Combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer MCF-7 cells in vitro

Despite progressive research being done on drug therapy to treat breast cancer, the number of patients succumbing to the disease is still a major issue. Combinatorial treatment using different drugs and herbs to treat cancer patients is of major interest in scientists nowadays. Doxorubicin is one of...

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Main Authors: Abdul Aziz, Muhammad Yusran, Abu, Nadiah, Yeap, Swee Keong, Ho, Wan Yong, Omar, Abdul Rahman, Ismail, Nor Hadiani, Ahmad, Syahida, Pirozyan, Mehdi Rasoli, Akhtar, Muhammad Nadeem, Mohammed Alitheen, Noorjahan Banu
Format: Article
Language:English
Published: MDPI 2016
Online Access:http://psasir.upm.edu.my/id/eprint/52003/
http://psasir.upm.edu.my/id/eprint/52003/1/52003.pdf
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author Abdul Aziz, Muhammad Yusran
Abu, Nadiah
Yeap, Swee Keong
Ho, Wan Yong
Omar, Abdul Rahman
Ismail, Nor Hadiani
Ahmad, Syahida
Pirozyan, Mehdi Rasoli
Akhtar, Muhammad Nadeem
Mohammed Alitheen, Noorjahan Banu
author_facet Abdul Aziz, Muhammad Yusran
Abu, Nadiah
Yeap, Swee Keong
Ho, Wan Yong
Omar, Abdul Rahman
Ismail, Nor Hadiani
Ahmad, Syahida
Pirozyan, Mehdi Rasoli
Akhtar, Muhammad Nadeem
Mohammed Alitheen, Noorjahan Banu
author_sort Abdul Aziz, Muhammad Yusran
building UPM Institutional Repository
collection Online Access
description Despite progressive research being done on drug therapy to treat breast cancer, the number of patients succumbing to the disease is still a major issue. Combinatorial treatment using different drugs and herbs to treat cancer patients is of major interest in scientists nowadays. Doxorubicin is one of the most used drugs to treat breast cancer patients. The combination of doxorubicin to other drugs such as tamoxifen has been reported. Nevertheless, the combination of doxorubicin with a natural product-derived agent has not been studied yet. Morinda citrifolia has always been sought out for its remarkable remedies. Damnacanthal, an anthraquinone that can be extracted from the roots of Morinda citrifolia is a promising compound that possesses a variety of biological properties. This study aimed to study the therapeutic effects of damnacanthal in combination with doxorubicin in breast cancer cells. Collectively, the combination of both these molecules enhanced the efficacy of induced cell death in MCF-7 as evidenced by the MTT assay, cell cycle, annexin V and expression of apoptosis-related genes and proteins. The effectiveness of doxorubicin as an anti-cancer drug was increased upon addition of damnacanthal. These results could provide a promising approach to treat breast cancer patients.
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spelling upm-520032017-05-04T08:40:53Z http://psasir.upm.edu.my/id/eprint/52003/ Combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer MCF-7 cells in vitro Abdul Aziz, Muhammad Yusran Abu, Nadiah Yeap, Swee Keong Ho, Wan Yong Omar, Abdul Rahman Ismail, Nor Hadiani Ahmad, Syahida Pirozyan, Mehdi Rasoli Akhtar, Muhammad Nadeem Mohammed Alitheen, Noorjahan Banu Despite progressive research being done on drug therapy to treat breast cancer, the number of patients succumbing to the disease is still a major issue. Combinatorial treatment using different drugs and herbs to treat cancer patients is of major interest in scientists nowadays. Doxorubicin is one of the most used drugs to treat breast cancer patients. The combination of doxorubicin to other drugs such as tamoxifen has been reported. Nevertheless, the combination of doxorubicin with a natural product-derived agent has not been studied yet. Morinda citrifolia has always been sought out for its remarkable remedies. Damnacanthal, an anthraquinone that can be extracted from the roots of Morinda citrifolia is a promising compound that possesses a variety of biological properties. This study aimed to study the therapeutic effects of damnacanthal in combination with doxorubicin in breast cancer cells. Collectively, the combination of both these molecules enhanced the efficacy of induced cell death in MCF-7 as evidenced by the MTT assay, cell cycle, annexin V and expression of apoptosis-related genes and proteins. The effectiveness of doxorubicin as an anti-cancer drug was increased upon addition of damnacanthal. These results could provide a promising approach to treat breast cancer patients. MDPI 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/52003/1/52003.pdf Abdul Aziz, Muhammad Yusran and Abu, Nadiah and Yeap, Swee Keong and Ho, Wan Yong and Omar, Abdul Rahman and Ismail, Nor Hadiani and Ahmad, Syahida and Pirozyan, Mehdi Rasoli and Akhtar, Muhammad Nadeem and Mohammed Alitheen, Noorjahan Banu (2016) Combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer MCF-7 cells in vitro. Molecules, 21 (9). art. no. 1228. pp. 1-15. ISSN 1420-3049 http://www.mdpi.com/1420-3049/21/9/1228 10.3390/molecules21091228
spellingShingle Abdul Aziz, Muhammad Yusran
Abu, Nadiah
Yeap, Swee Keong
Ho, Wan Yong
Omar, Abdul Rahman
Ismail, Nor Hadiani
Ahmad, Syahida
Pirozyan, Mehdi Rasoli
Akhtar, Muhammad Nadeem
Mohammed Alitheen, Noorjahan Banu
Combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer MCF-7 cells in vitro
title Combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer MCF-7 cells in vitro
title_full Combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer MCF-7 cells in vitro
title_fullStr Combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer MCF-7 cells in vitro
title_full_unstemmed Combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer MCF-7 cells in vitro
title_short Combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer MCF-7 cells in vitro
title_sort combinatorial cytotoxic effects of damnacanthal and doxorubicin against human breast cancer mcf-7 cells in vitro
url http://psasir.upm.edu.my/id/eprint/52003/
http://psasir.upm.edu.my/id/eprint/52003/
http://psasir.upm.edu.my/id/eprint/52003/
http://psasir.upm.edu.my/id/eprint/52003/1/52003.pdf