Kinetics of cellulase production by Chaetomium globosum at different levels of dissolved oxygen tension using oil palm empty fruit bunch fibre as substrate
The effect of different levels of dissolved oxygen tension (d.o.t) at a fixed agitation speed on the production of three major components of extracellular (free) cellulase (FPase, endoglucanase and β-glucosidase) by Chaetomium globosum strain 414 was investigated. Oil palm empty fruit bunch (OPEFB)...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Rapid Science Publishers
1998
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/51777/ http://psasir.upm.edu.my/id/eprint/51777/1/51777.pdf http://psasir.upm.edu.my/id/eprint/51777/7/A_1008871528360.pdf |
| _version_ | 1848851923294224384 |
|---|---|
| author | M. S., Umikalsom Ariff, A. B. Hassan, M. A. A. Karim, M. I. |
| author_facet | M. S., Umikalsom Ariff, A. B. Hassan, M. A. A. Karim, M. I. |
| author_sort | M. S., Umikalsom |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The effect of different levels of dissolved oxygen tension (d.o.t) at a fixed agitation speed on the production of three major components of extracellular (free) cellulase (FPase, endoglucanase and β-glucosidase) by Chaetomium globosum strain 414 was investigated. Oil palm empty fruit bunch (OPEFB) fibre (10 g/l) and peptone (6 g/l) were used as carbon and nitrogen sources for cellulase production, respectively. Growth of C. globosum and cellulase complex production were modelled using models based on logistic and Luedeking-Piret equations. The calculated data fitted well to the experimental data, indicating that the models were suitable for describing and verifying growth and cellulase complex production at different d.o.t levels. Cell growth and cellulase production were approximately two-fold higher in the stirred tank fermenter as compared with shake flask culture. At d.o.t of between 30-50% saturation, cell growth and cellulase production were higher than those under oxygen-limited conditions (5% saturation) and at high d.o.t (80% saturation). However, the highest activities of FPase (2.5 U/ml), CMCase (59.5 U/ml) and β-glucosidase (12.8 U/ml) were obtained at a d.o.t of 50% saturation and these gave the overall productivities of 20.8, 495 and 53.3 U/l.h, respectively. |
| first_indexed | 2025-11-15T10:29:54Z |
| format | Article |
| id | upm-51777 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T10:29:54Z |
| publishDate | 1998 |
| publisher | Rapid Science Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-517772024-08-07T06:29:09Z http://psasir.upm.edu.my/id/eprint/51777/ Kinetics of cellulase production by Chaetomium globosum at different levels of dissolved oxygen tension using oil palm empty fruit bunch fibre as substrate M. S., Umikalsom Ariff, A. B. Hassan, M. A. A. Karim, M. I. The effect of different levels of dissolved oxygen tension (d.o.t) at a fixed agitation speed on the production of three major components of extracellular (free) cellulase (FPase, endoglucanase and β-glucosidase) by Chaetomium globosum strain 414 was investigated. Oil palm empty fruit bunch (OPEFB) fibre (10 g/l) and peptone (6 g/l) were used as carbon and nitrogen sources for cellulase production, respectively. Growth of C. globosum and cellulase complex production were modelled using models based on logistic and Luedeking-Piret equations. The calculated data fitted well to the experimental data, indicating that the models were suitable for describing and verifying growth and cellulase complex production at different d.o.t levels. Cell growth and cellulase production were approximately two-fold higher in the stirred tank fermenter as compared with shake flask culture. At d.o.t of between 30-50% saturation, cell growth and cellulase production were higher than those under oxygen-limited conditions (5% saturation) and at high d.o.t (80% saturation). However, the highest activities of FPase (2.5 U/ml), CMCase (59.5 U/ml) and β-glucosidase (12.8 U/ml) were obtained at a d.o.t of 50% saturation and these gave the overall productivities of 20.8, 495 and 53.3 U/l.h, respectively. Rapid Science Publishers 1998 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/51777/1/51777.pdf text en http://psasir.upm.edu.my/id/eprint/51777/7/A_1008871528360.pdf M. S., Umikalsom and Ariff, A. B. and Hassan, M. A. and A. Karim, M. I. (1998) Kinetics of cellulase production by Chaetomium globosum at different levels of dissolved oxygen tension using oil palm empty fruit bunch fibre as substrate. World Journal of Microbiology and Biotechnology, 14 (4). pp. 491-498. ISSN 0959-3993; ESSN: 1573-0972 https://rd.springer.com/article/10.1023/A:1008871528360 10.1023/A:1008871528360 |
| spellingShingle | M. S., Umikalsom Ariff, A. B. Hassan, M. A. A. Karim, M. I. Kinetics of cellulase production by Chaetomium globosum at different levels of dissolved oxygen tension using oil palm empty fruit bunch fibre as substrate |
| title | Kinetics of cellulase production by Chaetomium globosum at different levels of dissolved oxygen tension using oil palm empty fruit bunch fibre as substrate |
| title_full | Kinetics of cellulase production by Chaetomium globosum at different levels of dissolved oxygen tension using oil palm empty fruit bunch fibre as substrate |
| title_fullStr | Kinetics of cellulase production by Chaetomium globosum at different levels of dissolved oxygen tension using oil palm empty fruit bunch fibre as substrate |
| title_full_unstemmed | Kinetics of cellulase production by Chaetomium globosum at different levels of dissolved oxygen tension using oil palm empty fruit bunch fibre as substrate |
| title_short | Kinetics of cellulase production by Chaetomium globosum at different levels of dissolved oxygen tension using oil palm empty fruit bunch fibre as substrate |
| title_sort | kinetics of cellulase production by chaetomium globosum at different levels of dissolved oxygen tension using oil palm empty fruit bunch fibre as substrate |
| url | http://psasir.upm.edu.my/id/eprint/51777/ http://psasir.upm.edu.my/id/eprint/51777/ http://psasir.upm.edu.my/id/eprint/51777/ http://psasir.upm.edu.my/id/eprint/51777/1/51777.pdf http://psasir.upm.edu.my/id/eprint/51777/7/A_1008871528360.pdf |