Newcastle disease virus hemagglutinin neuraminidase as a potential cancer targeting agent
The hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) with its immunotherapeutic activities and sialic acid binding abilities is a promising cancer adjuvant. The HN was surfaced displayed on Lactococcus lactis and its cancer targeting ability was investigated via attachment t...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Ivyspring International Publisher
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53382/ http://psasir.upm.edu.my/id/eprint/53382/1/Newcastle%20disease%20virus%20hemagglutinin%20neuraminidase%20as%20a%20potential%20cancer%20targeting%20agent.pdf |
| _version_ | 1848852268111101952 |
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| author | Baradaran, Ali Yusoff, Khatijah Shafee, Norazizah Abdul Rahim, Raha |
| author_facet | Baradaran, Ali Yusoff, Khatijah Shafee, Norazizah Abdul Rahim, Raha |
| author_sort | Baradaran, Ali |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) with its immunotherapeutic activities and sialic acid binding abilities is a promising cancer adjuvant. The HN was surfaced displayed on Lactococcus lactis and its cancer targeting ability was investigated via attachment to the MDA-MB231 breast cancers. To surface display the HN protein on the bacterial cell wall, HN was fused to N-acetylmuraminidase (AcmA) anchoring motif of L. lactis and expressed in Chinese hamster ovary cells. The expressed recombinant fusion proteins were purified and mixed with a culture of L. lactis and Lactobacillus plantarum. Immunofluorescence assay showed the binding of the recombinant HN-AcmA protein on the surface of the bacterial cells. The bacterial cells carrying the HN-AcmA protein interacted with the MDA-MB231 breast cancer cells. Direct and fluorescent microscopy confirmed that L. lactis and Lb. plantarum surface displaying the recombinant HN were attached to the breast cancer MDA-MB231 cells, providing evidence for the potential ability of HN in targeting to cancer cells. |
| first_indexed | 2025-11-15T10:35:22Z |
| format | Article |
| id | upm-53382 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:35:22Z |
| publishDate | 2016 |
| publisher | Ivyspring International Publisher |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-533822017-10-25T08:13:06Z http://psasir.upm.edu.my/id/eprint/53382/ Newcastle disease virus hemagglutinin neuraminidase as a potential cancer targeting agent Baradaran, Ali Yusoff, Khatijah Shafee, Norazizah Abdul Rahim, Raha The hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) with its immunotherapeutic activities and sialic acid binding abilities is a promising cancer adjuvant. The HN was surfaced displayed on Lactococcus lactis and its cancer targeting ability was investigated via attachment to the MDA-MB231 breast cancers. To surface display the HN protein on the bacterial cell wall, HN was fused to N-acetylmuraminidase (AcmA) anchoring motif of L. lactis and expressed in Chinese hamster ovary cells. The expressed recombinant fusion proteins were purified and mixed with a culture of L. lactis and Lactobacillus plantarum. Immunofluorescence assay showed the binding of the recombinant HN-AcmA protein on the surface of the bacterial cells. The bacterial cells carrying the HN-AcmA protein interacted with the MDA-MB231 breast cancer cells. Direct and fluorescent microscopy confirmed that L. lactis and Lb. plantarum surface displaying the recombinant HN were attached to the breast cancer MDA-MB231 cells, providing evidence for the potential ability of HN in targeting to cancer cells. Ivyspring International Publisher 2016-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53382/1/Newcastle%20disease%20virus%20hemagglutinin%20neuraminidase%20as%20a%20potential%20cancer%20targeting%20agent.pdf Baradaran, Ali and Yusoff, Khatijah and Shafee, Norazizah and Abdul Rahim, Raha (2016) Newcastle disease virus hemagglutinin neuraminidase as a potential cancer targeting agent. Journal of Cancer, 7 (4). pp. 462-466. ISSN 1837-9664 http://www.jcancer.org/v07p0462 10.7150/jca.13566 |
| spellingShingle | Baradaran, Ali Yusoff, Khatijah Shafee, Norazizah Abdul Rahim, Raha Newcastle disease virus hemagglutinin neuraminidase as a potential cancer targeting agent |
| title | Newcastle disease virus hemagglutinin neuraminidase as a potential cancer targeting agent |
| title_full | Newcastle disease virus hemagglutinin neuraminidase as a potential cancer targeting agent |
| title_fullStr | Newcastle disease virus hemagglutinin neuraminidase as a potential cancer targeting agent |
| title_full_unstemmed | Newcastle disease virus hemagglutinin neuraminidase as a potential cancer targeting agent |
| title_short | Newcastle disease virus hemagglutinin neuraminidase as a potential cancer targeting agent |
| title_sort | newcastle disease virus hemagglutinin neuraminidase as a potential cancer targeting agent |
| url | http://psasir.upm.edu.my/id/eprint/53382/ http://psasir.upm.edu.my/id/eprint/53382/ http://psasir.upm.edu.my/id/eprint/53382/ http://psasir.upm.edu.my/id/eprint/53382/1/Newcastle%20disease%20virus%20hemagglutinin%20neuraminidase%20as%20a%20potential%20cancer%20targeting%20agent.pdf |