Phenolic rich extract from Clinacanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats
Clinacanthus nutans is used as traditional medicine in Asia but there are limited scientific studies to support its use. In this study, the stem and leaf of C. nutans were extracted using solvents of differing polarities, and their antioxidant capacities were determined using multiple antioxidant as...
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| Language: | English |
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Hindawi Publishing Corporation
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53557/ http://psasir.upm.edu.my/id/eprint/53557/1/Phenolic%20rich%20extract.pdf |
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| author | Sarega, Nadarajan Imam, Mustapha Umar Ooi, Der Jiun Chan, Kim Wei Md Esa, Norhaizan Zawawi, Norhasnida Ismail, Maznah |
| author_facet | Sarega, Nadarajan Imam, Mustapha Umar Ooi, Der Jiun Chan, Kim Wei Md Esa, Norhaizan Zawawi, Norhasnida Ismail, Maznah |
| author_sort | Sarega, Nadarajan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Clinacanthus nutans is used as traditional medicine in Asia but there are limited scientific studies to support its use. In this study, the stem and leaf of C. nutans were extracted using solvents of differing polarities, and their antioxidant capacities were determined using multiple antioxidant assays. The water and aqueous methanolic leaf extracts were further fractionated and their antioxidant capacities and phenolic compositions were tested. Furthermore, the efficacies of the water and aqueous methanolic leaf extracts were tested against hyperlipidemia-induced oxidative stress in rats. Serum and hepatic antioxidant and oxidative stress markers were tested after feeding the rats with high fat diet together with the extracts or simvastatin for 7 weeks. The results indicated that both leaf extracts attenuated oxidative stress through increasing serum antioxidant enzymes activity and upregulating the expression of hepatic antioxidant genes. Multiple phenolic compounds were detected in the extracts and fractions of C. nutans, although protocatechuic acid was one of the most abundant and may have contributed significantly towards the bioactivities of the extracts. However, synergistic effects of different phenolics may have contributed to the overall bioactivities. C. nutans can be a good source of functional ingredients for the management of oxidative stress-related diseases. |
| first_indexed | 2025-11-15T10:36:07Z |
| format | Article |
| id | upm-53557 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:36:07Z |
| publishDate | 2016 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-535572017-11-07T10:02:46Z http://psasir.upm.edu.my/id/eprint/53557/ Phenolic rich extract from Clinacanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats Sarega, Nadarajan Imam, Mustapha Umar Ooi, Der Jiun Chan, Kim Wei Md Esa, Norhaizan Zawawi, Norhasnida Ismail, Maznah Clinacanthus nutans is used as traditional medicine in Asia but there are limited scientific studies to support its use. In this study, the stem and leaf of C. nutans were extracted using solvents of differing polarities, and their antioxidant capacities were determined using multiple antioxidant assays. The water and aqueous methanolic leaf extracts were further fractionated and their antioxidant capacities and phenolic compositions were tested. Furthermore, the efficacies of the water and aqueous methanolic leaf extracts were tested against hyperlipidemia-induced oxidative stress in rats. Serum and hepatic antioxidant and oxidative stress markers were tested after feeding the rats with high fat diet together with the extracts or simvastatin for 7 weeks. The results indicated that both leaf extracts attenuated oxidative stress through increasing serum antioxidant enzymes activity and upregulating the expression of hepatic antioxidant genes. Multiple phenolic compounds were detected in the extracts and fractions of C. nutans, although protocatechuic acid was one of the most abundant and may have contributed significantly towards the bioactivities of the extracts. However, synergistic effects of different phenolics may have contributed to the overall bioactivities. C. nutans can be a good source of functional ingredients for the management of oxidative stress-related diseases. Hindawi Publishing Corporation 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53557/1/Phenolic%20rich%20extract.pdf Sarega, Nadarajan and Imam, Mustapha Umar and Ooi, Der Jiun and Chan, Kim Wei and Md Esa, Norhaizan and Zawawi, Norhasnida and Ismail, Maznah (2016) Phenolic rich extract from Clinacanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats. Oxidative Medicine and Cellular Longevity, 2016. art. no. 4137908. pp. 1-16. ISSN 1942-0900; ESSN: 1942-0994 https://www.hindawi.com/journals/omcl/2016/4137908/abs/ 10.1155/2016/4137908 |
| spellingShingle | Sarega, Nadarajan Imam, Mustapha Umar Ooi, Der Jiun Chan, Kim Wei Md Esa, Norhaizan Zawawi, Norhasnida Ismail, Maznah Phenolic rich extract from Clinacanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats |
| title | Phenolic rich extract from Clinacanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats |
| title_full | Phenolic rich extract from Clinacanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats |
| title_fullStr | Phenolic rich extract from Clinacanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats |
| title_full_unstemmed | Phenolic rich extract from Clinacanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats |
| title_short | Phenolic rich extract from Clinacanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats |
| title_sort | phenolic rich extract from clinacanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats |
| url | http://psasir.upm.edu.my/id/eprint/53557/ http://psasir.upm.edu.my/id/eprint/53557/ http://psasir.upm.edu.my/id/eprint/53557/ http://psasir.upm.edu.my/id/eprint/53557/1/Phenolic%20rich%20extract.pdf |