Anomalous reflection of gold: a novel platform for biochips
The importance of protein detection system for protein functions analyses in recent post-genomic era is rising with the emergence of label-free protein detection methods. We are focusing on a simple and practical label-free optical-detection method called anomalous reflection (AR) of gold. When a mo...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Book Section |
| Language: | English |
| Published: |
Humana Press
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/52679/ http://psasir.upm.edu.my/id/eprint/52679/1/Anomalous%20reflection%20of%20gold.pdf |
| _version_ | 1848852150922248192 |
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| author | Amir Hamzah, Amir Syahir Tomizaki, Kin-ya Kajikawa, Kotaro Mihara, Hisakazu |
| author2 | Cretich, Marina |
| author_facet | Cretich, Marina Amir Hamzah, Amir Syahir Tomizaki, Kin-ya Kajikawa, Kotaro Mihara, Hisakazu |
| author_sort | Amir Hamzah, Amir Syahir |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The importance of protein detection system for protein functions analyses in recent post-genomic era is rising with the emergence of label-free protein detection methods. We are focusing on a simple and practical label-free optical-detection method called anomalous reflection (AR) of gold. When a molecular layer forms on the gold surface, significant reduction in reflectivity can be observed at wavelengths of 400–500 nm. This allows the detection of molecular interactions by monitoring changes in reflectivity. In this chapter, we describe the AR method with three different application platforms: (1) gold, (2) gold containing alloy/composite (AuAg2O), and (3) metal-insulator-metal (MIM) thin layers. The AuAg2O composite and MIM are implemented as important concepts for signal enhancement process for the AR technique. Moreover, the observed molecular adsorption and activity is aided by a three-dimensional surface geometry, performed using poly(amidoamine) or PAMAM dendrimer modification. The described system is suitable to be used as a platform for high-throughput detection system in a chip format. |
| first_indexed | 2025-11-15T10:33:31Z |
| format | Book Section |
| id | upm-52679 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:33:31Z |
| publishDate | 2016 |
| publisher | Humana Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-526792021-09-04T23:10:47Z http://psasir.upm.edu.my/id/eprint/52679/ Anomalous reflection of gold: a novel platform for biochips Amir Hamzah, Amir Syahir Tomizaki, Kin-ya Kajikawa, Kotaro Mihara, Hisakazu The importance of protein detection system for protein functions analyses in recent post-genomic era is rising with the emergence of label-free protein detection methods. We are focusing on a simple and practical label-free optical-detection method called anomalous reflection (AR) of gold. When a molecular layer forms on the gold surface, significant reduction in reflectivity can be observed at wavelengths of 400–500 nm. This allows the detection of molecular interactions by monitoring changes in reflectivity. In this chapter, we describe the AR method with three different application platforms: (1) gold, (2) gold containing alloy/composite (AuAg2O), and (3) metal-insulator-metal (MIM) thin layers. The AuAg2O composite and MIM are implemented as important concepts for signal enhancement process for the AR technique. Moreover, the observed molecular adsorption and activity is aided by a three-dimensional surface geometry, performed using poly(amidoamine) or PAMAM dendrimer modification. The described system is suitable to be used as a platform for high-throughput detection system in a chip format. Humana Press Cretich, Marina Chiari, Marcella 2016 Book Section PeerReviewed text en http://psasir.upm.edu.my/id/eprint/52679/1/Anomalous%20reflection%20of%20gold.pdf Amir Hamzah, Amir Syahir and Tomizaki, Kin-ya and Kajikawa, Kotaro and Mihara, Hisakazu (2016) Anomalous reflection of gold: a novel platform for biochips. In: Peptide Microarrays. Methods in Molecular Biology . Humana Press, New York, pp. 97-110. ISBN 9781493930364; EISBN: 9781493930371 https://link.springer.com/protocol/10.1007%2F978-1-4939-3037-1_8 10.1007/978-1-4939-3037-1_8 |
| spellingShingle | Amir Hamzah, Amir Syahir Tomizaki, Kin-ya Kajikawa, Kotaro Mihara, Hisakazu Anomalous reflection of gold: a novel platform for biochips |
| title | Anomalous reflection of gold: a novel platform for biochips |
| title_full | Anomalous reflection of gold: a novel platform for biochips |
| title_fullStr | Anomalous reflection of gold: a novel platform for biochips |
| title_full_unstemmed | Anomalous reflection of gold: a novel platform for biochips |
| title_short | Anomalous reflection of gold: a novel platform for biochips |
| title_sort | anomalous reflection of gold: a novel platform for biochips |
| url | http://psasir.upm.edu.my/id/eprint/52679/ http://psasir.upm.edu.my/id/eprint/52679/ http://psasir.upm.edu.my/id/eprint/52679/ http://psasir.upm.edu.my/id/eprint/52679/1/Anomalous%20reflection%20of%20gold.pdf |