Microwave-assisted dilute acid pretreatment and enzymatic hydrolysis of sago palm bark
Maximizing the amount of monomeric sugar yield from lignocellulosic materials requires an effective pretreatment process and identification of an optimal enzyme loading for cost-effectiveness. In this work, a microwave-diluted sulfuric acid pretreatment was applied prior to enzymatic hydrolysis of s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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North Carolina State University
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53284/ http://psasir.upm.edu.my/id/eprint/53284/1/Microwave-assisted%20dilute%20acid%20pretreatment%20and%20enzymatic%20hydrolysis%20of%20sago%20palm%20bark.pdf |
| _version_ | 1848852243192741888 |
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| author | Ethaib, Saleem Omar, Rozita Mustapa Kamal, Siti Mazlina Awang Biak, Dayang Radiah Syafiie, Syams |
| author_facet | Ethaib, Saleem Omar, Rozita Mustapa Kamal, Siti Mazlina Awang Biak, Dayang Radiah Syafiie, Syams |
| author_sort | Ethaib, Saleem |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Maximizing the amount of monomeric sugar yield from lignocellulosic materials requires an effective pretreatment process and identification of an optimal enzyme loading for cost-effectiveness. In this work, a microwave-diluted sulfuric acid pretreatment was applied prior to enzymatic hydrolysis of sago palm bark (SPB). Characterization of the solid fraction was completed before and after the pretreatment process. Analysis of SPB ash showed a presence of 6.8% silica. There was a 32% reduction in lignin content, an increased crystallinity from 29% to 47%, and clear damage and fragmentation to the surface structure of SPB after the pretreatment. Inhibitors were not detectable in the liquor after the microwave-acid pretreatment. The enzymatic hydrolysis of SPB was employed by adding 6 to 42 FPU/g of cellulase and 50 U/g of β-glucosidase to identify the optimal cellulase loading at fixed β-glucosidase loading. The maximum total monomeric sugar yield and total reducing sugar (using DNS method) at 77 mg/g and 378 mg/g were achieved using 24 FPU/g of cellulose, respectively. Thus, this enzyme loading can be recommended for further microwave-acid pretreatment and enzymatic hydrolysis of SPB. |
| first_indexed | 2025-11-15T10:34:59Z |
| format | Article |
| id | upm-53284 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:34:59Z |
| publishDate | 2016 |
| publisher | North Carolina State University |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-532842022-03-18T05:03:29Z http://psasir.upm.edu.my/id/eprint/53284/ Microwave-assisted dilute acid pretreatment and enzymatic hydrolysis of sago palm bark Ethaib, Saleem Omar, Rozita Mustapa Kamal, Siti Mazlina Awang Biak, Dayang Radiah Syafiie, Syams Maximizing the amount of monomeric sugar yield from lignocellulosic materials requires an effective pretreatment process and identification of an optimal enzyme loading for cost-effectiveness. In this work, a microwave-diluted sulfuric acid pretreatment was applied prior to enzymatic hydrolysis of sago palm bark (SPB). Characterization of the solid fraction was completed before and after the pretreatment process. Analysis of SPB ash showed a presence of 6.8% silica. There was a 32% reduction in lignin content, an increased crystallinity from 29% to 47%, and clear damage and fragmentation to the surface structure of SPB after the pretreatment. Inhibitors were not detectable in the liquor after the microwave-acid pretreatment. The enzymatic hydrolysis of SPB was employed by adding 6 to 42 FPU/g of cellulase and 50 U/g of β-glucosidase to identify the optimal cellulase loading at fixed β-glucosidase loading. The maximum total monomeric sugar yield and total reducing sugar (using DNS method) at 77 mg/g and 378 mg/g were achieved using 24 FPU/g of cellulose, respectively. Thus, this enzyme loading can be recommended for further microwave-acid pretreatment and enzymatic hydrolysis of SPB. North Carolina State University 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/53284/1/Microwave-assisted%20dilute%20acid%20pretreatment%20and%20enzymatic%20hydrolysis%20of%20sago%20palm%20bark.pdf Ethaib, Saleem and Omar, Rozita and Mustapa Kamal, Siti Mazlina and Awang Biak, Dayang Radiah and Syafiie, Syams (2016) Microwave-assisted dilute acid pretreatment and enzymatic hydrolysis of sago palm bark. North Carolina State University * College of Natural Resources, 11 (3). pp. 5687-5702. ISSN 1930-2126 https://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_3_5687_Ethaib_Microwave_Dilute_Acid_Pretreatment_Enzymatic_Hydrolysis |
| spellingShingle | Ethaib, Saleem Omar, Rozita Mustapa Kamal, Siti Mazlina Awang Biak, Dayang Radiah Syafiie, Syams Microwave-assisted dilute acid pretreatment and enzymatic hydrolysis of sago palm bark |
| title | Microwave-assisted dilute acid pretreatment and enzymatic hydrolysis of sago palm bark |
| title_full | Microwave-assisted dilute acid pretreatment and enzymatic hydrolysis of sago palm bark |
| title_fullStr | Microwave-assisted dilute acid pretreatment and enzymatic hydrolysis of sago palm bark |
| title_full_unstemmed | Microwave-assisted dilute acid pretreatment and enzymatic hydrolysis of sago palm bark |
| title_short | Microwave-assisted dilute acid pretreatment and enzymatic hydrolysis of sago palm bark |
| title_sort | microwave-assisted dilute acid pretreatment and enzymatic hydrolysis of sago palm bark |
| url | http://psasir.upm.edu.my/id/eprint/53284/ http://psasir.upm.edu.my/id/eprint/53284/ http://psasir.upm.edu.my/id/eprint/53284/1/Microwave-assisted%20dilute%20acid%20pretreatment%20and%20enzymatic%20hydrolysis%20of%20sago%20palm%20bark.pdf |