Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia
The Aeromonas spp., which are found mostly in freshwater fishes are known to cause uncontrolled disease outbreak in aquaculture system. In the present study, twenty-two of Aeromonas spp. strains isolated from diseased freshwater fishes were identified using the 16S ribosomal RNA (rRNA) and internal...
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| Format: | Article |
| Language: | English |
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Excellent Publishers
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53319/ http://psasir.upm.edu.my/id/eprint/53319/1/Molecular%20characterization%20of%2016S%20rRNA%20and%20internal%20transcribed%20spacer%20%28ITS%29%20regions%20of%20Aeromonas%20spp.%20isolated%20from%20cultured%20freshwater%20fishes%20in%20Malaysia.pdf |
| _version_ | 1848852251752267776 |
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| author | K.P., Diyana Nadhirah Md Yasin, Ina Salwany |
| author_facet | K.P., Diyana Nadhirah Md Yasin, Ina Salwany |
| author_sort | K.P., Diyana Nadhirah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The Aeromonas spp., which are found mostly in freshwater fishes are known to cause uncontrolled disease outbreak in aquaculture system. In the present study, twenty-two of Aeromonas spp. strains isolated from diseased freshwater fishes were identified using the 16S ribosomal RNA (rRNA) and internal transcribed spacer (ITS) regions gene amplification where they produced a 1500 bp and 1000-1200 bp amplicon each. Phylogenetic analysis with BLASTn and 16S rRNA neighbor-joining bootstrapping methods revealed that all strains were identified up to genus level with 15 strains were classified as Aeromonas hydrophila (A. hydrophila) and the rest were Aeromonas veronii (A. veronii). Further ITS gene analysis disclosed strains identification up to species level; A. hydrophila (14 strains) and A. veronii (8 strains). These rapid DNA-based detection methods are simple, easy to perform and faster in identifying Aeromonas spp., thus are absolutely efficient for regular monitoring of Aeromonas spp. in a potential outbreak situation. |
| first_indexed | 2025-11-15T10:35:07Z |
| format | Article |
| id | upm-53319 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:35:07Z |
| publishDate | 2016 |
| publisher | Excellent Publishers |
| recordtype | eprints |
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| spelling | upm-533192017-11-10T04:47:44Z http://psasir.upm.edu.my/id/eprint/53319/ Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia K.P., Diyana Nadhirah Md Yasin, Ina Salwany The Aeromonas spp., which are found mostly in freshwater fishes are known to cause uncontrolled disease outbreak in aquaculture system. In the present study, twenty-two of Aeromonas spp. strains isolated from diseased freshwater fishes were identified using the 16S ribosomal RNA (rRNA) and internal transcribed spacer (ITS) regions gene amplification where they produced a 1500 bp and 1000-1200 bp amplicon each. Phylogenetic analysis with BLASTn and 16S rRNA neighbor-joining bootstrapping methods revealed that all strains were identified up to genus level with 15 strains were classified as Aeromonas hydrophila (A. hydrophila) and the rest were Aeromonas veronii (A. veronii). Further ITS gene analysis disclosed strains identification up to species level; A. hydrophila (14 strains) and A. veronii (8 strains). These rapid DNA-based detection methods are simple, easy to perform and faster in identifying Aeromonas spp., thus are absolutely efficient for regular monitoring of Aeromonas spp. in a potential outbreak situation. Excellent Publishers 2016-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53319/1/Molecular%20characterization%20of%2016S%20rRNA%20and%20internal%20transcribed%20spacer%20%28ITS%29%20regions%20of%20Aeromonas%20spp.%20isolated%20from%20cultured%20freshwater%20fishes%20in%20Malaysia.pdf K.P., Diyana Nadhirah and Md Yasin, Ina Salwany (2016) Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia. International Journal of Current Microbiology and Applied Sciences, 5 (9). pp. 431-440. ISSN 2319-7692; ESSN: 2319-7706 https://www.ijcmas.com/abstractview.php?ID=887&vol=5-9-2016&SNo=46 10.20546/ijcmas.2016.509.046 |
| spellingShingle | K.P., Diyana Nadhirah Md Yasin, Ina Salwany Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia |
| title | Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia |
| title_full | Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia |
| title_fullStr | Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia |
| title_full_unstemmed | Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia |
| title_short | Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia |
| title_sort | molecular characterization of 16s rrna and internal transcribed spacer (its) regions of aeromonas spp. isolated from cultured freshwater fishes in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/53319/ http://psasir.upm.edu.my/id/eprint/53319/ http://psasir.upm.edu.my/id/eprint/53319/ http://psasir.upm.edu.my/id/eprint/53319/1/Molecular%20characterization%20of%2016S%20rRNA%20and%20internal%20transcribed%20spacer%20%28ITS%29%20regions%20of%20Aeromonas%20spp.%20isolated%20from%20cultured%20freshwater%20fishes%20in%20Malaysia.pdf |