Minimal inhibitory concentration of common selectable agents on recalcitrant Malaysian rice cultivar
Determining the suitable and optimized Minimal Inhibitory Concentration (MIC) of the selectable agents is critical to successfully recover stably transformed plants. In this study, the MIC of five different antibiotics and one herbicide was determined for their inhibitory effect on the growth of a r...
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| Format: | Article |
| Language: | English |
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Science Alert
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53288/ http://psasir.upm.edu.my/id/eprint/53288/1/Minimal%20inhibitory%20concentration%20of%20common%20selectable%20agents%20on%20recalcitrant%20Malaysian%20rice%20cultivar.pdf |
| _version_ | 1848852244296892416 |
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| author | Wong, Clement Kiing Fook Lai, Kok Song Wong, Mui Yun Mahmood, Maziah |
| author_facet | Wong, Clement Kiing Fook Lai, Kok Song Wong, Mui Yun Mahmood, Maziah |
| author_sort | Wong, Clement Kiing Fook |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Determining the suitable and optimized Minimal Inhibitory Concentration (MIC) of the selectable agents is critical to successfully recover stably transformed plants. In this study, the MIC of five different antibiotics and one herbicide was determined for their inhibitory effect on the growth of a recalcitrant rice cultivar, MR219 using rice shoot apices as target tissue. Antibiotics such as kanamycin, paromomycin and neomycin did not inhibit the shoot apices growth while geneticin (G-418) and hygromycin showed complete growth inhibition at 400 and 25 mg LG1 , respectively. Similarly, the herbicide basta indicated that 9 mg LG1 was sufficient to cause growth inhibition. To conclude, hygromycin and basta were suggested to the suitable selectable agent to select transformed rice shoot apices. |
| first_indexed | 2025-11-15T10:35:00Z |
| format | Article |
| id | upm-53288 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:35:00Z |
| publishDate | 2016 |
| publisher | Science Alert |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-532882022-03-18T02:00:20Z http://psasir.upm.edu.my/id/eprint/53288/ Minimal inhibitory concentration of common selectable agents on recalcitrant Malaysian rice cultivar Wong, Clement Kiing Fook Lai, Kok Song Wong, Mui Yun Mahmood, Maziah Determining the suitable and optimized Minimal Inhibitory Concentration (MIC) of the selectable agents is critical to successfully recover stably transformed plants. In this study, the MIC of five different antibiotics and one herbicide was determined for their inhibitory effect on the growth of a recalcitrant rice cultivar, MR219 using rice shoot apices as target tissue. Antibiotics such as kanamycin, paromomycin and neomycin did not inhibit the shoot apices growth while geneticin (G-418) and hygromycin showed complete growth inhibition at 400 and 25 mg LG1 , respectively. Similarly, the herbicide basta indicated that 9 mg LG1 was sufficient to cause growth inhibition. To conclude, hygromycin and basta were suggested to the suitable selectable agent to select transformed rice shoot apices. Science Alert 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/53288/1/Minimal%20inhibitory%20concentration%20of%20common%20selectable%20agents%20on%20recalcitrant%20Malaysian%20rice%20cultivar.pdf Wong, Clement Kiing Fook and Lai, Kok Song and Wong, Mui Yun and Mahmood, Maziah (2016) Minimal inhibitory concentration of common selectable agents on recalcitrant Malaysian rice cultivar. Asian Journal of Agricultural Research, 10 (2). pp. 126-131. ISSN 1819-1894 https://scialert.net/abstract/?doi=ajar.2016.126.131 10.3923/ajar.2016.126.131 |
| spellingShingle | Wong, Clement Kiing Fook Lai, Kok Song Wong, Mui Yun Mahmood, Maziah Minimal inhibitory concentration of common selectable agents on recalcitrant Malaysian rice cultivar |
| title | Minimal inhibitory concentration of common selectable agents on recalcitrant Malaysian rice cultivar |
| title_full | Minimal inhibitory concentration of common selectable agents on recalcitrant Malaysian rice cultivar |
| title_fullStr | Minimal inhibitory concentration of common selectable agents on recalcitrant Malaysian rice cultivar |
| title_full_unstemmed | Minimal inhibitory concentration of common selectable agents on recalcitrant Malaysian rice cultivar |
| title_short | Minimal inhibitory concentration of common selectable agents on recalcitrant Malaysian rice cultivar |
| title_sort | minimal inhibitory concentration of common selectable agents on recalcitrant malaysian rice cultivar |
| url | http://psasir.upm.edu.my/id/eprint/53288/ http://psasir.upm.edu.my/id/eprint/53288/ http://psasir.upm.edu.my/id/eprint/53288/ http://psasir.upm.edu.my/id/eprint/53288/1/Minimal%20inhibitory%20concentration%20of%20common%20selectable%20agents%20on%20recalcitrant%20Malaysian%20rice%20cultivar.pdf |