Molecular analysis of Dichelobacter nodosus isolated from footrot in sheep in Malaysia
Pulsed field gel electrophoresis analysis of genomic DNA was used to investigate genetic diversity among Dichelobacter nodosus from footrot in sheep in Malaysia. Twelve Dichelobacter nodosus strains isolated from lesion materials from infected sheep were confirmed as Dichelobacter nodosus by polymer...
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| Format: | Article |
| Language: | English English English |
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Elsevier Science
1998
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| Online Access: | http://psasir.upm.edu.my/id/eprint/51688/ http://psasir.upm.edu.my/id/eprint/51688/1/51688.pdf http://psasir.upm.edu.my/id/eprint/51688/7/1-s2.0-S0378113598002193-main.pdf http://psasir.upm.edu.my/id/eprint/51688/8/1-s2.0-S0378113598002193-main.pdf |
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| author | Zakaria, Zunita Radu, Son Abdul Rahman, Sheikh Omar Mutalib, Abdul Rahim Joseph, P. G. Rahmat Ali, Gulam Rusul |
| author_facet | Zakaria, Zunita Radu, Son Abdul Rahman, Sheikh Omar Mutalib, Abdul Rahim Joseph, P. G. Rahmat Ali, Gulam Rusul |
| author_sort | Zakaria, Zunita |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pulsed field gel electrophoresis analysis of genomic DNA was used to investigate genetic diversity among Dichelobacter nodosus from footrot in sheep in Malaysia. Twelve Dichelobacter nodosus strains isolated from lesion materials from infected sheep were confirmed as Dichelobacter nodosus by polymerase chain reaction technique using the species-specific Dichelobacter nodosus 16S RNA sequence Ac and C as primers. Pulsed field gel electrophoresis banding profiles using restriction enzymes ApaI (5′GGGCCC3′), SfiI (5′GGCCNNNNNGGCC3′)and SmaI (′5CCCGGG3′) enabled the 12 Dichelobacter nodosus strains to be differentiated into eight different PFGE patterns and thus genome-types, with F (coefficient of similarity) values ranging from 0.17 to 1.0 (ApaI), 0.14 to 1.0 (SfiI) and 0.22 to 1.0 (SmaI). Strains with origin in different farms were shown to have different PFGE patterns (two strains, M7 and M8 were the only exception). On the basis of their PFGE, all field strains used in the study differed from the reference strains. Our data revealed that there are several clonal types of Dichelobacter nodosus isolates and indicated that there is probably more than one source of this pathogen on the farms studied. The study showed that strains of D. nodosus exhibited considerable genetic diversity using this method and that genomic analysis by pulsed field gel electrophoresis was useful in discriminating the D. nodosus strains. |
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| format | Article |
| id | upm-51688 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-15T10:29:31Z |
| publishDate | 1998 |
| publisher | Elsevier Science |
| recordtype | eprints |
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| spelling | upm-516882024-08-07T08:27:14Z http://psasir.upm.edu.my/id/eprint/51688/ Molecular analysis of Dichelobacter nodosus isolated from footrot in sheep in Malaysia Zakaria, Zunita Radu, Son Abdul Rahman, Sheikh Omar Mutalib, Abdul Rahim Joseph, P. G. Rahmat Ali, Gulam Rusul Pulsed field gel electrophoresis analysis of genomic DNA was used to investigate genetic diversity among Dichelobacter nodosus from footrot in sheep in Malaysia. Twelve Dichelobacter nodosus strains isolated from lesion materials from infected sheep were confirmed as Dichelobacter nodosus by polymerase chain reaction technique using the species-specific Dichelobacter nodosus 16S RNA sequence Ac and C as primers. Pulsed field gel electrophoresis banding profiles using restriction enzymes ApaI (5′GGGCCC3′), SfiI (5′GGCCNNNNNGGCC3′)and SmaI (′5CCCGGG3′) enabled the 12 Dichelobacter nodosus strains to be differentiated into eight different PFGE patterns and thus genome-types, with F (coefficient of similarity) values ranging from 0.17 to 1.0 (ApaI), 0.14 to 1.0 (SfiI) and 0.22 to 1.0 (SmaI). Strains with origin in different farms were shown to have different PFGE patterns (two strains, M7 and M8 were the only exception). On the basis of their PFGE, all field strains used in the study differed from the reference strains. Our data revealed that there are several clonal types of Dichelobacter nodosus isolates and indicated that there is probably more than one source of this pathogen on the farms studied. The study showed that strains of D. nodosus exhibited considerable genetic diversity using this method and that genomic analysis by pulsed field gel electrophoresis was useful in discriminating the D. nodosus strains. Elsevier Science 1998 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/51688/1/51688.pdf text en http://psasir.upm.edu.my/id/eprint/51688/7/1-s2.0-S0378113598002193-main.pdf text en http://psasir.upm.edu.my/id/eprint/51688/8/1-s2.0-S0378113598002193-main.pdf Zakaria, Zunita and Radu, Son and Abdul Rahman, Sheikh Omar and Mutalib, Abdul Rahim and Joseph, P. G. and Rahmat Ali, Gulam Rusul (1998) Molecular analysis of Dichelobacter nodosus isolated from footrot in sheep in Malaysia. Veterinary Microbiology, 62 (3). pp. 243-250. ISSN 0378-1135; ESSN: 1873-2542 http://www.sciencedirect.com/science/article/pii/S0378113598002193?via%3Dihub 10.1016/S0378-1135(98)00219-3 |
| spellingShingle | Zakaria, Zunita Radu, Son Abdul Rahman, Sheikh Omar Mutalib, Abdul Rahim Joseph, P. G. Rahmat Ali, Gulam Rusul Molecular analysis of Dichelobacter nodosus isolated from footrot in sheep in Malaysia |
| title | Molecular analysis of Dichelobacter nodosus isolated from footrot in sheep in Malaysia |
| title_full | Molecular analysis of Dichelobacter nodosus isolated from footrot in sheep in Malaysia |
| title_fullStr | Molecular analysis of Dichelobacter nodosus isolated from footrot in sheep in Malaysia |
| title_full_unstemmed | Molecular analysis of Dichelobacter nodosus isolated from footrot in sheep in Malaysia |
| title_short | Molecular analysis of Dichelobacter nodosus isolated from footrot in sheep in Malaysia |
| title_sort | molecular analysis of dichelobacter nodosus isolated from footrot in sheep in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/51688/ http://psasir.upm.edu.my/id/eprint/51688/ http://psasir.upm.edu.my/id/eprint/51688/ http://psasir.upm.edu.my/id/eprint/51688/1/51688.pdf http://psasir.upm.edu.my/id/eprint/51688/7/1-s2.0-S0378113598002193-main.pdf http://psasir.upm.edu.my/id/eprint/51688/8/1-s2.0-S0378113598002193-main.pdf |