Evaluation of RNA extraction methods in rice and their application in expression analysis of resistance genes against Magnaporthe oryzae
Extraction of RNA of high quality and integrity is essential for gene expression studies and all downstream RNA-based techniques. The leaves of 16 merit Malaysian rice varieties were used to isolate total RNA using five different methods. The quantity, quality and integrity of extracted RNA were con...
| Main Authors: | Azizi, Parisa, Yusop, Mohd Rafii, Mahmood, Maziah, Abdullah, Siti Nor Akmar, Musa, Mohamed Hanafi, Abdul Latif, Muhammad, Sahebi, Mahbod, Ashkani, Sadegh |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis
2017
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/52423/ http://psasir.upm.edu.my/id/eprint/52423/1/Evaluation%20of%20RNA%20extraction%20methods%20in%20rice%20and%20their%20application%20in%20expression%20analysis%20of%20resistance%20genes%20against%20Magnaporthe%20oryzae.pdf |
Similar Items
Toward understanding of rice innate immunity against Magnaporthe oryzae
by: Azizi, Parisa, et al.
Published: (2016)
by: Azizi, Parisa, et al.
Published: (2016)
Overexpression of a gene encoding LRR protein improves rice resistance against Magnaporthe oryzae
by: Azizi, Parisa, et al.
Published: (2017)
by: Azizi, Parisa, et al.
Published: (2017)
Differential gene expression reflects morphological characteristics and physiological processes in rice immunity against blast pathogen Magnaporthe oryzae
by: Azizi, Parisa, et al.
Published: (2015)
by: Azizi, Parisa, et al.
Published: (2015)
Over-expression of the Pikh gene with a CaMV 35S promoter leads to improved blast disease (Magnaporthe oryzae) tolerance in rice
by: Azizi, Parisa, et al.
Published: (2016)
by: Azizi, Parisa, et al.
Published: (2016)
Towards understanding pre-mRNA splicing mechanisms and the role of SR proteins
by: Sahebi, Mahbod, et al.
Published: (2016)
by: Sahebi, Mahbod, et al.
Published: (2016)
Adaptation of the metabolomics profile of rice after Pyricularia oryzae infection
by: Musa, Mohamed Hanafi, et al.
Published: (2019)
by: Musa, Mohamed Hanafi, et al.
Published: (2019)
Isolation and Characterization of Avirulence Genes in Magnaporthe oryzae
by: Mui, Sie Jee, et al.
Published: (2017)
by: Mui, Sie Jee, et al.
Published: (2017)
Allele mining strategies: principles and utilisation for blast resistance genes in rice (Oryza sativa L.)
by: Ashkani, Sadegh, et al.
Published: (2015)
by: Ashkani, Sadegh, et al.
Published: (2015)
Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia
by: Abed-Ashtiani, Farnaz, et al.
Published: (2016)
by: Abed-Ashtiani, Farnaz, et al.
Published: (2016)
Suppression subtractive hybridization versus next-generation sequencing in plant genetic engineering: challenges and perspectives
by: Sahebi, Mahbod, et al.
Published: (2015)
by: Sahebi, Mahbod, et al.
Published: (2015)
Genetic analysis of resistance to rice blast: a study on the inheritance of resistance to the blast disease pathogen in an F3 population of rice
by: Ashkani, Sadegh, et al.
Published: (2015)
by: Ashkani, Sadegh, et al.
Published: (2015)
The effect of magnaporthe oryzae on selected Malaysian rice varieties / Izuwan Mustafa Kamal
by: Mustafa Kamal, Izuwan
Published: (2016)
by: Mustafa Kamal, Izuwan
Published: (2016)
Highly efficient protocol for callogenesis, somagenesis and regeneration of Indica rice plants
by: Azizi, Parisa, et al.
Published: (2015)
by: Azizi, Parisa, et al.
Published: (2015)
SSRs for marker assisted selection for blast resistance in rice (Oryza sativa L.).
by: Ashkani, Sadegh, et al.
Published: (2012)
by: Ashkani, Sadegh, et al.
Published: (2012)
FAR1 and FAR2 regulate the expression of genes associated with lipid metabolism in the rice blast fungus Magnaporthe oryzae
by: Yusof, Mohammad Termizi, et al.
Published: (2014)
by: Yusof, Mohammad Termizi, et al.
Published: (2014)
Mapping of QTLs conferring resistance in rice to brown planthopper, Nilaparvata lugens
by: Shabanimofrad, Mahmoodreza, et al.
Published: (2017)
by: Shabanimofrad, Mahmoodreza, et al.
Published: (2017)
Screening and expression of a silicon transporter gene (Lsi1) in wild-type indica rice cultivars
by: Sahebi, Mahbod, et al.
Published: (2017)
by: Sahebi, Mahbod, et al.
Published: (2017)
Isolation and expression analysis of novel silicon absorption gene from roots of mangrove (Rhizophora apiculata) via suppression subtractive hybridization
by: Sahebi, Mahbod, et al.
Published: (2014)
by: Sahebi, Mahbod, et al.
Published: (2014)
Investigation of metabolites produced by rice blast fungus, Magnaporthe oryzae during appressorium development
by: Md Zain, Azian
Published: (2020)
by: Md Zain, Azian
Published: (2020)
Application of silicon in plant tissue culture
by: Sahebi, Mahbod, et al.
