Patterned array of poly(ethylene glycol) silane monolayer for label-free detection of dengue
In the present study, the construction of arrays on silicon for naked-eye detection of DNA dengue was demonstrated. The array was created by exposing a polyethylene glycol (PEG) silane monolayer to 254 nm ultraviolet (UV) light through a photomask. Formation of the PEG silane monolayer and photomodi...
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| Format: | Article |
| Language: | English |
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M D P I AG
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53492/ http://psasir.upm.edu.my/id/eprint/53492/1/Patterned%20array.pdf |
| _version_ | 1848852298593206272 |
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| author | Rosly, Nor Zida Alang Ahmad, Shahrul Ainliah Abdullah, Jaafar Yusof, Nor Azah |
| author_facet | Rosly, Nor Zida Alang Ahmad, Shahrul Ainliah Abdullah, Jaafar Yusof, Nor Azah |
| author_sort | Rosly, Nor Zida |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In the present study, the construction of arrays on silicon for naked-eye detection of DNA dengue was demonstrated. The array was created by exposing a polyethylene glycol (PEG) silane monolayer to 254 nm ultraviolet (UV) light through a photomask. Formation of the PEG silane monolayer and photomodifed surface properties was thoroughly characterized by using atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. The results of XPS confirmed that irradiation of ultraviolet (UV) light generates an aldehyde functional group that offers conjugation sites of amino DNA probe for detection of a specific dengue virus target DNA. Employing a gold enhancement process after inducing the electrostatic interaction between positively charged gold nanoparticles and the negatively charged target DNA hybridized to the DNA capture probe allowed to visualize the array with naked eye. The developed arrays demonstrated excellent performance in diagnosis of dengue with a detection limit as low as 10 pM. The selectivity of DNA arrays was also examined using a single base mismatch and noncomplementary target DNA. |
| first_indexed | 2025-11-15T10:35:51Z |
| format | Article |
| id | upm-53492 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:35:51Z |
| publishDate | 2016 |
| publisher | M D P I AG |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-534922017-11-02T07:51:19Z http://psasir.upm.edu.my/id/eprint/53492/ Patterned array of poly(ethylene glycol) silane monolayer for label-free detection of dengue Rosly, Nor Zida Alang Ahmad, Shahrul Ainliah Abdullah, Jaafar Yusof, Nor Azah In the present study, the construction of arrays on silicon for naked-eye detection of DNA dengue was demonstrated. The array was created by exposing a polyethylene glycol (PEG) silane monolayer to 254 nm ultraviolet (UV) light through a photomask. Formation of the PEG silane monolayer and photomodifed surface properties was thoroughly characterized by using atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. The results of XPS confirmed that irradiation of ultraviolet (UV) light generates an aldehyde functional group that offers conjugation sites of amino DNA probe for detection of a specific dengue virus target DNA. Employing a gold enhancement process after inducing the electrostatic interaction between positively charged gold nanoparticles and the negatively charged target DNA hybridized to the DNA capture probe allowed to visualize the array with naked eye. The developed arrays demonstrated excellent performance in diagnosis of dengue with a detection limit as low as 10 pM. The selectivity of DNA arrays was also examined using a single base mismatch and noncomplementary target DNA. M D P I AG 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53492/1/Patterned%20array.pdf Rosly, Nor Zida and Alang Ahmad, Shahrul Ainliah and Abdullah, Jaafar and Yusof, Nor Azah (2016) Patterned array of poly(ethylene glycol) silane monolayer for label-free detection of dengue. Sensors, 16 (9). pp. 1-11. ISSN 1424-8220 http://www.mdpi.com/1424-8220/16/9/1365 10.3390/s16091365 |
| spellingShingle | Rosly, Nor Zida Alang Ahmad, Shahrul Ainliah Abdullah, Jaafar Yusof, Nor Azah Patterned array of poly(ethylene glycol) silane monolayer for label-free detection of dengue |
| title | Patterned array of poly(ethylene glycol) silane monolayer for label-free detection of dengue |
| title_full | Patterned array of poly(ethylene glycol) silane monolayer for label-free detection of dengue |
| title_fullStr | Patterned array of poly(ethylene glycol) silane monolayer for label-free detection of dengue |
| title_full_unstemmed | Patterned array of poly(ethylene glycol) silane monolayer for label-free detection of dengue |
| title_short | Patterned array of poly(ethylene glycol) silane monolayer for label-free detection of dengue |
| title_sort | patterned array of poly(ethylene glycol) silane monolayer for label-free detection of dengue |
| url | http://psasir.upm.edu.my/id/eprint/53492/ http://psasir.upm.edu.my/id/eprint/53492/ http://psasir.upm.edu.my/id/eprint/53492/ http://psasir.upm.edu.my/id/eprint/53492/1/Patterned%20array.pdf |