Molecular characterization of Vibrio harveyi virulence associated serine protease and outer membrane protein genes for vaccine development
Vibrio harveyi is the most pathogenic species associated with the infection in a wide range of marine species in aquaculture industries. A few virulence-associated genes have been discovered in V. harveyi. This study reports the cloning, sequence analysis and phylogenetic study of serine protease...
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International Network for Natural Sciences
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53321/ http://psasir.upm.edu.my/id/eprint/53321/1/Molecular%20characterization%20of%20Vibrio%20harveyi%20virulence%20associated%20serine%20protease%20and%20outer%20membrane%20protein%20genes%20for%20vaccine%20development.pdf |
| _version_ | 1848852252032237568 |
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| author | Mohd Aris, Aslizah Md Yasin, Ina Salwany Saad, Mohd Zamri Mohd Daud, Hassan Mohamed Alipiah, Norfarrah |
| author_facet | Mohd Aris, Aslizah Md Yasin, Ina Salwany Saad, Mohd Zamri Mohd Daud, Hassan Mohamed Alipiah, Norfarrah |
| author_sort | Mohd Aris, Aslizah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Vibrio harveyi is the most pathogenic species associated with the infection in a wide range of marine species in aquaculture industries. A few virulence-associated genes have been discovered in V. harveyi. This study reports the cloning, sequence analysis and phylogenetic study of serine protease (VHS) and outer membrane protein (OMP) from a pathogenic V. harveyi, which isolated from a local outbreak of diseased tiger grouper. Molecular identification revealed that VHS and OMP consist of 1368 and 816 base pairs and encoded for mature peptides of 429 and 251 amino acids, respectively. The amino acids sequence identities of VHS was 100% similarity with protease of V. harveyi and OMP was 99% of membrane protein of V. harveyi, as compared to published sequence. Phylogenetic analysis and conserved domain search proposed that VHS is a serine endoprotease DegQ and OMP is an OmpK type. Signal peptide, transmembrane beta-barrel and subcellular localization have supported the findings whereby demonstrated VHS belonged to periplasmic serine protease DegQ, composed one beta-barrel and two alpha-barrels region. OMP displayed six beta-barrels and twelve alpha-barrels suggesting it is belonged to outer membrane integral membrane protein, specifically act as a porin type outer membrane protein. Prediction of antigenic sites revealed that VHS composed 62 sites and OMP have 36 antigenic sites, assuming that they can provoke immune response of the infected hosts. In conclusion, it is strongly suggests that both genes can be potentially used for developing an effective live-attenuated vaccine candidate against vibriosis and further be applied in aquaculture industries.
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| first_indexed | 2025-11-15T10:35:07Z |
| format | Article |
| id | upm-53321 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:35:07Z |
| publishDate | 2016 |
| publisher | International Network for Natural Sciences |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-533212017-11-10T04:53:38Z http://psasir.upm.edu.my/id/eprint/53321/ Molecular characterization of Vibrio harveyi virulence associated serine protease and outer membrane protein genes for vaccine development Mohd Aris, Aslizah Md Yasin, Ina Salwany Saad, Mohd Zamri Mohd Daud, Hassan Mohamed Alipiah, Norfarrah Vibrio harveyi is the most pathogenic species associated with the infection in a wide range of marine species in aquaculture industries. A few virulence-associated genes have been discovered in V. harveyi. This study reports the cloning, sequence analysis and phylogenetic study of serine protease (VHS) and outer membrane protein (OMP) from a pathogenic V. harveyi, which isolated from a local outbreak of diseased tiger grouper. Molecular identification revealed that VHS and OMP consist of 1368 and 816 base pairs and encoded for mature peptides of 429 and 251 amino acids, respectively. The amino acids sequence identities of VHS was 100% similarity with protease of V. harveyi and OMP was 99% of membrane protein of V. harveyi, as compared to published sequence. Phylogenetic analysis and conserved domain search proposed that VHS is a serine endoprotease DegQ and OMP is an OmpK type. Signal peptide, transmembrane beta-barrel and subcellular localization have supported the findings whereby demonstrated VHS belonged to periplasmic serine protease DegQ, composed one beta-barrel and two alpha-barrels region. OMP displayed six beta-barrels and twelve alpha-barrels suggesting it is belonged to outer membrane integral membrane protein, specifically act as a porin type outer membrane protein. Prediction of antigenic sites revealed that VHS composed 62 sites and OMP have 36 antigenic sites, assuming that they can provoke immune response of the infected hosts. In conclusion, it is strongly suggests that both genes can be potentially used for developing an effective live-attenuated vaccine candidate against vibriosis and further be applied in aquaculture industries. International Network for Natural Sciences 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53321/1/Molecular%20characterization%20of%20Vibrio%20harveyi%20virulence%20associated%20serine%20protease%20and%20outer%20membrane%20protein%20genes%20for%20vaccine%20development.pdf Mohd Aris, Aslizah and Md Yasin, Ina Salwany and Saad, Mohd Zamri and Mohd Daud, Hassan and Mohamed Alipiah, Norfarrah (2016) Molecular characterization of Vibrio harveyi virulence associated serine protease and outer membrane protein genes for vaccine development. International Journal of Biosciences, 8 (3). pp. 10-28. ISSN 2220-6655; ESSN: 2222-5234 http://www.innspub.net/ijb/molecular-characterization-of-vibrio-harveyi-virulence-associated-serine-protease-and-outer-membrane-protein-genes-for-vaccine-development/ 10.12692/ijb/8.3.10-28 |
| spellingShingle | Mohd Aris, Aslizah Md Yasin, Ina Salwany Saad, Mohd Zamri Mohd Daud, Hassan Mohamed Alipiah, Norfarrah Molecular characterization of Vibrio harveyi virulence associated serine protease and outer membrane protein genes for vaccine development |
| title | Molecular characterization of Vibrio harveyi virulence associated serine protease and outer membrane protein genes for vaccine development |
| title_full | Molecular characterization of Vibrio harveyi virulence associated serine protease and outer membrane protein genes for vaccine development |
| title_fullStr | Molecular characterization of Vibrio harveyi virulence associated serine protease and outer membrane protein genes for vaccine development |
| title_full_unstemmed | Molecular characterization of Vibrio harveyi virulence associated serine protease and outer membrane protein genes for vaccine development |
| title_short | Molecular characterization of Vibrio harveyi virulence associated serine protease and outer membrane protein genes for vaccine development |
| title_sort | molecular characterization of vibrio harveyi virulence associated serine protease and outer membrane protein genes for vaccine development |
| url | http://psasir.upm.edu.my/id/eprint/53321/ http://psasir.upm.edu.my/id/eprint/53321/ http://psasir.upm.edu.my/id/eprint/53321/ http://psasir.upm.edu.my/id/eprint/53321/1/Molecular%20characterization%20of%20Vibrio%20harveyi%20virulence%20associated%20serine%20protease%20and%20outer%20membrane%20protein%20genes%20for%20vaccine%20development.pdf |