Elucidating the expression of zinc transporters involved in zinc uptake by upland rice landraces in Malaysia
Zinc (Zn) is an essential nutrient that plays important roles in numerous physiological processes in plants, serving as a cofactor for many enzymes and as the key structural motifs in transcriptional regulatory proteins. A deficiency of Zn, therefore, decreases growth, but excess Zn has significant...
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| Format: | Article |
| Language: | English |
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American-Eurasian Network for Scientific Information
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/52482/ http://psasir.upm.edu.my/id/eprint/52482/1/Elucidating%20the%20expression%20of%20zinc%20transporters%20involved%20in%20zinc%20uptake%20by%20upland%20rice%20landraces%20in%20Malaysia.pdf |
| _version_ | 1848852117079457792 |
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| author | Sharifianpour, Golnaz Abdul Rahman, Zaharah Ishak, Che Fauziah Musa, Mohamed Hanafi Nejat, Naghmeh Sahebi, Mahbod Sharifkhani, Ahmad Azizi, Parisa |
| author_facet | Sharifianpour, Golnaz Abdul Rahman, Zaharah Ishak, Che Fauziah Musa, Mohamed Hanafi Nejat, Naghmeh Sahebi, Mahbod Sharifkhani, Ahmad Azizi, Parisa |
| author_sort | Sharifianpour, Golnaz |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Zinc (Zn) is an essential nutrient that plays important roles in numerous physiological processes in plants, serving as a cofactor for many enzymes and as the key structural motifs in transcriptional regulatory proteins. A deficiency of Zn, therefore, decreases growth, but excess Zn has significant toxicity to biological systems through metal-based cytotoxic reactions. Zinc uptake in relation to gene expression was studied by conducting a solution culture experiment. Two upland rice landraces were selected from Malaysia. Germinated seedlings were grown in Yoshida standard solution culture for 2 weeks. Plants were transferred into a modified nutrient culture for a further 2 weeks. (The ZnSO4 was omitted from the solution to induce Zn deficiency). Total RNA were extracted from shoots and roots. Quantitative analysis of 7 genes (OsZIP1, OsZIP3, OsZIP4, OsZIP5, OsZIP8, α-tubulin, and 18srRNA) using commercial primers were carried out by Q-PCR. Results of this study in roots showed over expression of OsZIP4, OsZIP1, OsZIP8 and OsZIP5 under Zn deficiency condition, but the expression of OsZIP3 was not shown. OsZIP4, OsZIP8 and OsZIP5 were expressed in the shoots, while OsZIP1 and OsZIP3 were not up regulated by Zn deficiency (OsZIP3 was not expressed in roots and shoots). OsZIPs were expressed more in the Zn-deficient Bertih than in the Zn-deficient Nabawan variety. |
| first_indexed | 2025-11-15T10:32:58Z |
| format | Article |
| id | upm-52482 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:32:58Z |
| publishDate | 2013 |
| publisher | American-Eurasian Network for Scientific Information |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-524822017-06-06T10:31:56Z http://psasir.upm.edu.my/id/eprint/52482/ Elucidating the expression of zinc transporters involved in zinc uptake by upland rice landraces in Malaysia Sharifianpour, Golnaz Abdul Rahman, Zaharah Ishak, Che Fauziah Musa, Mohamed Hanafi Nejat, Naghmeh Sahebi, Mahbod Sharifkhani, Ahmad Azizi, Parisa Zinc (Zn) is an essential nutrient that plays important roles in numerous physiological processes in plants, serving as a cofactor for many enzymes and as the key structural motifs in transcriptional regulatory proteins. A deficiency of Zn, therefore, decreases growth, but excess Zn has significant toxicity to biological systems through metal-based cytotoxic reactions. Zinc uptake in relation to gene expression was studied by conducting a solution culture experiment. Two upland rice landraces were selected from Malaysia. Germinated seedlings were grown in Yoshida standard solution culture for 2 weeks. Plants were transferred into a modified nutrient culture for a further 2 weeks. (The ZnSO4 was omitted from the solution to induce Zn deficiency). Total RNA were extracted from shoots and roots. Quantitative analysis of 7 genes (OsZIP1, OsZIP3, OsZIP4, OsZIP5, OsZIP8, α-tubulin, and 18srRNA) using commercial primers were carried out by Q-PCR. Results of this study in roots showed over expression of OsZIP4, OsZIP1, OsZIP8 and OsZIP5 under Zn deficiency condition, but the expression of OsZIP3 was not shown. OsZIP4, OsZIP8 and OsZIP5 were expressed in the shoots, while OsZIP1 and OsZIP3 were not up regulated by Zn deficiency (OsZIP3 was not expressed in roots and shoots). OsZIPs were expressed more in the Zn-deficient Bertih than in the Zn-deficient Nabawan variety. American-Eurasian Network for Scientific Information 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/52482/1/Elucidating%20the%20expression%20of%20zinc%20transporters%20involved%20in%20zinc%20uptake%20by%20upland%20rice%20landraces%20in%20Malaysia.pdf Sharifianpour, Golnaz and Abdul Rahman, Zaharah and Ishak, Che Fauziah and Musa, Mohamed Hanafi and Nejat, Naghmeh and Sahebi, Mahbod and Sharifkhani, Ahmad and Azizi, Parisa (2013) Elucidating the expression of zinc transporters involved in zinc uptake by upland rice landraces in Malaysia. Advances in Environmental Biology, 7 (14). pp. 4854-4857. ISSN 1995-0756; ESSN: 1998-1066 http://www.aensiweb.com/old/aeb_December_2013.html |
| spellingShingle | Sharifianpour, Golnaz Abdul Rahman, Zaharah Ishak, Che Fauziah Musa, Mohamed Hanafi Nejat, Naghmeh Sahebi, Mahbod Sharifkhani, Ahmad Azizi, Parisa Elucidating the expression of zinc transporters involved in zinc uptake by upland rice landraces in Malaysia |
| title | Elucidating the expression of zinc transporters involved in zinc uptake by upland rice landraces in Malaysia |
| title_full | Elucidating the expression of zinc transporters involved in zinc uptake by upland rice landraces in Malaysia |
| title_fullStr | Elucidating the expression of zinc transporters involved in zinc uptake by upland rice landraces in Malaysia |
| title_full_unstemmed | Elucidating the expression of zinc transporters involved in zinc uptake by upland rice landraces in Malaysia |
| title_short | Elucidating the expression of zinc transporters involved in zinc uptake by upland rice landraces in Malaysia |
| title_sort | elucidating the expression of zinc transporters involved in zinc uptake by upland rice landraces in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/52482/ http://psasir.upm.edu.my/id/eprint/52482/ http://psasir.upm.edu.my/id/eprint/52482/1/Elucidating%20the%20expression%20of%20zinc%20transporters%20involved%20in%20zinc%20uptake%20by%20upland%20rice%20landraces%20in%20Malaysia.pdf |