Physiological and immunoblot analyses of a nitric oxide synthase (NOS)-like protein of pea (Pisum sativum L.)
Nitric oxide (NO) functions as a signal molecule in different biological processes in plants, including disease resistance. Its production is related to nitric oxide synthase (NOS). The application of chemicals that induce systemic resistance in plants did not induce NOS activity in pea, suggesting...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Sains Malaysia
2006
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| Online Access: | http://psasir.upm.edu.my/id/eprint/51942/ http://psasir.upm.edu.my/id/eprint/51942/1/Physiological%20and%20immunoblot%20analyses%20of%20a%20nitric%20oxide%20synthase%20%28NOS%29-like%20protein%20of%20pea%20%28Pisum%20sativum%20L.%29.pdf |
| _version_ | 1848851970650013696 |
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| author | Wong, Mui Yun Huang, Jeng Sheng Davis, Eric L. |
| author_facet | Wong, Mui Yun Huang, Jeng Sheng Davis, Eric L. |
| author_sort | Wong, Mui Yun |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Nitric oxide (NO) functions as a signal molecule in different biological processes in plants, including disease resistance. Its production is related to nitric oxide synthase (NOS). The application of chemicals that induce systemic resistance in plants did not induce NOS activity in pea, suggesting that NO functions upstream of salicylic acid (SA) in the signaling pathway of defense responses in plants. NOS activity was induced in both the incompatible and compatible interactions of pea with Ralstonia solanacearum and Pseudomonas syringae pv pisi, respectively, between 3 h to 6 h post-infiltration, indicating that NOS was involved in both resistance and disease development responses in pea. Antibodies raised against mammalian NOS did not have specificity in detecting a NOS-like protein in pea, suggesting that the pea NOS-like protein could be structurally different from mammalian NOS, and immunodetection of a plant NOS-like protein must be conducted with caution and verified with functional assays. |
| first_indexed | 2025-11-15T10:30:39Z |
| format | Article |
| id | upm-51942 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:30:39Z |
| publishDate | 2006 |
| publisher | Penerbit Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-519422017-05-03T09:22:05Z http://psasir.upm.edu.my/id/eprint/51942/ Physiological and immunoblot analyses of a nitric oxide synthase (NOS)-like protein of pea (Pisum sativum L.) Wong, Mui Yun Huang, Jeng Sheng Davis, Eric L. Nitric oxide (NO) functions as a signal molecule in different biological processes in plants, including disease resistance. Its production is related to nitric oxide synthase (NOS). The application of chemicals that induce systemic resistance in plants did not induce NOS activity in pea, suggesting that NO functions upstream of salicylic acid (SA) in the signaling pathway of defense responses in plants. NOS activity was induced in both the incompatible and compatible interactions of pea with Ralstonia solanacearum and Pseudomonas syringae pv pisi, respectively, between 3 h to 6 h post-infiltration, indicating that NOS was involved in both resistance and disease development responses in pea. Antibodies raised against mammalian NOS did not have specificity in detecting a NOS-like protein in pea, suggesting that the pea NOS-like protein could be structurally different from mammalian NOS, and immunodetection of a plant NOS-like protein must be conducted with caution and verified with functional assays. Penerbit Universiti Sains Malaysia 2006 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/51942/1/Physiological%20and%20immunoblot%20analyses%20of%20a%20nitric%20oxide%20synthase%20%28NOS%29-like%20protein%20of%20pea%20%28Pisum%20sativum%20L.%29.pdf Wong, Mui Yun and Huang, Jeng Sheng and Davis, Eric L. (2006) Physiological and immunoblot analyses of a nitric oxide synthase (NOS)-like protein of pea (Pisum sativum L.). Journal of Bioscience, 17 (1). pp. 77-87. ISSN 1985-3718; ESSN: 2180-4249 http://www.tlsr.usm.my/TLSRvol17no1.html |
| spellingShingle | Wong, Mui Yun Huang, Jeng Sheng Davis, Eric L. Physiological and immunoblot analyses of a nitric oxide synthase (NOS)-like protein of pea (Pisum sativum L.) |
| title | Physiological and immunoblot analyses of a nitric oxide synthase (NOS)-like protein of pea (Pisum sativum L.) |
| title_full | Physiological and immunoblot analyses of a nitric oxide synthase (NOS)-like protein of pea (Pisum sativum L.) |
| title_fullStr | Physiological and immunoblot analyses of a nitric oxide synthase (NOS)-like protein of pea (Pisum sativum L.) |
| title_full_unstemmed | Physiological and immunoblot analyses of a nitric oxide synthase (NOS)-like protein of pea (Pisum sativum L.) |
| title_short | Physiological and immunoblot analyses of a nitric oxide synthase (NOS)-like protein of pea (Pisum sativum L.) |
| title_sort | physiological and immunoblot analyses of a nitric oxide synthase (nos)-like protein of pea (pisum sativum l.) |
| url | http://psasir.upm.edu.my/id/eprint/51942/ http://psasir.upm.edu.my/id/eprint/51942/ http://psasir.upm.edu.my/id/eprint/51942/1/Physiological%20and%20immunoblot%20analyses%20of%20a%20nitric%20oxide%20synthase%20%28NOS%29-like%20protein%20of%20pea%20%28Pisum%20sativum%20L.%29.pdf |