Prophylactic effects of Clausena excavata Burum. f. leaf extract in ethanol-induced gastric ulcers
Clausena excavata is a natural herb with both antioxidant and anti-inflammatory properties. It has been used for decades in folkloric practice for the amelioration of various ailments. In this study, the gastroprotective activity of methanolic extract of C. excavata leaves (MECE) was determined in t...
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| Language: | English |
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Dove Medical Press
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53688/ http://psasir.upm.edu.my/id/eprint/53688/1/Prophylactic%20effects%20of%20Clausena%20excavata%20Burum.%20f.%20leaf%20extract%20in%20ethanol-induced%20gastric%20ulcers.pdf |
| _version_ | 1848852349694509056 |
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| author | Albaayit, Shaymaa Fadhel Abbas Abba, Yusuf Abdullah, Rasedee Abdullah, Noorlidah |
| author_facet | Albaayit, Shaymaa Fadhel Abbas Abba, Yusuf Abdullah, Rasedee Abdullah, Noorlidah |
| author_sort | Albaayit, Shaymaa Fadhel Abbas |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Clausena excavata is a natural herb with both antioxidant and anti-inflammatory properties. It has been used for decades in folkloric practice for the amelioration of various ailments. In this study, the gastroprotective activity of methanolic extract of C. excavata leaves (MECE) was determined in the Sprague Dawley rat ethanol-induced gastric ulcer model. Rats were pretreated with a single dose of vehicle (5% Tween 20), 20 mg/mL omeprazole, 400 and 200 mg/mL of MECE dissolved in 5% Tween 20. Ulcer was induced with 5 mL/kg of ethanol and stomach tissue was obtained after 1 hour. Histological examination was done on hematoxylin and eosin, periodic acid-Schiff, and immunochemically stained gastric mucosal tissues. Prostaglandin E2, superoxide dismutase, catalase, glutathione peroxidase, and lipid peroxidation levels of the gastric tissue homogenates were also determined. Significantly (P<0.05) smaller ulcer areas, less intense edema, and fewer leukocytes’ infiltration were observed in MECE- and omeprazole-treated than in untreated gastric mucosa with ulcer. The gastric pH, mucus production, superoxide dismutase, catalase, and glutathione peroxidase contents increased, while the lipid peroxidation content decreased as a result of MECE treatment. Bcl-2-associated X protein was underexpressed, while heat shock protein 70 and transforming growth factor-beta protein were overexpressed in the ulcerated gastric mucosa tissues treated with omeprazole and MECE. Similarly, there was a reduction in the levels of tumor necrotic factor-alpha and interleukin-6, while the level of interleukin-10 was increased. This study showed that the gastroprotective effect of MECE is achieved through inhibition of gastric juice secretion and ulcer lesion development, stimulation of mucus secretion, elevation of gastric pH, reduction of reactive oxygen species production, inhibition of apoptosis in the gastric mucosa, and modulation of inflammatory cytokines. |
| first_indexed | 2025-11-15T10:36:40Z |
| format | Article |
| id | upm-53688 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:36:40Z |
| publishDate | 2016 |
| publisher | Dove Medical Press |
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| spelling | upm-536882018-01-04T07:53:31Z http://psasir.upm.edu.my/id/eprint/53688/ Prophylactic effects of Clausena excavata Burum. f. leaf extract in ethanol-induced gastric ulcers Albaayit, Shaymaa Fadhel Abbas Abba, Yusuf Abdullah, Rasedee Abdullah, Noorlidah Clausena excavata is a natural herb with both antioxidant and anti-inflammatory properties. It has been used for decades in folkloric practice for the amelioration of various ailments. In this study, the gastroprotective activity of methanolic extract of C. excavata leaves (MECE) was determined in the Sprague Dawley rat ethanol-induced gastric ulcer model. Rats were pretreated with a single dose of vehicle (5% Tween 20), 20 mg/mL omeprazole, 400 and 200 mg/mL of MECE dissolved in 5% Tween 20. Ulcer was induced with 5 mL/kg of ethanol and stomach tissue was obtained after 1 hour. Histological examination was done on hematoxylin and eosin, periodic acid-Schiff, and immunochemically stained gastric mucosal tissues. Prostaglandin E2, superoxide dismutase, catalase, glutathione peroxidase, and lipid peroxidation levels of the gastric tissue homogenates were also determined. Significantly (P<0.05) smaller ulcer areas, less intense edema, and fewer leukocytes’ infiltration were observed in MECE- and omeprazole-treated than in untreated gastric mucosa with ulcer. The gastric pH, mucus production, superoxide dismutase, catalase, and glutathione peroxidase contents increased, while the lipid peroxidation content decreased as a result of MECE treatment. Bcl-2-associated X protein was underexpressed, while heat shock protein 70 and transforming growth factor-beta protein were overexpressed in the ulcerated gastric mucosa tissues treated with omeprazole and MECE. Similarly, there was a reduction in the levels of tumor necrotic factor-alpha and interleukin-6, while the level of interleukin-10 was increased. This study showed that the gastroprotective effect of MECE is achieved through inhibition of gastric juice secretion and ulcer lesion development, stimulation of mucus secretion, elevation of gastric pH, reduction of reactive oxygen species production, inhibition of apoptosis in the gastric mucosa, and modulation of inflammatory cytokines. Dove Medical Press 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53688/1/Prophylactic%20effects%20of%20Clausena%20excavata%20Burum.%20f.%20leaf%20extract%20in%20ethanol-induced%20gastric%20ulcers.pdf Albaayit, Shaymaa Fadhel Abbas and Abba, Yusuf and Abdullah, Rasedee and Abdullah, Noorlidah (2016) Prophylactic effects of Clausena excavata Burum. f. leaf extract in ethanol-induced gastric ulcers. Drug Design Development and Therapy, 2016 (10). pp. 1973-1986. ISSN 1177-8881 https://www.dovepress.com/prophylactic-effects-of-clausena-excavata-burum-f-leaf-extract-in-etha-peer-reviewed-article-DDDT 10.2147/DDDT.S103993 |
| spellingShingle | Albaayit, Shaymaa Fadhel Abbas Abba, Yusuf Abdullah, Rasedee Abdullah, Noorlidah Prophylactic effects of Clausena excavata Burum. f. leaf extract in ethanol-induced gastric ulcers |
| title | Prophylactic effects of Clausena excavata Burum. f. leaf extract in ethanol-induced gastric ulcers |
| title_full | Prophylactic effects of Clausena excavata Burum. f. leaf extract in ethanol-induced gastric ulcers |
| title_fullStr | Prophylactic effects of Clausena excavata Burum. f. leaf extract in ethanol-induced gastric ulcers |
| title_full_unstemmed | Prophylactic effects of Clausena excavata Burum. f. leaf extract in ethanol-induced gastric ulcers |
| title_short | Prophylactic effects of Clausena excavata Burum. f. leaf extract in ethanol-induced gastric ulcers |
| title_sort | prophylactic effects of clausena excavata burum. f. leaf extract in ethanol-induced gastric ulcers |
| url | http://psasir.upm.edu.my/id/eprint/53688/ http://psasir.upm.edu.my/id/eprint/53688/ http://psasir.upm.edu.my/id/eprint/53688/ http://psasir.upm.edu.my/id/eprint/53688/1/Prophylactic%20effects%20of%20Clausena%20excavata%20Burum.%20f.%20leaf%20extract%20in%20ethanol-induced%20gastric%20ulcers.pdf |