Production of y-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system
Aqueous two-phase system (ATPS) extractive bioconversion provides a technique which integrates bioconversion and purification into a single step process. Extractive bioconversion of gamma-cyclodextrin (γ-CD) from soluble starch with cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) enzyme deriv...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53680/ http://psasir.upm.edu.my/id/eprint/53680/1/Production%20of%20%CE%B3-cyclodextrin%20by%20Bacillus%20cereus%20cyclodextrin%20glycosyltransferase%20using%20extractive%20bioconversion%20in%20polymer-salt%20aqueous%20two-phase%20system.pdf |
| _version_ | 1848852348322971648 |
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| author | Lin, Yu Kiat Show, Pau Loke Yap, Yee Jiun Ariff, Arbakariya Mohammad Annuar, Mohammad Suffian Lai, Oi Ming Tang, Teck Kim Juan, Joon Ching Ling, Tau Chuan |
| author_facet | Lin, Yu Kiat Show, Pau Loke Yap, Yee Jiun Ariff, Arbakariya Mohammad Annuar, Mohammad Suffian Lai, Oi Ming Tang, Teck Kim Juan, Joon Ching Ling, Tau Chuan |
| author_sort | Lin, Yu Kiat |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Aqueous two-phase system (ATPS) extractive bioconversion provides a technique which integrates bioconversion and purification into a single step process. Extractive bioconversion of gamma-cyclodextrin (γ-CD) from soluble starch with cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) enzyme derived from Bacillus cereus was evaluated using polyethylene glycol (PEG)/potassium phosphate based on ATPS. The optimum condition was attained in the ATPS constituted of 30.0% (w/w) PEG 3000 g/mol and 7.0% (w/w) potassium phosphate. A γ-CD concentration of 1.60 mg/mL with a 19% concentration ratio was recovered after 1 h bioconversion process. The γ-CD was mainly partitioned to the top phase (YT = 81.88%), with CGTase partitioning in the salt-rich bottom phase (KCGTase = 0.51). Repetitive batch processes of extractive bioconversion were successfully recycled three times, indicating that this is an environmental friendly and a cost saving technique for γ-CD production and purification. |
| first_indexed | 2025-11-15T10:36:39Z |
| format | Article |
| id | upm-53680 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:36:39Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-536802018-01-04T06:04:31Z http://psasir.upm.edu.my/id/eprint/53680/ Production of y-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system Lin, Yu Kiat Show, Pau Loke Yap, Yee Jiun Ariff, Arbakariya Mohammad Annuar, Mohammad Suffian Lai, Oi Ming Tang, Teck Kim Juan, Joon Ching Ling, Tau Chuan Aqueous two-phase system (ATPS) extractive bioconversion provides a technique which integrates bioconversion and purification into a single step process. Extractive bioconversion of gamma-cyclodextrin (γ-CD) from soluble starch with cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) enzyme derived from Bacillus cereus was evaluated using polyethylene glycol (PEG)/potassium phosphate based on ATPS. The optimum condition was attained in the ATPS constituted of 30.0% (w/w) PEG 3000 g/mol and 7.0% (w/w) potassium phosphate. A γ-CD concentration of 1.60 mg/mL with a 19% concentration ratio was recovered after 1 h bioconversion process. The γ-CD was mainly partitioned to the top phase (YT = 81.88%), with CGTase partitioning in the salt-rich bottom phase (KCGTase = 0.51). Repetitive batch processes of extractive bioconversion were successfully recycled three times, indicating that this is an environmental friendly and a cost saving technique for γ-CD production and purification. Elsevier 2016-06 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53680/1/Production%20of%20%CE%B3-cyclodextrin%20by%20Bacillus%20cereus%20cyclodextrin%20glycosyltransferase%20using%20extractive%20bioconversion%20in%20polymer-salt%20aqueous%20two-phase%20system.pdf Lin, Yu Kiat and Show, Pau Loke and Yap, Yee Jiun and Ariff, Arbakariya and Mohammad Annuar, Mohammad Suffian and Lai, Oi Ming and Tang, Teck Kim and Juan, Joon Ching and Ling, Tau Chuan (2016) Production of y-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system. Journal of Bioscience and Bioengineering, 121 (6). pp. 692-696. ISSN 1389-1723; ESSN: 1347-4421 https://www.sciencedirect.com/science/article/pii/S1389172315004016 10.1016/j.jbiosc.2015.11.001 |
| spellingShingle | Lin, Yu Kiat Show, Pau Loke Yap, Yee Jiun Ariff, Arbakariya Mohammad Annuar, Mohammad Suffian Lai, Oi Ming Tang, Teck Kim Juan, Joon Ching Ling, Tau Chuan Production of y-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system |
| title | Production of y-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system |
| title_full | Production of y-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system |
| title_fullStr | Production of y-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system |
| title_full_unstemmed | Production of y-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system |
| title_short | Production of y-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system |
| title_sort | production of y-cyclodextrin by bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system |
| url | http://psasir.upm.edu.my/id/eprint/53680/ http://psasir.upm.edu.my/id/eprint/53680/ http://psasir.upm.edu.my/id/eprint/53680/ http://psasir.upm.edu.my/id/eprint/53680/1/Production%20of%20%CE%B3-cyclodextrin%20by%20Bacillus%20cereus%20cyclodextrin%20glycosyltransferase%20using%20extractive%20bioconversion%20in%20polymer-salt%20aqueous%20two-phase%20system.pdf |