Identification of cellulolytic bacteria isolated from the termite coptotermes curvignathus (Holmgren)
The aim of the present research was to isolate and identify cellulolytic bacteria from the gut of the local termite Coptotermes curvignathus (Holmgren) present in the vicinity of the University of Putra Malaysia. The isolates were cultured in a medium containing carboxymethyl-cellulose and cellobios...
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| Format: | Article |
| Language: | English English |
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2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/5371/ http://psasir.upm.edu.my/id/eprint/5371/1/Identification%20of%20cellulolytic%20bacteria%20isolated%20from%20the%20termite%20coptotermes%20curvignathus.pdf |
| _version_ | 1848840080313024512 |
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| author | Mohammad Ismail, Mohamad Ramin Alimon, Abdul Razak Abdullah, Norhani |
| author_facet | Mohammad Ismail, Mohamad Ramin Alimon, Abdul Razak Abdullah, Norhani |
| author_sort | Mohammad Ismail, Mohamad Ramin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The aim of the present research was to isolate and identify cellulolytic bacteria from the gut of the local termite Coptotermes curvignathus (Holmgren) present in the vicinity of the University of Putra Malaysia. The isolates were cultured in a medium containing carboxymethyl-cellulose and cellobiose. The bacterial species were tentatively identified by using the Biolog reader as well as the Bergey's manual and later confirmed by 16S rRNA sequence homology. The species were all novel strains and identified as Bacillus cereus strain Razmin A, Enterobacter aerogenes strain Razmin B, Enterobacter cloacae strain Razmin C, Chryseobacterium kwangyangense strain Cb and Acinetobacter strain Raminalimon. Biolog reader was not able to identify one of the bacterial species in which it was identified as C. kwangyangense strain Cb by 16S rRNA sequence homology. The GenBank NCBI-EMBL accession numbers for the bacterial strains are EU294508, EU305608, EU305609, EU169201 and EU332791 for B. cereus Razmin A, E. aerogenes strain Razmin B, E. cloacae strain Razmin C, C. kwangyangense strain Cb and Acinetobacter strain Raminalimon, respectively. PRACTICAL APPLICATIONS The experiment describes the isolation and identification of cellulolytic bacteria from termites, using Biolog reader and polymerase chain reaction technique. Termites are common insects in tropical regions and thrive on wood and cellulolytic materials. Hence, the bacteria isolated may be useful in the degradation of cellulosic materials to increase their digestibility and possibly production of metabolites and enzymes. |
| first_indexed | 2025-11-15T07:21:39Z |
| format | Article |
| id | upm-5371 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:21:39Z |
| publishDate | 2009 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-53712015-10-22T07:51:38Z http://psasir.upm.edu.my/id/eprint/5371/ Identification of cellulolytic bacteria isolated from the termite coptotermes curvignathus (Holmgren) Mohammad Ismail, Mohamad Ramin Alimon, Abdul Razak Abdullah, Norhani The aim of the present research was to isolate and identify cellulolytic bacteria from the gut of the local termite Coptotermes curvignathus (Holmgren) present in the vicinity of the University of Putra Malaysia. The isolates were cultured in a medium containing carboxymethyl-cellulose and cellobiose. The bacterial species were tentatively identified by using the Biolog reader as well as the Bergey's manual and later confirmed by 16S rRNA sequence homology. The species were all novel strains and identified as Bacillus cereus strain Razmin A, Enterobacter aerogenes strain Razmin B, Enterobacter cloacae strain Razmin C, Chryseobacterium kwangyangense strain Cb and Acinetobacter strain Raminalimon. Biolog reader was not able to identify one of the bacterial species in which it was identified as C. kwangyangense strain Cb by 16S rRNA sequence homology. The GenBank NCBI-EMBL accession numbers for the bacterial strains are EU294508, EU305608, EU305609, EU169201 and EU332791 for B. cereus Razmin A, E. aerogenes strain Razmin B, E. cloacae strain Razmin C, C. kwangyangense strain Cb and Acinetobacter strain Raminalimon, respectively. PRACTICAL APPLICATIONS The experiment describes the isolation and identification of cellulolytic bacteria from termites, using Biolog reader and polymerase chain reaction technique. Termites are common insects in tropical regions and thrive on wood and cellulolytic materials. Hence, the bacteria isolated may be useful in the degradation of cellulosic materials to increase their digestibility and possibly production of metabolites and enzymes. 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/5371/1/Identification%20of%20cellulolytic%20bacteria%20isolated%20from%20the%20termite%20coptotermes%20curvignathus.pdf Mohammad Ismail, Mohamad Ramin and Alimon, Abdul Razak and Abdullah, Norhani (2009) Identification of cellulolytic bacteria isolated from the termite coptotermes curvignathus (Holmgren). Journal of Rapid Methods and Automation in Microbiology, 17 (1). pp. 103-116. ISSN 1060-3999 http://dx.doi.org/10.1111/j.1745-4581.2009.00160.x 10.1111/j.1745-4581.2009.00160.x English |
| spellingShingle | Mohammad Ismail, Mohamad Ramin Alimon, Abdul Razak Abdullah, Norhani Identification of cellulolytic bacteria isolated from the termite coptotermes curvignathus (Holmgren) |
| title | Identification of cellulolytic bacteria isolated from the termite coptotermes curvignathus (Holmgren) |
| title_full | Identification of cellulolytic bacteria isolated from the termite coptotermes curvignathus (Holmgren) |
| title_fullStr | Identification of cellulolytic bacteria isolated from the termite coptotermes curvignathus (Holmgren) |
| title_full_unstemmed | Identification of cellulolytic bacteria isolated from the termite coptotermes curvignathus (Holmgren) |
| title_short | Identification of cellulolytic bacteria isolated from the termite coptotermes curvignathus (Holmgren) |
| title_sort | identification of cellulolytic bacteria isolated from the termite coptotermes curvignathus (holmgren) |
| url | http://psasir.upm.edu.my/id/eprint/5371/ http://psasir.upm.edu.my/id/eprint/5371/ http://psasir.upm.edu.my/id/eprint/5371/ http://psasir.upm.edu.my/id/eprint/5371/1/Identification%20of%20cellulolytic%20bacteria%20isolated%20from%20the%20termite%20coptotermes%20curvignathus.pdf |