Performance, yield and characteristics of bioflocculants (UPMBF13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentation
This study compares the distinctive performance, yield and characteristics of bioflocculants (UPMBF13) derived from submerged (SmF) and solid-state fermentation (SSF) of Bacillus subtilis UPMB13. The bioflocculants were subjected to morphological characterization, functional groups determination, mo...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Global Network for Environmental Science and Technology
2016
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/53536/ http://psasir.upm.edu.my/id/eprint/53536/1/Performance%2C%20yield%20and%20characteristics%20of%20bioflocculants.pdf |
| _version_ | 1848852309269807104 |
|---|---|
| author | Zulkeflee, Zufarzaana Shamsuddin, Zulkifli Aris, Ahmad Zaharin Yusoff, Mohd. Kamil Komilis, Dimitrios Sánchez, Antoni |
| author_facet | Zulkeflee, Zufarzaana Shamsuddin, Zulkifli Aris, Ahmad Zaharin Yusoff, Mohd. Kamil Komilis, Dimitrios Sánchez, Antoni |
| author_sort | Zulkeflee, Zufarzaana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study compares the distinctive performance, yield and characteristics of bioflocculants (UPMBF13) derived from submerged (SmF) and solid-state fermentation (SSF) of Bacillus subtilis UPMB13. The bioflocculants were subjected to morphological characterization, functional groups determination, molecular weight measurement, protein content quantification and specific compound identification. The SmF and the SSF strategies yielded an average of 2.70 g l-1 and 1.25 g kg-1 of UPMBF13, respectively. The submerged UPMBF13 resulted in a clear suspension with visible flocs formation, while flocs from the SSF treatment were less apparent. Higher total proteinaceous contents of UPMBF13 from the SSF led to the inferiority in flocculating performances. The produced UPMBF13 consisted of hydroxyl, carboxyl, methoxyl and carbonyl functional groups which contributed to their flocculating abilities. The molecular weights of UPMBF13 were around 10-50 kDa, characterizing them into low-molecular weight bioflocculants. The submerged UPMBF13 were more fibrous in nature than the SSF, giving the submerged UPMBF13 the upper hand in flocculation. SmF was the best fermentation method yielding high performing bioflocculants at a faster rate by the utilization of non-elaborative techniques. SSF, on the other hand, was proven feasible but further improvements are needed. |
| first_indexed | 2025-11-15T10:36:02Z |
| format | Article |
| id | upm-53536 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:36:02Z |
| publishDate | 2016 |
| publisher | Global Network for Environmental Science and Technology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-535362017-11-07T02:06:12Z http://psasir.upm.edu.my/id/eprint/53536/ Performance, yield and characteristics of bioflocculants (UPMBF13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentation Zulkeflee, Zufarzaana Shamsuddin, Zulkifli Aris, Ahmad Zaharin Yusoff, Mohd. Kamil Komilis, Dimitrios Sánchez, Antoni This study compares the distinctive performance, yield and characteristics of bioflocculants (UPMBF13) derived from submerged (SmF) and solid-state fermentation (SSF) of Bacillus subtilis UPMB13. The bioflocculants were subjected to morphological characterization, functional groups determination, molecular weight measurement, protein content quantification and specific compound identification. The SmF and the SSF strategies yielded an average of 2.70 g l-1 and 1.25 g kg-1 of UPMBF13, respectively. The submerged UPMBF13 resulted in a clear suspension with visible flocs formation, while flocs from the SSF treatment were less apparent. Higher total proteinaceous contents of UPMBF13 from the SSF led to the inferiority in flocculating performances. The produced UPMBF13 consisted of hydroxyl, carboxyl, methoxyl and carbonyl functional groups which contributed to their flocculating abilities. The molecular weights of UPMBF13 were around 10-50 kDa, characterizing them into low-molecular weight bioflocculants. The submerged UPMBF13 were more fibrous in nature than the SSF, giving the submerged UPMBF13 the upper hand in flocculation. SmF was the best fermentation method yielding high performing bioflocculants at a faster rate by the utilization of non-elaborative techniques. SSF, on the other hand, was proven feasible but further improvements are needed. Global Network for Environmental Science and Technology 2016-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53536/1/Performance%2C%20yield%20and%20characteristics%20of%20bioflocculants.pdf Zulkeflee, Zufarzaana and Shamsuddin, Zulkifli and Aris, Ahmad Zaharin and Yusoff, Mohd. Kamil and Komilis, Dimitrios and Sánchez, Antoni (2016) Performance, yield and characteristics of bioflocculants (UPMBF13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentation. Global NEST Journal, 18 (3). pp. 611-620. ISSN 1790-7632 https://journal.gnest.org/journal-paper/performance-yield-and-characteristics-bioflocculants-upmbf13-produced-bacillus |
| spellingShingle | Zulkeflee, Zufarzaana Shamsuddin, Zulkifli Aris, Ahmad Zaharin Yusoff, Mohd. Kamil Komilis, Dimitrios Sánchez, Antoni Performance, yield and characteristics of bioflocculants (UPMBF13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentation |
| title | Performance, yield and characteristics of bioflocculants (UPMBF13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentation |
| title_full | Performance, yield and characteristics of bioflocculants (UPMBF13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentation |
| title_fullStr | Performance, yield and characteristics of bioflocculants (UPMBF13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentation |
| title_full_unstemmed | Performance, yield and characteristics of bioflocculants (UPMBF13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentation |
| title_short | Performance, yield and characteristics of bioflocculants (UPMBF13) produced by Bacillus subtilis UPMB13 during submerged and solid-state fermentation |
| title_sort | performance, yield and characteristics of bioflocculants (upmbf13) produced by bacillus subtilis upmb13 during submerged and solid-state fermentation |
| url | http://psasir.upm.edu.my/id/eprint/53536/ http://psasir.upm.edu.my/id/eprint/53536/ http://psasir.upm.edu.my/id/eprint/53536/1/Performance%2C%20yield%20and%20characteristics%20of%20bioflocculants.pdf |