Modelling the effect of picloram on the growth kinetics of cell suspension cultures of Ficus deltoidea L.

Ficus deltoidea (or commonly known as mistletoe fig) in various parts of the world mainly serves as an ornamental shrub or houseplant and found native mainly in Asia tropical region, for example, Malaysia, Indonesia, Philippines and Thailand. Studies on the effect of plant growth regulators on cells...

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Main Authors: Pick, Anna Kiong Ling, Halmi, Mohd Izuan Effendi, Hussein, Sobri, Ong, S.L.
Format: Article
Language:English
Published: Hibiscus Publisher 2016
Online Access:http://psasir.upm.edu.my/id/eprint/53310/
http://psasir.upm.edu.my/id/eprint/53310/1/Modelling%20the%20effect%20of%20picloram%20on%20the%20growth%20kinetics%20of%20cell%20suspension%20cultures%20of%20Ficus%20deltoidea%20L..pdf
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author Pick, Anna Kiong Ling
Halmi, Mohd Izuan Effendi
Hussein, Sobri
Ong, S.L.
author_facet Pick, Anna Kiong Ling
Halmi, Mohd Izuan Effendi
Hussein, Sobri
Ong, S.L.
author_sort Pick, Anna Kiong Ling
building UPM Institutional Repository
collection Online Access
description Ficus deltoidea (or commonly known as mistletoe fig) in various parts of the world mainly serves as an ornamental shrub or houseplant and found native mainly in Asia tropical region, for example, Malaysia, Indonesia, Philippines and Thailand. Studies on the effect of plant growth regulators on cells production from this plant is important as optimization of cells production can lead to efficient production of secondary products characterization and production. An important aspect of the sigmoidal cells growth curve is the growth parameters. In this work, we model the effect of picloram (4-amino-3,5,6-trichloropicolinic acid) on the growth kinetics of the cell suspension cultures of Ficus deltoidea according to the modified Gompertz model. The adjusted coefficient of determination showed good agreement between experimental and predicted data with values ranging from 0.97-0.99. Parameters obtained from the fitting exercise were maximum cells growth rate (µm), lag time (λ) and maximal cells production (Ymax). The results showed that picloram at concentrations of 3 mg/L and above were optimal for giving the highest cells growth rate measured as PCV (packed cell volume). The parameter growth rate obtained from the modelling exercise will be helpful for additional secondary modelling implicating the consequence of media conditions as well as other factors on the effect of picloram on the growth rate of cell suspension from this plant.
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spelling upm-533102022-03-18T05:42:02Z http://psasir.upm.edu.my/id/eprint/53310/ Modelling the effect of picloram on the growth kinetics of cell suspension cultures of Ficus deltoidea L. Pick, Anna Kiong Ling Halmi, Mohd Izuan Effendi Hussein, Sobri Ong, S.L. Ficus deltoidea (or commonly known as mistletoe fig) in various parts of the world mainly serves as an ornamental shrub or houseplant and found native mainly in Asia tropical region, for example, Malaysia, Indonesia, Philippines and Thailand. Studies on the effect of plant growth regulators on cells production from this plant is important as optimization of cells production can lead to efficient production of secondary products characterization and production. An important aspect of the sigmoidal cells growth curve is the growth parameters. In this work, we model the effect of picloram (4-amino-3,5,6-trichloropicolinic acid) on the growth kinetics of the cell suspension cultures of Ficus deltoidea according to the modified Gompertz model. The adjusted coefficient of determination showed good agreement between experimental and predicted data with values ranging from 0.97-0.99. Parameters obtained from the fitting exercise were maximum cells growth rate (µm), lag time (λ) and maximal cells production (Ymax). The results showed that picloram at concentrations of 3 mg/L and above were optimal for giving the highest cells growth rate measured as PCV (packed cell volume). The parameter growth rate obtained from the modelling exercise will be helpful for additional secondary modelling implicating the consequence of media conditions as well as other factors on the effect of picloram on the growth rate of cell suspension from this plant. Hibiscus Publisher 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/53310/1/Modelling%20the%20effect%20of%20picloram%20on%20the%20growth%20kinetics%20of%20cell%20suspension%20cultures%20of%20Ficus%20deltoidea%20L..pdf Pick, Anna Kiong Ling and Halmi, Mohd Izuan Effendi and Hussein, Sobri and Ong, S.L. (2016) Modelling the effect of picloram on the growth kinetics of cell suspension cultures of Ficus deltoidea L. Journal of Biochemistry, Microbiology and Biotechnology, 4 (1). pp. 24-28. ISSN 2289-5779 https://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/285 10.54987/jobimb.v4i1.285
spellingShingle Pick, Anna Kiong Ling
Halmi, Mohd Izuan Effendi
Hussein, Sobri
Ong, S.L.
Modelling the effect of picloram on the growth kinetics of cell suspension cultures of Ficus deltoidea L.
title Modelling the effect of picloram on the growth kinetics of cell suspension cultures of Ficus deltoidea L.
title_full Modelling the effect of picloram on the growth kinetics of cell suspension cultures of Ficus deltoidea L.
title_fullStr Modelling the effect of picloram on the growth kinetics of cell suspension cultures of Ficus deltoidea L.
title_full_unstemmed Modelling the effect of picloram on the growth kinetics of cell suspension cultures of Ficus deltoidea L.
title_short Modelling the effect of picloram on the growth kinetics of cell suspension cultures of Ficus deltoidea L.
title_sort modelling the effect of picloram on the growth kinetics of cell suspension cultures of ficus deltoidea l.
url http://psasir.upm.edu.my/id/eprint/53310/
http://psasir.upm.edu.my/id/eprint/53310/
http://psasir.upm.edu.my/id/eprint/53310/
http://psasir.upm.edu.my/id/eprint/53310/1/Modelling%20the%20effect%20of%20picloram%20on%20the%20growth%20kinetics%20of%20cell%20suspension%20cultures%20of%20Ficus%20deltoidea%20L..pdf