Prediction and in silico identification of novel B-cells and T-cells epitopes in the S1-spike glycoprotein of M41 and CR88 (793/B) infectious bronchitis virus serotypes for applictaion in peptide vacc
Bioinformatic analysis was used to predict antigenic B-cell and T-cell epitopes within the S1 glycoprotein of M41 and CR88 IBV strains. A conserved linear B-cell epitope peptide, , was identified in M41 IBV strains while three such epitopes types namely, , , and , were predicted in CR88 IBV strains....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53633/ http://psasir.upm.edu.my/id/eprint/53633/1/Prediction%20and%20in%20silico.pdf |
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| author | Bande, Faruku Arshad, Siti Suri Bejo, Mohd Hair Kadkhodaei, Saeid Omar, Abdul Rahman |
| author_facet | Bande, Faruku Arshad, Siti Suri Bejo, Mohd Hair Kadkhodaei, Saeid Omar, Abdul Rahman |
| author_sort | Bande, Faruku |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Bioinformatic analysis was used to predict antigenic B-cell and T-cell epitopes within the S1 glycoprotein of M41 and CR88 IBV strains. A conserved linear B-cell epitope peptide, , was identified in M41 IBV strains while three such epitopes types namely, , , and , were predicted in CR88 IBV strains. Analysis of MHCI binding peptides in M41 IBV strains revealed the presence of 15 antigenic peptides out of which 12 were highly conserved in 96–100% of the total M41 strains analysed. Interestingly three of these peptides, GGPITYKVM208, WFNSLSVSI356, and YLADAGLAI472, relatively had high antigenicity index (>1.0). On the other hand, 11 MHCI binding epitope peptides were identified in CR88 IBV strains. Of these, five peptides were found to be highly conserved with a range between 90% and 97%. However, WFNSLSVSL358, SYNISAASV88, and YNISAASVA89 peptides comparably showed high antigenicity scores (>1.0). Combination of antigenic B-cells and T-cells peptides that are conserved across many strains as approach to evoke humoral and CTL immune response will potentially lead to a broad-based vaccine that could reduce the challenges in using live attenuated vaccine technology in the control of IBV infection in poultry. |
| first_indexed | 2025-11-15T10:36:26Z |
| format | Article |
| id | upm-53633 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:36:26Z |
| publishDate | 2016 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-536332017-11-20T03:57:24Z http://psasir.upm.edu.my/id/eprint/53633/ Prediction and in silico identification of novel B-cells and T-cells epitopes in the S1-spike glycoprotein of M41 and CR88 (793/B) infectious bronchitis virus serotypes for applictaion in peptide vacc Bande, Faruku Arshad, Siti Suri Bejo, Mohd Hair Kadkhodaei, Saeid Omar, Abdul Rahman Bioinformatic analysis was used to predict antigenic B-cell and T-cell epitopes within the S1 glycoprotein of M41 and CR88 IBV strains. A conserved linear B-cell epitope peptide, , was identified in M41 IBV strains while three such epitopes types namely, , , and , were predicted in CR88 IBV strains. Analysis of MHCI binding peptides in M41 IBV strains revealed the presence of 15 antigenic peptides out of which 12 were highly conserved in 96–100% of the total M41 strains analysed. Interestingly three of these peptides, GGPITYKVM208, WFNSLSVSI356, and YLADAGLAI472, relatively had high antigenicity index (>1.0). On the other hand, 11 MHCI binding epitope peptides were identified in CR88 IBV strains. Of these, five peptides were found to be highly conserved with a range between 90% and 97%. However, WFNSLSVSL358, SYNISAASV88, and YNISAASVA89 peptides comparably showed high antigenicity scores (>1.0). Combination of antigenic B-cells and T-cells peptides that are conserved across many strains as approach to evoke humoral and CTL immune response will potentially lead to a broad-based vaccine that could reduce the challenges in using live attenuated vaccine technology in the control of IBV infection in poultry. Hindawi Publishing Corporation 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53633/1/Prediction%20and%20in%20silico.pdf Bande, Faruku and Arshad, Siti Suri and Bejo, Mohd Hair and Kadkhodaei, Saeid and Omar, Abdul Rahman (2016) Prediction and in silico identification of novel B-cells and T-cells epitopes in the S1-spike glycoprotein of M41 and CR88 (793/B) infectious bronchitis virus serotypes for applictaion in peptide vacc. Advances in Bioinformatics, 2016. art. no. 5484972. pp. 1-5. ISSN 1687-8027; ESSN: 1687-8035 https://www.hindawi.com/journals/abi/2016/5484972/abs/ 10.1155/2016/5484972 |
| spellingShingle | Bande, Faruku Arshad, Siti Suri Bejo, Mohd Hair Kadkhodaei, Saeid Omar, Abdul Rahman Prediction and in silico identification of novel B-cells and T-cells epitopes in the S1-spike glycoprotein of M41 and CR88 (793/B) infectious bronchitis virus serotypes for applictaion in peptide vacc |
| title | Prediction and in silico identification of novel B-cells and T-cells epitopes in the S1-spike glycoprotein of M41 and CR88 (793/B) infectious bronchitis virus serotypes for applictaion in peptide vacc |
| title_full | Prediction and in silico identification of novel B-cells and T-cells epitopes in the S1-spike glycoprotein of M41 and CR88 (793/B) infectious bronchitis virus serotypes for applictaion in peptide vacc |
| title_fullStr | Prediction and in silico identification of novel B-cells and T-cells epitopes in the S1-spike glycoprotein of M41 and CR88 (793/B) infectious bronchitis virus serotypes for applictaion in peptide vacc |
| title_full_unstemmed | Prediction and in silico identification of novel B-cells and T-cells epitopes in the S1-spike glycoprotein of M41 and CR88 (793/B) infectious bronchitis virus serotypes for applictaion in peptide vacc |
| title_short | Prediction and in silico identification of novel B-cells and T-cells epitopes in the S1-spike glycoprotein of M41 and CR88 (793/B) infectious bronchitis virus serotypes for applictaion in peptide vacc |
| title_sort | prediction and in silico identification of novel b-cells and t-cells epitopes in the s1-spike glycoprotein of m41 and cr88 (793/b) infectious bronchitis virus serotypes for applictaion in peptide vacc |
| url | http://psasir.upm.edu.my/id/eprint/53633/ http://psasir.upm.edu.my/id/eprint/53633/ http://psasir.upm.edu.my/id/eprint/53633/ http://psasir.upm.edu.my/id/eprint/53633/1/Prediction%20and%20in%20silico.pdf |