The effects of different extraction methods on antioxidant properties, chemical composition and thermal behavior of Black Seed (Nigella sativa L.) oil
The Nigella sativa L. popularly referred to as black seeds are widely used as a form of traditional nutrition and medicine. N. sativa seeds were used for the extraction of their oil by way of supercritical fluid extraction (SFE) and cold press (CP) to determine the physicochemical properties, antiox...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/52994/ http://psasir.upm.edu.my/id/eprint/52994/1/The%20effects%20of%20different%20extraction%20methods%20on%20antioxidant%20properties%2C%20chemical%20composition%20and%20thermal%20behavior%20of%20Black%20Seed%20%28Nigella%20sativa%20L.%29%20oil.pdf |
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| author | Mohammed, Nameer Khairullah Abd Manap, Mohd Yazid Tan, Chin Ping Muhialdin, Belal J. Alhelli, Amaal M. Meor Hussin, Anis Shobirin |
| author_facet | Mohammed, Nameer Khairullah Abd Manap, Mohd Yazid Tan, Chin Ping Muhialdin, Belal J. Alhelli, Amaal M. Meor Hussin, Anis Shobirin |
| author_sort | Mohammed, Nameer Khairullah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The Nigella sativa L. popularly referred to as black seeds are widely used as a form of traditional nutrition and medicine. N. sativa seeds were used for the extraction of their oil by way of supercritical fluid extraction (SFE) and cold press (CP) to determine the physicochemical properties, antioxidant activity, and thermal behavior. The GC-MS results showed the primary constituents in the Nigella sativa oil (NSO) were Caryophyllene (17.47%) followed by thymoquinone (TQ) (11.80%), 1,4-Cyclohexadiene (7.17%), longifolene (3.5%), and carvacrol (1.82%). The concentration of TQ was found to be 6.63 mg/mL for oil extracted using SFE and 1.56 mg/mL for oil extracted by CP method. The antioxidant activity measured by DPPH and the IC50 was 1.58 mg/mL and 2.30 mg/mL for SFE oil and cold pressed oil, respectively. The ferric reducing/antioxidant power (FRAP) activity for SFE oil and CP oil was 538.67 mmol/100 mL and 329.00 mmol/100 mL, respectively. The total phenolic content (TPC) of SFE oil was 160.51 mg/100 mL and 94.40 mg/100 mL for CP oil presented as gallic acid equivalents (GAE). This research showed that a high level of natural antioxidants could be derived from NSO extracted by SFE. |
| first_indexed | 2025-11-15T10:34:09Z |
| format | Article |
| id | upm-52994 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:34:09Z |
| publishDate | 2016 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-529942022-05-25T03:28:47Z http://psasir.upm.edu.my/id/eprint/52994/ The effects of different extraction methods on antioxidant properties, chemical composition and thermal behavior of Black Seed (Nigella sativa L.) oil Mohammed, Nameer Khairullah Abd Manap, Mohd Yazid Tan, Chin Ping Muhialdin, Belal J. Alhelli, Amaal M. Meor Hussin, Anis Shobirin The Nigella sativa L. popularly referred to as black seeds are widely used as a form of traditional nutrition and medicine. N. sativa seeds were used for the extraction of their oil by way of supercritical fluid extraction (SFE) and cold press (CP) to determine the physicochemical properties, antioxidant activity, and thermal behavior. The GC-MS results showed the primary constituents in the Nigella sativa oil (NSO) were Caryophyllene (17.47%) followed by thymoquinone (TQ) (11.80%), 1,4-Cyclohexadiene (7.17%), longifolene (3.5%), and carvacrol (1.82%). The concentration of TQ was found to be 6.63 mg/mL for oil extracted using SFE and 1.56 mg/mL for oil extracted by CP method. The antioxidant activity measured by DPPH and the IC50 was 1.58 mg/mL and 2.30 mg/mL for SFE oil and cold pressed oil, respectively. The ferric reducing/antioxidant power (FRAP) activity for SFE oil and CP oil was 538.67 mmol/100 mL and 329.00 mmol/100 mL, respectively. The total phenolic content (TPC) of SFE oil was 160.51 mg/100 mL and 94.40 mg/100 mL for CP oil presented as gallic acid equivalents (GAE). This research showed that a high level of natural antioxidants could be derived from NSO extracted by SFE. Hindawi Publishing Corporation 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/52994/1/The%20effects%20of%20different%20extraction%20methods%20on%20antioxidant%20properties%2C%20chemical%20composition%20and%20thermal%20behavior%20of%20Black%20Seed%20%28Nigella%20sativa%20L.%29%20oil.pdf Mohammed, Nameer Khairullah and Abd Manap, Mohd Yazid and Tan, Chin Ping and Muhialdin, Belal J. and Alhelli, Amaal M. and Meor Hussin, Anis Shobirin (2016) The effects of different extraction methods on antioxidant properties, chemical composition and thermal behavior of Black Seed (Nigella sativa L.) oil. Evidence-Based Complementary and Alternative Medicine, 2016. art. no. 6273817. pp. 1-10. ISSN 1741-427X; ESSN: 1741-4288 https://www.hindawi.com/journals/ecam/2016/6273817/ 10.1155/2016/6273817 |
| spellingShingle | Mohammed, Nameer Khairullah Abd Manap, Mohd Yazid Tan, Chin Ping Muhialdin, Belal J. Alhelli, Amaal M. Meor Hussin, Anis Shobirin The effects of different extraction methods on antioxidant properties, chemical composition and thermal behavior of Black Seed (Nigella sativa L.) oil |
| title | The effects of different extraction methods on antioxidant properties, chemical composition and thermal behavior of Black Seed (Nigella sativa L.) oil |
| title_full | The effects of different extraction methods on antioxidant properties, chemical composition and thermal behavior of Black Seed (Nigella sativa L.) oil |
| title_fullStr | The effects of different extraction methods on antioxidant properties, chemical composition and thermal behavior of Black Seed (Nigella sativa L.) oil |
| title_full_unstemmed | The effects of different extraction methods on antioxidant properties, chemical composition and thermal behavior of Black Seed (Nigella sativa L.) oil |
| title_short | The effects of different extraction methods on antioxidant properties, chemical composition and thermal behavior of Black Seed (Nigella sativa L.) oil |
| title_sort | effects of different extraction methods on antioxidant properties, chemical composition and thermal behavior of black seed (nigella sativa l.) oil |
| url | http://psasir.upm.edu.my/id/eprint/52994/ http://psasir.upm.edu.my/id/eprint/52994/ http://psasir.upm.edu.my/id/eprint/52994/ http://psasir.upm.edu.my/id/eprint/52994/1/The%20effects%20of%20different%20extraction%20methods%20on%20antioxidant%20properties%2C%20chemical%20composition%20and%20thermal%20behavior%20of%20Black%20Seed%20%28Nigella%20sativa%20L.%29%20oil.pdf |