Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells
Cyphomandra betacea (C. betacea) belongs to the Solanaceae family. This study was aimed to evaluate the anti-proliferative of C.betacea crude extract against selected cancer cell lines (breast and liver cancer) and to identify the polyphenolics profile (phenolic acids and flavonoids) of C. betacea f...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53558/ http://psasir.upm.edu.my/id/eprint/53558/1/Phenolics%20profile%20and%20anti%20proliferative.pdf |
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| author | Abdul Mutalib, Maisarah Ali, Faisal Othman, Fauziah Ramasamy, Rajesh Rahmat, Asmah |
| author_facet | Abdul Mutalib, Maisarah Ali, Faisal Othman, Fauziah Ramasamy, Rajesh Rahmat, Asmah |
| author_sort | Abdul Mutalib, Maisarah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Cyphomandra betacea (C. betacea) belongs to the Solanaceae family. This study was aimed to evaluate the anti-proliferative of C.betacea crude extract against selected cancer cell lines (breast and liver cancer) and to identify the polyphenolics profile (phenolic acids and flavonoids) of C. betacea fruits. Anti-proliferative effect of the extracts was examined using MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide) assay, followed by cell morphology analysis using acridine orange and propidium iodide double staining.The phenolics profile was characterized using high-performance liquid chromatography (HPLC). C. betacea extract showed a high cytotoxic effect against liver and breast cancer cell lines with the IC50 value of 30 and 80 µg/ml, respectively. Phenolics profiling showed that C. betacea extract has a wide variety of polyphenolic compounds which are the responsible substances underlying the cytotoxic activity. The microscopic examination analysed by acridine orange and propidium iodide double staining showed that viable cells of the liver and breast cells were well rounded, large and intact green nuclei compared with the treated cells that characterized by apoptotic features (shrunken nuclei with a less quantity of cytoplasm).The present study demonstrated that the anti-proliferative properties of C. betacea fruits were partially attributed to the rich phenolics content. It supports the hypothesis that C. betacea fruits have potential as an effective agent in cancer therapy. |
| first_indexed | 2025-11-15T10:36:07Z |
| format | Article |
| id | upm-53558 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:36:07Z |
| publishDate | 2016 |
| publisher | SpringerOpen |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-535582017-11-08T02:01:32Z http://psasir.upm.edu.my/id/eprint/53558/ Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells Abdul Mutalib, Maisarah Ali, Faisal Othman, Fauziah Ramasamy, Rajesh Rahmat, Asmah Cyphomandra betacea (C. betacea) belongs to the Solanaceae family. This study was aimed to evaluate the anti-proliferative of C.betacea crude extract against selected cancer cell lines (breast and liver cancer) and to identify the polyphenolics profile (phenolic acids and flavonoids) of C. betacea fruits. Anti-proliferative effect of the extracts was examined using MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide) assay, followed by cell morphology analysis using acridine orange and propidium iodide double staining.The phenolics profile was characterized using high-performance liquid chromatography (HPLC). C. betacea extract showed a high cytotoxic effect against liver and breast cancer cell lines with the IC50 value of 30 and 80 µg/ml, respectively. Phenolics profiling showed that C. betacea extract has a wide variety of polyphenolic compounds which are the responsible substances underlying the cytotoxic activity. The microscopic examination analysed by acridine orange and propidium iodide double staining showed that viable cells of the liver and breast cells were well rounded, large and intact green nuclei compared with the treated cells that characterized by apoptotic features (shrunken nuclei with a less quantity of cytoplasm).The present study demonstrated that the anti-proliferative properties of C. betacea fruits were partially attributed to the rich phenolics content. It supports the hypothesis that C. betacea fruits have potential as an effective agent in cancer therapy. SpringerOpen 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53558/1/Phenolics%20profile%20and%20anti%20proliferative.pdf Abdul Mutalib, Maisarah and Ali, Faisal and Othman, Fauziah and Ramasamy, Rajesh and Rahmat, Asmah (2016) Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells. SpringerPlus, 5 (2105). pp. 1-13. ISSN 2193-1801 https://search.proquest.com/openview/b9e215e8473816c412157166676fe138/1?pq-origsite=gscholar&cbl=2034663 10.1186/s40064‑016‑3777‑x |
| spellingShingle | Abdul Mutalib, Maisarah Ali, Faisal Othman, Fauziah Ramasamy, Rajesh Rahmat, Asmah Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells |
| title | Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells |
| title_full | Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells |
| title_fullStr | Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells |
| title_full_unstemmed | Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells |
| title_short | Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells |
| title_sort | phenolics profile and anti-proliferative activity of cyphomandra betacea fruit in breast and liver cancer cells |
| url | http://psasir.upm.edu.my/id/eprint/53558/ http://psasir.upm.edu.my/id/eprint/53558/ http://psasir.upm.edu.my/id/eprint/53558/ http://psasir.upm.edu.my/id/eprint/53558/1/Phenolics%20profile%20and%20anti%20proliferative.pdf |