Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway
CBF/DREB1 is a group of transcription factors that are mainly involved in abiotic stress tolerance in plants. They belong to the AP2/ERF superfamily of plant-specific transcription factors. A gene encoding a new member of this group was isolated from ripening oil palm fruit and designated as EgCBF3....
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| Language: | English |
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Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53418/ http://psasir.upm.edu.my/id/eprint/53418/1/Oil%20palm%20EgCBF3%20conferred%20stress%20tolerance%20in%20transgenic%20tomato%20plants%20through%20modulation%20of%20the%20ethylene%20signaling%20pathway.pdf |
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| author | Ebrahimi, Mortaza Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Namasivayam, Parameswari |
| author_facet | Ebrahimi, Mortaza Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Namasivayam, Parameswari |
| author_sort | Ebrahimi, Mortaza |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | CBF/DREB1 is a group of transcription factors that are mainly involved in abiotic stress tolerance in plants. They belong to the AP2/ERF superfamily of plant-specific transcription factors. A gene encoding a new member of this group was isolated from ripening oil palm fruit and designated as EgCBF3. The oil palm fruit demonstrates the characteristics of a climacteric fruit like tomato, in which ethylene has a major impact on the ripening process. A transgenic approach was used for functional characterization of the EgCBF3, using tomato as the model plant. The effects of ectopic expression of EgCBF3 were analyzed based on expression profiling of the ethylene biosynthesis-related genes, anti-freeze proteins (AFPs), abiotic stress tolerance and plant growth and development. The EgCBF3 tomatoes demonstrated altered phenotypes compared to the wild type tomatoes. Delayed leaf senescence and flowering, increased chlorophyll content and abnormal flowering were the consequences of overexpression of EgCBF3 in the transgenic tomatoes. The EgCBF3 tomatoes demonstrated enhanced abiotic stress tolerance under in vitro conditions. Further, transcript levels of ethylene biosynthesis-related genes, including three SlACSs and two SlACOs, were altered in the transgenic plants’ leaves and roots compared to that in the wild type tomato plant. Among the eight AFPs studied in the wounded leaves of the EgCBF3 tomato plants, transcript levels of SlOSM-L, SlNP24, SlPR5L and SlTSRF1 decreased, while expression of the other four, SlCHI3, SlPR1, SlPR-P2 and SlLAP2, were up-regulated. These findings indicate the possible functions of EgCBF3 in plant growth and development as a regulator of ethylene biosynthesis-related and AFP genes, and as a stimulator of abiotic stress tolerance. |
| first_indexed | 2025-11-15T10:35:32Z |
| format | Article |
| id | upm-53418 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:35:32Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-534182017-10-30T05:07:19Z http://psasir.upm.edu.my/id/eprint/53418/ Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway Ebrahimi, Mortaza Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Namasivayam, Parameswari CBF/DREB1 is a group of transcription factors that are mainly involved in abiotic stress tolerance in plants. They belong to the AP2/ERF superfamily of plant-specific transcription factors. A gene encoding a new member of this group was isolated from ripening oil palm fruit and designated as EgCBF3. The oil palm fruit demonstrates the characteristics of a climacteric fruit like tomato, in which ethylene has a major impact on the ripening process. A transgenic approach was used for functional characterization of the EgCBF3, using tomato as the model plant. The effects of ectopic expression of EgCBF3 were analyzed based on expression profiling of the ethylene biosynthesis-related genes, anti-freeze proteins (AFPs), abiotic stress tolerance and plant growth and development. The EgCBF3 tomatoes demonstrated altered phenotypes compared to the wild type tomatoes. Delayed leaf senescence and flowering, increased chlorophyll content and abnormal flowering were the consequences of overexpression of EgCBF3 in the transgenic tomatoes. The EgCBF3 tomatoes demonstrated enhanced abiotic stress tolerance under in vitro conditions. Further, transcript levels of ethylene biosynthesis-related genes, including three SlACSs and two SlACOs, were altered in the transgenic plants’ leaves and roots compared to that in the wild type tomato plant. Among the eight AFPs studied in the wounded leaves of the EgCBF3 tomato plants, transcript levels of SlOSM-L, SlNP24, SlPR5L and SlTSRF1 decreased, while expression of the other four, SlCHI3, SlPR1, SlPR-P2 and SlLAP2, were up-regulated. These findings indicate the possible functions of EgCBF3 in plant growth and development as a regulator of ethylene biosynthesis-related and AFP genes, and as a stimulator of abiotic stress tolerance. Elsevier 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53418/1/Oil%20palm%20EgCBF3%20conferred%20stress%20tolerance%20in%20transgenic%20tomato%20plants%20through%20modulation%20of%20the%20ethylene%20signaling%20pathway.pdf Ebrahimi, Mortaza and Abdullah, Siti Nor Akmar and Abdul Aziz, Maheran and Namasivayam, Parameswari (2016) Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway. Journal of Plant Physiology, 202. pp. 107-120. ISSN 0176-1617; ESSN: 1618-1328 http://www.sciencedirect.com/science/article/pii/S0176161716301213 10.1016/j.jplph.2016.07.001 |
| spellingShingle | Ebrahimi, Mortaza Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Namasivayam, Parameswari Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway |
| title | Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway |
| title_full | Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway |
| title_fullStr | Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway |
| title_full_unstemmed | Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway |
| title_short | Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway |
| title_sort | oil palm egcbf3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway |
| url | http://psasir.upm.edu.my/id/eprint/53418/ http://psasir.upm.edu.my/id/eprint/53418/ http://psasir.upm.edu.my/id/eprint/53418/ http://psasir.upm.edu.my/id/eprint/53418/1/Oil%20palm%20EgCBF3%20conferred%20stress%20tolerance%20in%20transgenic%20tomato%20plants%20through%20modulation%20of%20the%20ethylene%20signaling%20pathway.pdf |