Production of cellulase by a wild strain of Chaetomium globosum using delignified oil palm empty-fruit-bunch fibre as substrate
Studies on the feasibility of using delignified oil palm empty-fruit- bunch (OPEFB) fibres as a substrate for cellulase production by Chaetomium globosum strain 414 were carried out in shake-flask cultures containing different types and concentrations of nitrogen source. Peptone, as nitrogen source,...
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| Format: | Article |
| Language: | English English |
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Springer-Verlag
1997
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| Online Access: | http://psasir.upm.edu.my/id/eprint/52077/ http://psasir.upm.edu.my/id/eprint/52077/1/Production%20of%20cellulase%20by%20a%20wild%20strain%20of%20Chaetomium%20globosum%20using%20delignified%20oil%20palm%20empty-fruit-bunch%20fibre%20as%20substrate.pdf http://psasir.upm.edu.my/id/eprint/52077/7/s002530050978.pdf |
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| author | Md. Shah, Umi Kalsom Ariff, Arbakariya Shamsuddin, Zulkifli Tong, C. C. Hassan, Mohd Ali Abdul Karim, Mohamed Ismail |
| author_facet | Md. Shah, Umi Kalsom Ariff, Arbakariya Shamsuddin, Zulkifli Tong, C. C. Hassan, Mohd Ali Abdul Karim, Mohamed Ismail |
| author_sort | Md. Shah, Umi Kalsom |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Studies on the feasibility of using delignified oil palm empty-fruit- bunch (OPEFB) fibres as a substrate for cellulase production by Chaetomium globosum strain 414 were carried out in shake-flask cultures containing different types and concentrations of nitrogen source. Peptone, as nitrogen source, gave maximum production of all the three main components of the cellulase complex (endoglucanase or carboxymethylcellulase, cellobiohydrolase or filter-paper-hydrolysing enzyme and β-glucosidase), followed by yeast extract, urea, KNO3 and (NH4)2SO4. The maximum specific growth rate (μ(max)) of C globosum strain 414 grown in medium containing OPEFB and peptone was 0.038 h-1. In all the fermentations, the fungus was able to produce all the three cellulases with significant amounts of β-glucosidase, except when using (NH4)2SO4 as nitrogen source, where β-glucosidase was not produced. With 6 g/l peptone and 10 g/l delignified OPEFB fibres, the fungus produced maximum concentrations of FPase, carboxymethylcellulase and β-glucosidase: 1.4. 30.8 and 9.8 U/ml, giving productivities of 10, 214 and 24 U l-1 h-1 respectively. The cellulase mixture, partially purified by ammonium sulphate precipitation, was able to hydrolyse delignified OPEFB fibres, converting about 68% of the cellulosies to reducing sugars after 5 days. |
| first_indexed | 2025-11-15T10:31:13Z |
| format | Article |
| id | upm-52077 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T10:31:13Z |
| publishDate | 1997 |
| publisher | Springer-Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-520772024-08-02T06:39:47Z http://psasir.upm.edu.my/id/eprint/52077/ Production of cellulase by a wild strain of Chaetomium globosum using delignified oil palm empty-fruit-bunch fibre as substrate Md. Shah, Umi Kalsom Ariff, Arbakariya Shamsuddin, Zulkifli Tong, C. C. Hassan, Mohd Ali Abdul Karim, Mohamed Ismail Studies on the feasibility of using delignified oil palm empty-fruit- bunch (OPEFB) fibres as a substrate for cellulase production by Chaetomium globosum strain 414 were carried out in shake-flask cultures containing different types and concentrations of nitrogen source. Peptone, as nitrogen source, gave maximum production of all the three main components of the cellulase complex (endoglucanase or carboxymethylcellulase, cellobiohydrolase or filter-paper-hydrolysing enzyme and β-glucosidase), followed by yeast extract, urea, KNO3 and (NH4)2SO4. The maximum specific growth rate (μ(max)) of C globosum strain 414 grown in medium containing OPEFB and peptone was 0.038 h-1. In all the fermentations, the fungus was able to produce all the three cellulases with significant amounts of β-glucosidase, except when using (NH4)2SO4 as nitrogen source, where β-glucosidase was not produced. With 6 g/l peptone and 10 g/l delignified OPEFB fibres, the fungus produced maximum concentrations of FPase, carboxymethylcellulase and β-glucosidase: 1.4. 30.8 and 9.8 U/ml, giving productivities of 10, 214 and 24 U l-1 h-1 respectively. The cellulase mixture, partially purified by ammonium sulphate precipitation, was able to hydrolyse delignified OPEFB fibres, converting about 68% of the cellulosies to reducing sugars after 5 days. Springer-Verlag 1997 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/52077/1/Production%20of%20cellulase%20by%20a%20wild%20strain%20of%20Chaetomium%20globosum%20using%20delignified%20oil%20palm%20empty-fruit-bunch%20fibre%20as%20substrate.pdf text en http://psasir.upm.edu.my/id/eprint/52077/7/s002530050978.pdf Md. Shah, Umi Kalsom and Ariff, Arbakariya and Shamsuddin, Zulkifli and Tong, C. C. and Hassan, Mohd Ali and Abdul Karim, Mohamed Ismail (1997) Production of cellulase by a wild strain of Chaetomium globosum using delignified oil palm empty-fruit-bunch fibre as substrate. Applied Microbiology and Biotechnology, 47 (5). pp. 590-595. ISSN 0175-7598 http://rd.springer.com/article/10.1007/s002530050978 10.1007/s002530050978 |
| spellingShingle | Md. Shah, Umi Kalsom Ariff, Arbakariya Shamsuddin, Zulkifli Tong, C. C. Hassan, Mohd Ali Abdul Karim, Mohamed Ismail Production of cellulase by a wild strain of Chaetomium globosum using delignified oil palm empty-fruit-bunch fibre as substrate |
| title | Production of cellulase by a wild strain of Chaetomium globosum using delignified oil palm empty-fruit-bunch fibre as substrate |
| title_full | Production of cellulase by a wild strain of Chaetomium globosum using delignified oil palm empty-fruit-bunch fibre as substrate |
| title_fullStr | Production of cellulase by a wild strain of Chaetomium globosum using delignified oil palm empty-fruit-bunch fibre as substrate |
| title_full_unstemmed | Production of cellulase by a wild strain of Chaetomium globosum using delignified oil palm empty-fruit-bunch fibre as substrate |
| title_short | Production of cellulase by a wild strain of Chaetomium globosum using delignified oil palm empty-fruit-bunch fibre as substrate |
| title_sort | production of cellulase by a wild strain of chaetomium globosum using delignified oil palm empty-fruit-bunch fibre as substrate |
| url | http://psasir.upm.edu.my/id/eprint/52077/ http://psasir.upm.edu.my/id/eprint/52077/ http://psasir.upm.edu.my/id/eprint/52077/ http://psasir.upm.edu.my/id/eprint/52077/1/Production%20of%20cellulase%20by%20a%20wild%20strain%20of%20Chaetomium%20globosum%20using%20delignified%20oil%20palm%20empty-fruit-bunch%20fibre%20as%20substrate.pdf http://psasir.upm.edu.my/id/eprint/52077/7/s002530050978.pdf |