Characterization of Vibrio vulnificus isolated from cockles (Anadara granosa): antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis
Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA (RAPD) were used to study strains of Vibrio vulnificus isolated from cockles (Anadara granosa). Thirty-six isolates were analyzed. The prevalent biotypes were 1 (72.2% of the isolates) and 2 (27.8%). Among these,...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English English |
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Elsevier
1998
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| Online Access: | http://psasir.upm.edu.my/id/eprint/51676/ http://psasir.upm.edu.my/id/eprint/51676/1/51676.pdf http://psasir.upm.edu.my/id/eprint/51676/7/165-1-139.pdf |
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| author | Radu, Son Mohamed, Nasreldin Elhadi Hussein Hassan, Zaiton Rahmat Ali, Gulam Rusul Lihan, Samuel Fifadara, Nimita H. Yuherman Purwati, Endang |
| author_facet | Radu, Son Mohamed, Nasreldin Elhadi Hussein Hassan, Zaiton Rahmat Ali, Gulam Rusul Lihan, Samuel Fifadara, Nimita H. Yuherman Purwati, Endang |
| author_sort | Radu, Son |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA (RAPD) were used to study strains of Vibrio vulnificus isolated from cockles (Anadara granosa). Thirty-six isolates were analyzed. The prevalent biotypes were 1 (72.2% of the isolates) and 2 (27.8%). Among these, 21 strains of biotype 1 and two strains of biotype 2 contained plasmid DNA bands ranging in size from 1.4 to 9.7 MDa. Thirty-one (83.3%) were found to be resistant to one or more of the antimicrobial agents tested, however no specific correlation between antimicrobial resistance patterns and a single biotype was found. In addition, no particular plasmid profile was predictive of a particular pattern of antibiotic susceptibility. Two primers produced polymorphisms in all strains tested, producing bands ranging from 0.25 to 2.7 kb, indicating a high variability among both biotype 1 and biotype 2 of the V. vulnificus strains investigated. RAPD identity across biotypes was also observed among Vibrio vulnificus strains. |
| first_indexed | 2025-11-15T10:29:28Z |
| format | Article |
| id | upm-51676 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T10:29:28Z |
| publishDate | 1998 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-516762024-07-26T09:28:10Z http://psasir.upm.edu.my/id/eprint/51676/ Characterization of Vibrio vulnificus isolated from cockles (Anadara granosa): antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis Radu, Son Mohamed, Nasreldin Elhadi Hussein Hassan, Zaiton Rahmat Ali, Gulam Rusul Lihan, Samuel Fifadara, Nimita H. Yuherman Purwati, Endang Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA (RAPD) were used to study strains of Vibrio vulnificus isolated from cockles (Anadara granosa). Thirty-six isolates were analyzed. The prevalent biotypes were 1 (72.2% of the isolates) and 2 (27.8%). Among these, 21 strains of biotype 1 and two strains of biotype 2 contained plasmid DNA bands ranging in size from 1.4 to 9.7 MDa. Thirty-one (83.3%) were found to be resistant to one or more of the antimicrobial agents tested, however no specific correlation between antimicrobial resistance patterns and a single biotype was found. In addition, no particular plasmid profile was predictive of a particular pattern of antibiotic susceptibility. Two primers produced polymorphisms in all strains tested, producing bands ranging from 0.25 to 2.7 kb, indicating a high variability among both biotype 1 and biotype 2 of the V. vulnificus strains investigated. RAPD identity across biotypes was also observed among Vibrio vulnificus strains. Elsevier 1998 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/51676/1/51676.pdf text en http://psasir.upm.edu.my/id/eprint/51676/7/165-1-139.pdf Radu, Son and Mohamed, Nasreldin Elhadi Hussein and Hassan, Zaiton and Rahmat Ali, Gulam Rusul and Lihan, Samuel and Fifadara, Nimita H. and Yuherman and Purwati, Endang (1998) Characterization of Vibrio vulnificus isolated from cockles (Anadara granosa): antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis. FEMS Microbiology Letters, 165 (1). pp. 139-143. ISSN 0378-1097 http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1998.tb13138.x/abstract 10.1111/j.1574-6968.1998.tb13138.x |
| spellingShingle | Radu, Son Mohamed, Nasreldin Elhadi Hussein Hassan, Zaiton Rahmat Ali, Gulam Rusul Lihan, Samuel Fifadara, Nimita H. Yuherman Purwati, Endang Characterization of Vibrio vulnificus isolated from cockles (Anadara granosa): antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis |
| title | Characterization of Vibrio vulnificus isolated from cockles (Anadara granosa): antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis |
| title_full | Characterization of Vibrio vulnificus isolated from cockles (Anadara granosa): antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis |
| title_fullStr | Characterization of Vibrio vulnificus isolated from cockles (Anadara granosa): antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis |
| title_full_unstemmed | Characterization of Vibrio vulnificus isolated from cockles (Anadara granosa): antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis |
| title_short | Characterization of Vibrio vulnificus isolated from cockles (Anadara granosa): antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis |
| title_sort | characterization of vibrio vulnificus isolated from cockles (anadara granosa): antimicrobial resistance, plasmid profiles and random amplification of polymorphic dna analysis |
| url | http://psasir.upm.edu.my/id/eprint/51676/ http://psasir.upm.edu.my/id/eprint/51676/ http://psasir.upm.edu.my/id/eprint/51676/ http://psasir.upm.edu.my/id/eprint/51676/1/51676.pdf http://psasir.upm.edu.my/id/eprint/51676/7/165-1-139.pdf |