Heavy metals biomonitoring via inhibitive assay of acetylcholinesterase from Periophthalmodon schlosseri
Acetylcholinesterase (AChE) generally known to be sensitive toward insecticides but its sensitivity toward heavy metals was least reported. Herein, a sensitive assay for heavy metals has been pursued using AChE in a rapid and economic manner. The AChE from a mudskipper, Periophthalmodon schlosseri h...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/52092/ http://psasir.upm.edu.my/id/eprint/52092/1/Heavy%20metals%20biomonitoring%20via%20inhibitive%20assay%20of%20acetylcholinesterase%20from%20Periophthalmodon%20schlosseri.pdf |
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| author | Sabullah, Mohd Khalizan Sulaiman, Mohd Rosni Abd Shukor, Mohd Shukri Yusof, Mohd Termizi Wan Johari, Wan Lutfi Abd. Shukor, Mohd. Yunus Amir Hamzah, Amir Syahir |
| author_facet | Sabullah, Mohd Khalizan Sulaiman, Mohd Rosni Abd Shukor, Mohd Shukri Yusof, Mohd Termizi Wan Johari, Wan Lutfi Abd. Shukor, Mohd. Yunus Amir Hamzah, Amir Syahir |
| author_sort | Sabullah, Mohd Khalizan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Acetylcholinesterase (AChE) generally known to be sensitive toward insecticides but its sensitivity toward heavy metals was least reported. Herein, a sensitive assay for heavy metals has been pursued using AChE in a rapid and economic manner. The AChE from a mudskipper, Periophthalmodon schlosseri has been found to be sensitive toward copper > mercury > chromium > and arsenic ions at the sub parts per million levels. Field trial works showed that the assay was applicable in detecting heavy metals pollution from effluents of industrial sites at near real time and verified using ICP-OES and Flow Injection Mercury System (FIMS 400). Furthermore, hierarchical cluster analyses of inhibition profiles were performed, revealing a comparable capability of the AChE compared to the gold standard of Microtox™ method. |
| first_indexed | 2025-11-15T10:31:17Z |
| format | Article |
| id | upm-52092 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:31:17Z |
| publishDate | 2015 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-520922017-05-05T02:55:12Z http://psasir.upm.edu.my/id/eprint/52092/ Heavy metals biomonitoring via inhibitive assay of acetylcholinesterase from Periophthalmodon schlosseri Sabullah, Mohd Khalizan Sulaiman, Mohd Rosni Abd Shukor, Mohd Shukri Yusof, Mohd Termizi Wan Johari, Wan Lutfi Abd. Shukor, Mohd. Yunus Amir Hamzah, Amir Syahir Acetylcholinesterase (AChE) generally known to be sensitive toward insecticides but its sensitivity toward heavy metals was least reported. Herein, a sensitive assay for heavy metals has been pursued using AChE in a rapid and economic manner. The AChE from a mudskipper, Periophthalmodon schlosseri has been found to be sensitive toward copper > mercury > chromium > and arsenic ions at the sub parts per million levels. Field trial works showed that the assay was applicable in detecting heavy metals pollution from effluents of industrial sites at near real time and verified using ICP-OES and Flow Injection Mercury System (FIMS 400). Furthermore, hierarchical cluster analyses of inhibition profiles were performed, revealing a comparable capability of the AChE compared to the gold standard of Microtox™ method. Springer 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/52092/1/Heavy%20metals%20biomonitoring%20via%20inhibitive%20assay%20of%20acetylcholinesterase%20from%20Periophthalmodon%20schlosseri.pdf Sabullah, Mohd Khalizan and Sulaiman, Mohd Rosni and Abd Shukor, Mohd Shukri and Yusof, Mohd Termizi and Wan Johari, Wan Lutfi and Abd. Shukor, Mohd. Yunus and Amir Hamzah, Amir Syahir (2015) Heavy metals biomonitoring via inhibitive assay of acetylcholinesterase from Periophthalmodon schlosseri. Rendiconti Lincei, 26 (2). pp. 151-158. ISSN 2037-4631; ESSN: 1720-0776 http://rd.springer.com/article/10.1007/s12210-014-0359-0 10.1007/s12210-014-0359-0 |
| spellingShingle | Sabullah, Mohd Khalizan Sulaiman, Mohd Rosni Abd Shukor, Mohd Shukri Yusof, Mohd Termizi Wan Johari, Wan Lutfi Abd. Shukor, Mohd. Yunus Amir Hamzah, Amir Syahir Heavy metals biomonitoring via inhibitive assay of acetylcholinesterase from Periophthalmodon schlosseri |
| title | Heavy metals biomonitoring via inhibitive assay of acetylcholinesterase from Periophthalmodon schlosseri |
| title_full | Heavy metals biomonitoring via inhibitive assay of acetylcholinesterase from Periophthalmodon schlosseri |
| title_fullStr | Heavy metals biomonitoring via inhibitive assay of acetylcholinesterase from Periophthalmodon schlosseri |
| title_full_unstemmed | Heavy metals biomonitoring via inhibitive assay of acetylcholinesterase from Periophthalmodon schlosseri |
| title_short | Heavy metals biomonitoring via inhibitive assay of acetylcholinesterase from Periophthalmodon schlosseri |
| title_sort | heavy metals biomonitoring via inhibitive assay of acetylcholinesterase from periophthalmodon schlosseri |
| url | http://psasir.upm.edu.my/id/eprint/52092/ http://psasir.upm.edu.my/id/eprint/52092/ http://psasir.upm.edu.my/id/eprint/52092/ http://psasir.upm.edu.my/id/eprint/52092/1/Heavy%20metals%20biomonitoring%20via%20inhibitive%20assay%20of%20acetylcholinesterase%20from%20Periophthalmodon%20schlosseri.pdf |