Genetic divergence and heritability of 42 coloured upland rice genotypes (Oryzasativa) as revealed by microsatellites marker and agro-morphological traits

Coloured rice genotypes have greater nutritious value and consumer demand for these varieties is now greater than ever. The documentation of these genotypes is important for the improvement of the rice plant. In this study, 42 coloured rice genotypes were selected for determination of their genetic...

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Main Authors: Ahmad, Faiz, Musa, Mohamed Hanafi, Hakim, Md. Abdul, Yusop, Mohd Rafii, Arolu, Ibrahim Wasiu, Abdullah, Siti Nor Akmar
Format: Article
Language:English
Published: Public Library of Science 2015
Online Access:http://psasir.upm.edu.my/id/eprint/52452/
http://psasir.upm.edu.my/id/eprint/52452/1/52452.PDF
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author Ahmad, Faiz
Musa, Mohamed Hanafi
Hakim, Md. Abdul
Yusop, Mohd Rafii
Arolu, Ibrahim Wasiu
Abdullah, Siti Nor Akmar
author_facet Ahmad, Faiz
Musa, Mohamed Hanafi
Hakim, Md. Abdul
Yusop, Mohd Rafii
Arolu, Ibrahim Wasiu
Abdullah, Siti Nor Akmar
author_sort Ahmad, Faiz
building UPM Institutional Repository
collection Online Access
description Coloured rice genotypes have greater nutritious value and consumer demand for these varieties is now greater than ever. The documentation of these genotypes is important for the improvement of the rice plant. In this study, 42 coloured rice genotypes were selected for determination of their genetic divergence using 25 simple sequence repeat (SSR) primers and 15 agro-morphological traits. Twenty-one out of the 25 SSR primers showed distinct, reproducible polymorphism. A dendrogram constructed using the SSR primers clustered the 42 coloured rice genotypes into 7 groups. Further, principle component analysis showed 75.28% of total variations were explained by the first - three components. All agro-morphological traits showed significant difference at the (p≤0.05) and (p≤0.01) levels. From the dendrogram constructed using the agro-morphological traits, all the genotypes were clustered into four distinct groups. Pearson's correlation coefficient showed that among the 15 agro-morphological traits, the yield contributing factor had positive correlation with the number of tillers, number of panicles, and panicle length. The heritability of the 15 traits ranged from 17.68 to 99.69%. Yield per plant and harvest index showed the highest value for both heritability and genetic advance. The information on the molecular and agro-morphological traits can be used in rice breeding programmes to improve nutritional value and produce higher yields.
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spelling upm-524522017-06-06T09:08:55Z http://psasir.upm.edu.my/id/eprint/52452/ Genetic divergence and heritability of 42 coloured upland rice genotypes (Oryzasativa) as revealed by microsatellites marker and agro-morphological traits Ahmad, Faiz Musa, Mohamed Hanafi Hakim, Md. Abdul Yusop, Mohd Rafii Arolu, Ibrahim Wasiu Abdullah, Siti Nor Akmar Coloured rice genotypes have greater nutritious value and consumer demand for these varieties is now greater than ever. The documentation of these genotypes is important for the improvement of the rice plant. In this study, 42 coloured rice genotypes were selected for determination of their genetic divergence using 25 simple sequence repeat (SSR) primers and 15 agro-morphological traits. Twenty-one out of the 25 SSR primers showed distinct, reproducible polymorphism. A dendrogram constructed using the SSR primers clustered the 42 coloured rice genotypes into 7 groups. Further, principle component analysis showed 75.28% of total variations were explained by the first - three components. All agro-morphological traits showed significant difference at the (p≤0.05) and (p≤0.01) levels. From the dendrogram constructed using the agro-morphological traits, all the genotypes were clustered into four distinct groups. Pearson's correlation coefficient showed that among the 15 agro-morphological traits, the yield contributing factor had positive correlation with the number of tillers, number of panicles, and panicle length. The heritability of the 15 traits ranged from 17.68 to 99.69%. Yield per plant and harvest index showed the highest value for both heritability and genetic advance. The information on the molecular and agro-morphological traits can be used in rice breeding programmes to improve nutritional value and produce higher yields. Public Library of Science 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/52452/1/52452.PDF Ahmad, Faiz and Musa, Mohamed Hanafi and Hakim, Md. Abdul and Yusop, Mohd Rafii and Arolu, Ibrahim Wasiu and Abdullah, Siti Nor Akmar (2015) Genetic divergence and heritability of 42 coloured upland rice genotypes (Oryzasativa) as revealed by microsatellites marker and agro-morphological traits. PLOS ONE, 10 (9). art. no. e0138246. pp. 1-18. ISSN 1932-6203 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0138246 10.1371/journal.pone.0138246
spellingShingle Ahmad, Faiz
Musa, Mohamed Hanafi
Hakim, Md. Abdul
Yusop, Mohd Rafii
Arolu, Ibrahim Wasiu
Abdullah, Siti Nor Akmar
Genetic divergence and heritability of 42 coloured upland rice genotypes (Oryzasativa) as revealed by microsatellites marker and agro-morphological traits
title Genetic divergence and heritability of 42 coloured upland rice genotypes (Oryzasativa) as revealed by microsatellites marker and agro-morphological traits
title_full Genetic divergence and heritability of 42 coloured upland rice genotypes (Oryzasativa) as revealed by microsatellites marker and agro-morphological traits
title_fullStr Genetic divergence and heritability of 42 coloured upland rice genotypes (Oryzasativa) as revealed by microsatellites marker and agro-morphological traits
title_full_unstemmed Genetic divergence and heritability of 42 coloured upland rice genotypes (Oryzasativa) as revealed by microsatellites marker and agro-morphological traits
title_short Genetic divergence and heritability of 42 coloured upland rice genotypes (Oryzasativa) as revealed by microsatellites marker and agro-morphological traits
title_sort genetic divergence and heritability of 42 coloured upland rice genotypes (oryzasativa) as revealed by microsatellites marker and agro-morphological traits
url http://psasir.upm.edu.my/id/eprint/52452/
http://psasir.upm.edu.my/id/eprint/52452/
http://psasir.upm.edu.my/id/eprint/52452/
http://psasir.upm.edu.my/id/eprint/52452/1/52452.PDF