Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol
Enhancing of the efficient tissue culture protocol for somatic embryos would facilitate the engineered breeding plants program. In this report, we describe the reproducible protocol of Malaysian rice (Oryza sativa L.) cultivar MR219 through somatic embryogenesis. Effect of a wide spectrum of exogene...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/52454/ http://psasir.upm.edu.my/id/eprint/52454/1/Enhancing%20somatic%20embryogenesis%20of%20Malaysian%20rice%20cultivar%20MR219%20using%20adjuvant%20materials%20in%20a%20high-efficiency%20protocol.pdf |
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| author | Abiri, Rambod Mahmood, Maziah Shaharuddin, Noor Azmi Balia Yusof, Zetty Norhana Atabaki, Narges Musa, Mohamed Hanafi Sahebi, Mahbod Azizi, Parisa Kalhori, Nahid Valdiani, Alireza |
| author_facet | Abiri, Rambod Mahmood, Maziah Shaharuddin, Noor Azmi Balia Yusof, Zetty Norhana Atabaki, Narges Musa, Mohamed Hanafi Sahebi, Mahbod Azizi, Parisa Kalhori, Nahid Valdiani, Alireza |
| author_sort | Abiri, Rambod |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Enhancing of the efficient tissue culture protocol for somatic embryos would facilitate the engineered breeding plants program. In this report, we describe the reproducible protocol of Malaysian rice (Oryza sativa L.) cultivar MR219 through somatic embryogenesis. Effect of a wide spectrum of exogenesis materials was assessed in three phases, namely callogenesis, proliferation and regeneration. Initially, rice seeds were subjected under various auxin treatments. Secondly, the effect of different concentrations of 2,4-D on callus induction was evaluated. In the next step, the efficiency of different explants was identified. Subsequently, the effects of different auxins, cytokinins, l-proline, casein hydrolysate and potassium metasilicate concentrations on the callus proliferation and regeneration were considered. For the callogenesis phase, 2 mg L−1of 2,4-D and roots were chosen as the best auxin and explant. In the callus proliferation stage, the highest efficiency was observed at week eight in the MS media supplemented with 2 mg L−1 of 2,4-D, 2 mg L−1 of kinetin, 50 mg L−1 of l-proline, 100 mg L−1 of casein hydrolysate and 30 mg L−1 of potassium metasilicate. In the last phase of the research, the MS media added with 3 mg L−1 of kinetin, 30 mg L−1of potassium metasilicate and 2 mg L−1 of NAA were selected. Meanwhile, to promote the roots of regenerated explants, 0.4 mg L−1 of IBA has shown potential as an appropriate activator. |
| first_indexed | 2025-11-15T10:32:51Z |
| format | Article |
| id | upm-52454 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:32:51Z |
| publishDate | 2017 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-524542017-06-06T09:11:25Z http://psasir.upm.edu.my/id/eprint/52454/ Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol Abiri, Rambod Mahmood, Maziah Shaharuddin, Noor Azmi Balia Yusof, Zetty Norhana Atabaki, Narges Musa, Mohamed Hanafi Sahebi, Mahbod Azizi, Parisa Kalhori, Nahid Valdiani, Alireza Enhancing of the efficient tissue culture protocol for somatic embryos would facilitate the engineered breeding plants program. In this report, we describe the reproducible protocol of Malaysian rice (Oryza sativa L.) cultivar MR219 through somatic embryogenesis. Effect of a wide spectrum of exogenesis materials was assessed in three phases, namely callogenesis, proliferation and regeneration. Initially, rice seeds were subjected under various auxin treatments. Secondly, the effect of different concentrations of 2,4-D on callus induction was evaluated. In the next step, the efficiency of different explants was identified. Subsequently, the effects of different auxins, cytokinins, l-proline, casein hydrolysate and potassium metasilicate concentrations on the callus proliferation and regeneration were considered. For the callogenesis phase, 2 mg L−1of 2,4-D and roots were chosen as the best auxin and explant. In the callus proliferation stage, the highest efficiency was observed at week eight in the MS media supplemented with 2 mg L−1 of 2,4-D, 2 mg L−1 of kinetin, 50 mg L−1 of l-proline, 100 mg L−1 of casein hydrolysate and 30 mg L−1 of potassium metasilicate. In the last phase of the research, the MS media added with 3 mg L−1 of kinetin, 30 mg L−1of potassium metasilicate and 2 mg L−1 of NAA were selected. Meanwhile, to promote the roots of regenerated explants, 0.4 mg L−1 of IBA has shown potential as an appropriate activator. Springer 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/52454/1/Enhancing%20somatic%20embryogenesis%20of%20Malaysian%20rice%20cultivar%20MR219%20using%20adjuvant%20materials%20in%20a%20high-efficiency%20protocol.pdf Abiri, Rambod and Mahmood, Maziah and Shaharuddin, Noor Azmi and Balia Yusof, Zetty Norhana and Atabaki, Narges and Musa, Mohamed Hanafi and Sahebi, Mahbod and Azizi, Parisa and Kalhori, Nahid and Valdiani, Alireza (2017) Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol. International Journal of Environmental Science and Technology, 14 (5). pp. 1091-1108. ISSN 1735-1472; ESSN: 1735-2630 http://rd.springer.com/article/10.1007/s13762-016-1221-y 10.1007/s13762-016-1221-y |
| spellingShingle | Abiri, Rambod Mahmood, Maziah Shaharuddin, Noor Azmi Balia Yusof, Zetty Norhana Atabaki, Narges Musa, Mohamed Hanafi Sahebi, Mahbod Azizi, Parisa Kalhori, Nahid Valdiani, Alireza Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol |
| title | Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol |
| title_full | Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol |
| title_fullStr | Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol |
| title_full_unstemmed | Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol |
| title_short | Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol |
| title_sort | enhancing somatic embryogenesis of malaysian rice cultivar mr219 using adjuvant materials in a high-efficiency protocol |
| url | http://psasir.upm.edu.my/id/eprint/52454/ http://psasir.upm.edu.my/id/eprint/52454/ http://psasir.upm.edu.my/id/eprint/52454/ http://psasir.upm.edu.my/id/eprint/52454/1/Enhancing%20somatic%20embryogenesis%20of%20Malaysian%20rice%20cultivar%20MR219%20using%20adjuvant%20materials%20in%20a%20high-efficiency%20protocol.pdf |