feed Artemia |
| title | Screening and identification of quorum sensing degraders from live feed Artemia |
| title_full | Screening and identification of quorum sensing degraders from live feed Artemia |
| title_fullStr | Screening and identification of quorum sensing degraders from live feed Artemia |
| title_full_unstemmed | Screening and identification of quorum sensing degraders from live feed Artemia |
| title_short | Screening and identification of quorum sensing degraders from live feed Artemia |
| title_sort | screening and identification of quorum sensing degraders from live feed artemia |
| url | http://psasir.upm.edu.my/id/eprint/53774/ http://psasir.upm.edu.my/id/eprint/53774/ http://psasir.upm.edu.my/id/eprint/53774/1/Screening%20and%20identification%20of%20quorum%20sensing%20degraders%20from%20live%20feed%20Artemia.pdf |