Published: (2016)
by: Sahebi, Mahbod, et al.
Published: (2016)
Understanding the shoot apical meristem regulation: a study of the phytohormones, auxin and cytokinin, in rice
by: Azizi, Parisa, et al.
Published: (2015)
by: Azizi, Parisa, et al.
Published: (2015)
Cloning and sequence analysis of Avr-Pik gene from Magnaporthe oryzae isolates from Sarawak
by: Martina Azelin, Dirum
Published: (2016)
by: Martina Azelin, Dirum
Published: (2016)
Extraction of total RNA and identification of different genes in mangrove plant involved in its adaptability to the variety of stresses
by: Sahebi, Mahbob, et al.
Published: (2013)
by: Sahebi, Mahbob, et al.
Published: (2013)
Analysis of SSR markers linked with brown planthopper resistance genes (Bph) using high-resolution melting (HRM) in rice
by: Shabanimofrad, Mahmoodreza, et al.
Published: (2015)
by: Shabanimofrad, Mahmoodreza, et al.
Published: (2015)
Serine-rich protein is a novel positive regulator for silicon accumulation in mangrove
by: Sahebi, Mahbod, et al.
Published: (2015)
by: Sahebi, Mahbod, et al.
Published: (2015)
Biocontrol characteristics of wild basidiomycetes and their applicability to suppress rice blast fungus, Magnaporthe oryzae / Adil Fikri Jumiry
by: Jumiry, Adil Fikri
Published: (2018)
by: Jumiry, Adil Fikri
Published: (2018)
Three isolate of actinomycetes as biological control against Magnaporthe orzyae and Fusarium solani
by: Aisyah Fatiha Zailan,, et al.
Published: (2024)
by: Aisyah Fatiha Zailan,, et al.
Published: (2024)
Molecular progress on the mapping and cloning of functional genes for blast disease in rice (Oryza sativa L.): current status and future considerations
by: Ashkani, Sadegh, et al.
Published: (2016)
by: Ashkani, Sadegh, et al.
Published: (2016)
Improvement of drought tolerance in rice (Oryza sativa L.): genetics, genomic tools, and the WRKY gene family
by: Sahebi, Mahbod, et al.
Published: (2018)
by: Sahebi, Mahbod, et al.
Published: (2018)
Reactions and diversity analysis of upland rice genotypes against blast disease of rice (Oryza sativa L.)
by: Khatun, Mst. Tuhina, et al.
Published: (2015)
by: Khatun, Mst. Tuhina, et al.
Published: (2015)
An enigma in the genetic responses of plants to salt stresses
by: Azizi, Parisa, et al.
Published: (2019)
by: Azizi, Parisa, et al.
Published: (2019)
Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa).
by: S., Ashkani, et al.
Published: (2011)
by: S., Ashkani, et al.
Published: (2011)
Suppression subtractive hybridization technique in wheat for the identification of disease resistance differentially expressed genes
by: Uzzaman, Md. Aktar, et al.
Published: (2016)
by: Uzzaman, Md. Aktar, et al.
Published: (2016)
Towards immunity of oil palm against Ganoderma fungus infection
by: Sahebi, Mahbod, et al.
Published: (2015)
by: Sahebi, Mahbod, et al.
Published: (2015)
Efficacy of fungicides against grain spot disease in rice (Oryza sativa)
by: Akter, Shamima, et al.
Published: (2013)
by: Akter, Shamima, et al.
Published: (2013)
Genetic divergence and heritability of 42 coloured upland rice genotypes (Oryza sativa) as revealed by microsatellites marker and agro-morphological traits
by: Musa, Mohamed Hanafi, et al.
Published: (2015)
by: Musa, Mohamed Hanafi, et al.
Published: (2015)
Towards immunity of oil palm against Ganoderma fungus infection
by: Sahebi, Mahbod, et al.
Published: (2015)
by: Sahebi, Mahbod, et al.
Published: (2015)
Elucidating the expression of zinc transporters involved in zinc uptake by upland rice landraces in Malaysia
by: Sharifianpour, Golnaz, et al.
Published: (2013)
by: Sharifianpour, Golnaz, et al.
Published: (2013)
Genetic dissection of rice blast resistance by QTL mapping approach using an F3 population
by: Ashkani, Sadegh, et al.
Published: (2013)
by: Ashkani, Sadegh, et al.
Published: (2013)
Mapping of the quantitative trait locus (QTL) conferring partial resistance to rice leaf blast disease
by: Ashkani, Sadegh, et al.
Published: (2013)
by: Ashkani, Sadegh, et al.
Published: (2013)
Similar Items
-
Toward understanding of rice innate immunity against Magnaporthe oryzae
by: Azizi, Parisa, et al.
Published: (2016) -
Overexpression of a gene encoding LRR protein improves rice resistance against Magnaporthe oryzae
by: Azizi, Parisa, et al.
Published: (2017) -
Differential gene expression reflects morphological characteristics and physiological processes in rice immunity against blast pathogen Magnaporthe oryzae
by: Azizi, Parisa, et al.
Published: (2015) -
Over-expression of the Pikh gene with a CaMV 35S promoter leads to improved blast disease (Magnaporthe oryzae) tolerance in rice
by: Azizi, Parisa, et al.
Published: (2016) -
Towards understanding pre-mRNA splicing mechanisms and the role of SR proteins
by: Sahebi, Mahbod, et al.
Published: (2016)