Microwave-alkali treatment of chicken feathers for protein hydrolysate production
Purpose: Conversion of chicken feathers into valuable products like protein and amino acids is very challenging due to the rigid structure of keratins extensively cross-linked by disulphide, hydrogen and hydrophobic bonds. An efficient treatment is necessary for reducing the disulphide bonds and inc...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer Netherlands
2016
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/53283/ http://psasir.upm.edu.my/id/eprint/53283/1/Microwave-alkali%20treatment%20of%20chicken%20feathers%20for%20protein%20hydrolysate%20production.pdf |
| _version_ | 1848852242941083648 |
|---|---|
| author | Lee, Yen Sze Phang, Lai-Yee Ahmad, Siti Aqlima Ooi, Peck Toung |
| author_facet | Lee, Yen Sze Phang, Lai-Yee Ahmad, Siti Aqlima Ooi, Peck Toung |
| author_sort | Lee, Yen Sze |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Purpose: Conversion of chicken feathers into valuable products like protein and amino acids is very challenging due to the rigid structure of keratins extensively cross-linked by disulphide, hydrogen and hydrophobic bonds. An efficient treatment is necessary for reducing the disulphide bonds and increasing the feathers solubilisation. This study investigated the effects of microwave-alkali treatment on disulphide bond reduction and morphological changes of chicken feathers for protein hydrolysate production. Methods: Feathers were treated at different sodium hydroxide concentrations (0.1, 0.5, 1.0, 1.5, 2.0 M), various microwave power levels (100, 300, 450, 600, 800 W) and residence times (2, 4, 6, 8, 10 min). Results: The most efficient conditions for microwave-alkali treatment of feathers were (1) 10 min; (2) 0.5 M NaOH and (3) 800 W which produced 24.72 mM thiol and 26.74 mg/mL protein. In comparison to the autoclave-alkali and the conventional heating-alkali method, the microwave-alkali treatment denatured the feather keratins and reduced the disulphide bonds in feathers to a greater extent. Scanning electron microscope and fourier transform infrared analyses showed that the structure of the microwave-alkali treated feathers was highly disrupted and significantly changed from fibers into an amorphous structure. Based on the amino acid profile, the protein hydrolysate from the microwave-alkali treatment contained a significantly higher concentration of amino acids (69.4 mg/g of feathers) compared to the autoclave-alkali (19.0 mg/g of feathers) and the conventional heating-alkali (27.8 mg/g of feathers) treatments. Conclusions: Microwave-alkali treatment was more efficient than conventional treatments in breaking down the disulphide bonds, disrupting the feather structure and producing protein hydrolysate. |
| first_indexed | 2025-11-15T10:34:58Z |
| format | Article |
| id | upm-53283 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:34:58Z |
| publishDate | 2016 |
| publisher | Springer Netherlands |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-532832022-03-18T01:52:43Z http://psasir.upm.edu.my/id/eprint/53283/ Microwave-alkali treatment of chicken feathers for protein hydrolysate production Lee, Yen Sze Phang, Lai-Yee Ahmad, Siti Aqlima Ooi, Peck Toung Purpose: Conversion of chicken feathers into valuable products like protein and amino acids is very challenging due to the rigid structure of keratins extensively cross-linked by disulphide, hydrogen and hydrophobic bonds. An efficient treatment is necessary for reducing the disulphide bonds and increasing the feathers solubilisation. This study investigated the effects of microwave-alkali treatment on disulphide bond reduction and morphological changes of chicken feathers for protein hydrolysate production. Methods: Feathers were treated at different sodium hydroxide concentrations (0.1, 0.5, 1.0, 1.5, 2.0 M), various microwave power levels (100, 300, 450, 600, 800 W) and residence times (2, 4, 6, 8, 10 min). Results: The most efficient conditions for microwave-alkali treatment of feathers were (1) 10 min; (2) 0.5 M NaOH and (3) 800 W which produced 24.72 mM thiol and 26.74 mg/mL protein. In comparison to the autoclave-alkali and the conventional heating-alkali method, the microwave-alkali treatment denatured the feather keratins and reduced the disulphide bonds in feathers to a greater extent. Scanning electron microscope and fourier transform infrared analyses showed that the structure of the microwave-alkali treated feathers was highly disrupted and significantly changed from fibers into an amorphous structure. Based on the amino acid profile, the protein hydrolysate from the microwave-alkali treatment contained a significantly higher concentration of amino acids (69.4 mg/g of feathers) compared to the autoclave-alkali (19.0 mg/g of feathers) and the conventional heating-alkali (27.8 mg/g of feathers) treatments. Conclusions: Microwave-alkali treatment was more efficient than conventional treatments in breaking down the disulphide bonds, disrupting the feather structure and producing protein hydrolysate. Springer Netherlands 2016-10 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/53283/1/Microwave-alkali%20treatment%20of%20chicken%20feathers%20for%20protein%20hydrolysate%20production.pdf Lee, Yen Sze and Phang, Lai-Yee and Ahmad, Siti Aqlima and Ooi, Peck Toung (2016) Microwave-alkali treatment of chicken feathers for protein hydrolysate production. Waste and Biomass Valorization, 7. pp. 1147-1157. ISSN 1877-2641; ESSN: 1877-265X https://link.springer.com/article/10.1007/s12649-016-9483-7 10.1007/s12649-016-9483-7 |
| spellingShingle | Lee, Yen Sze Phang, Lai-Yee Ahmad, Siti Aqlima Ooi, Peck Toung Microwave-alkali treatment of chicken feathers for protein hydrolysate production |
| title | Microwave-alkali treatment of chicken feathers for protein hydrolysate production |
| title_full | Microwave-alkali treatment of chicken feathers for protein hydrolysate production |
| title_fullStr | Microwave-alkali treatment of chicken feathers for protein hydrolysate production |
| title_full_unstemmed | Microwave-alkali treatment of chicken feathers for protein hydrolysate production |
| title_short | Microwave-alkali treatment of chicken feathers for protein hydrolysate production |
| title_sort | microwave-alkali treatment of chicken feathers for protein hydrolysate production |
| url | http://psasir.upm.edu.my/id/eprint/53283/ http://psasir.upm.edu.my/id/eprint/53283/ http://psasir.upm.edu.my/id/eprint/53283/ http://psasir.upm.edu.my/id/eprint/53283/1/Microwave-alkali%20treatment%20of%20chicken%20feathers%20for%20protein%20hydrolysate%20production.pdf |