Papaya (Carica papaya) leaf methanolic extract modulates in vitro rumen methanogenesis and rumen biohydrogenation
Papaya leaf methanolic extract (PLE) at concentrations of 0 (CON), 5 (LLE), 10 (MLE) and 15 (HLE) mg/250 mg dry matter (DM) with 30 mL buffered rumen fluid were incubated for 24 h to identify its effect on in vitro ruminal methanogenesis and ruminal biohydrogenation (BH). Total gas production was no...
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| Format: | Article |
| Language: | English |
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Japanese Society of Animal Science
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/52023/ http://psasir.upm.edu.my/id/eprint/52023/1/Papaya%20%28Carica%20papaya%29%20leaf%20methanolic%20extract%20modulates%20in%20vitro%20rumen%20methanogenesis%20and%20rumen%20biohydrogenation.pdf |
| _version_ | 1848851992723587072 |
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| author | Jafari, Saeid Goh, Yong Meng Rajion, Mohamed Ali Jahromi, Mohammad Faseleh Ahmad, Yusof Ebrahimi, Mahdi |
| author_facet | Jafari, Saeid Goh, Yong Meng Rajion, Mohamed Ali Jahromi, Mohammad Faseleh Ahmad, Yusof Ebrahimi, Mahdi |
| author_sort | Jafari, Saeid |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Papaya leaf methanolic extract (PLE) at concentrations of 0 (CON), 5 (LLE), 10 (MLE) and 15 (HLE) mg/250 mg dry matter (DM) with 30 mL buffered rumen fluid were incubated for 24 h to identify its effect on in vitro ruminal methanogenesis and ruminal biohydrogenation (BH). Total gas production was not affected (P > 0.05) by addition of PLE compared to the CON at 24 h of incubation. Methane (CH4) production (mL/250 mg DM) decreased (P < 0.05) with increasing levels of PLE. Acetate to propionate ratio was lower (P <0.05) in MLE (2.02) and HLE (1.93) compared to the CON (2.28). Supplementation of the diet with PLE significantly (P <0.05) decreased the rate of BH of C18:1n-9 (oleic acid; OA), C18:2n-6 (linoleic acid; LA), C18:3n-3 (linolenic acid; LNA) and C18 polyunsaturated fatty acids (PUFA) compared to CON after 24 h incubation, which resulted in higher concentrations of BH intermediates such as C18:1 t11 (vaccenic acid; VA), c9t11 conjugated LA (CLA) (rumenic acid; RA) and t10c12 CLA. Real-time PCR analysis indicated that the total bacteria, total protozoa, Butyrivibrio fibrisolvens and methanogen population in HLE decreased (P <0.05) compared to CON, but the total bacteria and B. fibrisolvens population were higher (P < 0.05) in CON compared to the PLE treatment groups. |
| first_indexed | 2025-11-15T10:31:00Z |
| format | Article |
| id | upm-52023 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:31:00Z |
| publishDate | 2017 |
| publisher | Japanese Society of Animal Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-520232017-05-04T09:00:31Z http://psasir.upm.edu.my/id/eprint/52023/ Papaya (Carica papaya) leaf methanolic extract modulates in vitro rumen methanogenesis and rumen biohydrogenation Jafari, Saeid Goh, Yong Meng Rajion, Mohamed Ali Jahromi, Mohammad Faseleh Ahmad, Yusof Ebrahimi, Mahdi Papaya leaf methanolic extract (PLE) at concentrations of 0 (CON), 5 (LLE), 10 (MLE) and 15 (HLE) mg/250 mg dry matter (DM) with 30 mL buffered rumen fluid were incubated for 24 h to identify its effect on in vitro ruminal methanogenesis and ruminal biohydrogenation (BH). Total gas production was not affected (P > 0.05) by addition of PLE compared to the CON at 24 h of incubation. Methane (CH4) production (mL/250 mg DM) decreased (P < 0.05) with increasing levels of PLE. Acetate to propionate ratio was lower (P <0.05) in MLE (2.02) and HLE (1.93) compared to the CON (2.28). Supplementation of the diet with PLE significantly (P <0.05) decreased the rate of BH of C18:1n-9 (oleic acid; OA), C18:2n-6 (linoleic acid; LA), C18:3n-3 (linolenic acid; LNA) and C18 polyunsaturated fatty acids (PUFA) compared to CON after 24 h incubation, which resulted in higher concentrations of BH intermediates such as C18:1 t11 (vaccenic acid; VA), c9t11 conjugated LA (CLA) (rumenic acid; RA) and t10c12 CLA. Real-time PCR analysis indicated that the total bacteria, total protozoa, Butyrivibrio fibrisolvens and methanogen population in HLE decreased (P <0.05) compared to CON, but the total bacteria and B. fibrisolvens population were higher (P < 0.05) in CON compared to the PLE treatment groups. Japanese Society of Animal Science 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/52023/1/Papaya%20%28Carica%20papaya%29%20leaf%20methanolic%20extract%20modulates%20in%20vitro%20rumen%20methanogenesis%20and%20rumen%20biohydrogenation.pdf Jafari, Saeid and Goh, Yong Meng and Rajion, Mohamed Ali and Jahromi, Mohammad Faseleh and Ahmad, Yusof and Ebrahimi, Mahdi (2017) Papaya (Carica papaya) leaf methanolic extract modulates in vitro rumen methanogenesis and rumen biohydrogenation. Animal Science Journal, 88 (2). pp. 267-276. ISSN 1344-3941; ESSN: 1740-0929 http://onlinelibrary.wiley.com/wol1/doi/10.1111/asj.12634/abstract 10.1111/asj.12634 |
| spellingShingle | Jafari, Saeid Goh, Yong Meng Rajion, Mohamed Ali Jahromi, Mohammad Faseleh Ahmad, Yusof Ebrahimi, Mahdi Papaya (Carica papaya) leaf methanolic extract modulates in vitro rumen methanogenesis and rumen biohydrogenation |
| title | Papaya (Carica papaya) leaf methanolic extract modulates in vitro rumen methanogenesis and rumen biohydrogenation |
| title_full | Papaya (Carica papaya) leaf methanolic extract modulates in vitro rumen methanogenesis and rumen biohydrogenation |
| title_fullStr | Papaya (Carica papaya) leaf methanolic extract modulates in vitro rumen methanogenesis and rumen biohydrogenation |
| title_full_unstemmed | Papaya (Carica papaya) leaf methanolic extract modulates in vitro rumen methanogenesis and rumen biohydrogenation |
| title_short | Papaya (Carica papaya) leaf methanolic extract modulates in vitro rumen methanogenesis and rumen biohydrogenation |
| title_sort | papaya (carica papaya) leaf methanolic extract modulates in vitro rumen methanogenesis and rumen biohydrogenation |
| url | http://psasir.upm.edu.my/id/eprint/52023/ http://psasir.upm.edu.my/id/eprint/52023/ http://psasir.upm.edu.my/id/eprint/52023/ http://psasir.upm.edu.my/id/eprint/52023/1/Papaya%20%28Carica%20papaya%29%20leaf%20methanolic%20extract%20modulates%20in%20vitro%20rumen%20methanogenesis%20and%20rumen%20biohydrogenation.pdf |