Zerumbone alleviates chronic constriction injury-induced allodynia and hyperalgesia through serotonin 5-HT receptors
Zerumbone, a bioactive sesquiterpene isolated from Zingiber zerumbet (Smith), has shown to exert antiallodynic and antihyperalgesic effects in neuropathic pain mice model in our recent study. The mechanism through which zerumbone alleviates neuropathic pain has yet to be elucidated. Thus, this study...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier Masson
2016
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/53268/ http://psasir.upm.edu.my/id/eprint/53268/1/123.pdf |
| _version_ | 1848852238965932032 |
|---|---|
| author | Chia, Jasmine Siew Min Omar Farouk, Ahmad Akira Mohamad, Azam Shah Sulaiman, Mohd Roslan Perimal, Enoch Kumar |
| author_facet | Chia, Jasmine Siew Min Omar Farouk, Ahmad Akira Mohamad, Azam Shah Sulaiman, Mohd Roslan Perimal, Enoch Kumar |
| author_sort | Chia, Jasmine Siew Min |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Zerumbone, a bioactive sesquiterpene isolated from Zingiber zerumbet (Smith), has shown to exert antiallodynic and antihyperalgesic effects in neuropathic pain mice model in our recent study. The mechanism through which zerumbone alleviates neuropathic pain has yet to be elucidated. Thus, this study aimed to determine whether the serotonergic system, part of the descending pain modulation pathway, contributes to the antineuropathic effect of zerumbone. Participation of the serotonergic system in zerumbone-induced antiallodynia and antihyperalgesia was assessed using Dynamic Plantar Aesthesiometer von Frey test and Hargreaves plantar test respectively in chronic-constriction injury mice model. Administration of ρ-chlorophenylalanine (PCPA, 100 mg/kg, i.p.) for four consecutive days to deplete serotonin (5-HT) prior to zerumbone administration blocked the antiallodynic and antihyperalgesic effects of zerumbone. Further investigation with 5-HT receptor antagonists methiothepin (5-HT 1/6/7 receptor antagonist, 0.1 mg/kg), WAY-100635 (5-HT 1A receptor antagonist, 1 mg/kg), isamoltane (5-HT 1B receptor antagonist, 2.5 mg/kg), ketanserin (5-HT 2A receptor antagonist, 0.3 mg/kg) and ondansetron (5-HT 3 receptor antagonist, 0.5 mg/kg) managed to significantly attenuate antiallodynic and antihyperalgesic effects of zerumbone (10 mg/kg). These findings demonstrate that zerumbone alleviates mechanical allodynia and thermal hyperalgesia through the descending serotonergic system via 5-HT receptors 1A, 1B, 2A, 3, 6 and 7 in chronic constriction injury neuropathic pain mice. |
| first_indexed | 2025-11-15T10:34:55Z |
| format | Article |
| id | upm-53268 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:34:55Z |
| publishDate | 2016 |
| publisher | Elsevier Masson |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-532682017-07-27T05:36:45Z http://psasir.upm.edu.my/id/eprint/53268/ Zerumbone alleviates chronic constriction injury-induced allodynia and hyperalgesia through serotonin 5-HT receptors Chia, Jasmine Siew Min Omar Farouk, Ahmad Akira Mohamad, Azam Shah Sulaiman, Mohd Roslan Perimal, Enoch Kumar Zerumbone, a bioactive sesquiterpene isolated from Zingiber zerumbet (Smith), has shown to exert antiallodynic and antihyperalgesic effects in neuropathic pain mice model in our recent study. The mechanism through which zerumbone alleviates neuropathic pain has yet to be elucidated. Thus, this study aimed to determine whether the serotonergic system, part of the descending pain modulation pathway, contributes to the antineuropathic effect of zerumbone. Participation of the serotonergic system in zerumbone-induced antiallodynia and antihyperalgesia was assessed using Dynamic Plantar Aesthesiometer von Frey test and Hargreaves plantar test respectively in chronic-constriction injury mice model. Administration of ρ-chlorophenylalanine (PCPA, 100 mg/kg, i.p.) for four consecutive days to deplete serotonin (5-HT) prior to zerumbone administration blocked the antiallodynic and antihyperalgesic effects of zerumbone. Further investigation with 5-HT receptor antagonists methiothepin (5-HT 1/6/7 receptor antagonist, 0.1 mg/kg), WAY-100635 (5-HT 1A receptor antagonist, 1 mg/kg), isamoltane (5-HT 1B receptor antagonist, 2.5 mg/kg), ketanserin (5-HT 2A receptor antagonist, 0.3 mg/kg) and ondansetron (5-HT 3 receptor antagonist, 0.5 mg/kg) managed to significantly attenuate antiallodynic and antihyperalgesic effects of zerumbone (10 mg/kg). These findings demonstrate that zerumbone alleviates mechanical allodynia and thermal hyperalgesia through the descending serotonergic system via 5-HT receptors 1A, 1B, 2A, 3, 6 and 7 in chronic constriction injury neuropathic pain mice. Elsevier Masson 2016-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53268/1/123.pdf Chia, Jasmine Siew Min and Omar Farouk, Ahmad Akira and Mohamad, Azam Shah and Sulaiman, Mohd Roslan and Perimal, Enoch Kumar (2016) Zerumbone alleviates chronic constriction injury-induced allodynia and hyperalgesia through serotonin 5-HT receptors. Biomedicine & Pharmacotherapy, 83. pp. 1303-1310. ISSN 0753-3322; ESSN: 1950-6007 10.1016/j.biopha.2016.08.052 |
| spellingShingle | Chia, Jasmine Siew Min Omar Farouk, Ahmad Akira Mohamad, Azam Shah Sulaiman, Mohd Roslan Perimal, Enoch Kumar Zerumbone alleviates chronic constriction injury-induced allodynia and hyperalgesia through serotonin 5-HT receptors |
| title | Zerumbone alleviates chronic constriction injury-induced allodynia and hyperalgesia through serotonin 5-HT receptors |
| title_full | Zerumbone alleviates chronic constriction injury-induced allodynia and hyperalgesia through serotonin 5-HT receptors |
| title_fullStr | Zerumbone alleviates chronic constriction injury-induced allodynia and hyperalgesia through serotonin 5-HT receptors |
| title_full_unstemmed | Zerumbone alleviates chronic constriction injury-induced allodynia and hyperalgesia through serotonin 5-HT receptors |
| title_short | Zerumbone alleviates chronic constriction injury-induced allodynia and hyperalgesia through serotonin 5-HT receptors |
| title_sort | zerumbone alleviates chronic constriction injury-induced allodynia and hyperalgesia through serotonin 5-ht receptors |
| url | http://psasir.upm.edu.my/id/eprint/53268/ http://psasir.upm.edu.my/id/eprint/53268/ http://psasir.upm.edu.my/id/eprint/53268/1/123.pdf |