Effect of oligosaccharides on glucose consumption by Rhodobacter sphaeroides in polyhydroxyalkanoate production from enzymatically treated crude sago starch
A method of producing a bacterial polyester, polyhydroxyalkanoate (PHA), from enzymatically treated crude sago starch using the photosynthetic bacterium Rhodobacter sphaeroides (IFO 12203) is described. When α-amylase and glucoamylase were used in sequence to saccharify sago starch, the isomaltose t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
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Society for Fermentation and Bioengineering
1998
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| Online Access: | http://psasir.upm.edu.my/id/eprint/51660/ http://psasir.upm.edu.my/id/eprint/51660/1/51660.pdf http://psasir.upm.edu.my/id/eprint/51660/7/1-s2.0-S0922338X98800342-main.pdf |
| _version_ | 1848851891566411776 |
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| author | Hassan, Mohd Ali Shirai, Yoshihito Kubota, Asaka Abdul Karim, Mohamed Ismail Nakanishi, Kazuhiro Hashimoto, Kenji |
| author_facet | Hassan, Mohd Ali Shirai, Yoshihito Kubota, Asaka Abdul Karim, Mohamed Ismail Nakanishi, Kazuhiro Hashimoto, Kenji |
| author_sort | Hassan, Mohd Ali |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A method of producing a bacterial polyester, polyhydroxyalkanoate (PHA), from enzymatically treated crude sago starch using the photosynthetic bacterium Rhodobacter sphaeroides (IFO 12203) is described. When α-amylase and glucoamylase were used in sequence to saccharify sago starch, the isomaltose thus produced was found to enhance glucose consumption by the cells, resulting in increased volumetric PHA productivity. On the other hand, treatment with a mixture of glucoamylase and pullulanase during the saccharification of sago starch resulted in the production of glucose but no isomaltose. Using this solution, glucose consumption by R. sphaeroides was lower than that using the solution treated with α-amylase + glucoamylase. These results suggested that isomaltose (with 1-6 bonding) contributed to the enhancement of the glucose consumption rate and PHA yield. This was experimentally confirmed by cultivating R. sphaeroides in a medium containing glucose with either isomaltose or malto-oligosaccharides without 1-6 bonding. |
| first_indexed | 2025-11-15T10:29:23Z |
| format | Article |
| id | upm-51660 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T10:29:23Z |
| publishDate | 1998 |
| publisher | Society for Fermentation and Bioengineering |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-516602024-08-07T00:13:44Z http://psasir.upm.edu.my/id/eprint/51660/ Effect of oligosaccharides on glucose consumption by Rhodobacter sphaeroides in polyhydroxyalkanoate production from enzymatically treated crude sago starch Hassan, Mohd Ali Shirai, Yoshihito Kubota, Asaka Abdul Karim, Mohamed Ismail Nakanishi, Kazuhiro Hashimoto, Kenji A method of producing a bacterial polyester, polyhydroxyalkanoate (PHA), from enzymatically treated crude sago starch using the photosynthetic bacterium Rhodobacter sphaeroides (IFO 12203) is described. When α-amylase and glucoamylase were used in sequence to saccharify sago starch, the isomaltose thus produced was found to enhance glucose consumption by the cells, resulting in increased volumetric PHA productivity. On the other hand, treatment with a mixture of glucoamylase and pullulanase during the saccharification of sago starch resulted in the production of glucose but no isomaltose. Using this solution, glucose consumption by R. sphaeroides was lower than that using the solution treated with α-amylase + glucoamylase. These results suggested that isomaltose (with 1-6 bonding) contributed to the enhancement of the glucose consumption rate and PHA yield. This was experimentally confirmed by cultivating R. sphaeroides in a medium containing glucose with either isomaltose or malto-oligosaccharides without 1-6 bonding. Society for Fermentation and Bioengineering 1998 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/51660/1/51660.pdf text en http://psasir.upm.edu.my/id/eprint/51660/7/1-s2.0-S0922338X98800342-main.pdf Hassan, Mohd Ali and Shirai, Yoshihito and Kubota, Asaka and Abdul Karim, Mohamed Ismail and Nakanishi, Kazuhiro and Hashimoto, Kenji (1998) Effect of oligosaccharides on glucose consumption by Rhodobacter sphaeroides in polyhydroxyalkanoate production from enzymatically treated crude sago starch. Journal of Fermentation and Bioengineering, 86 (1). pp. 57-61. ISSN 0922-338X http://www.sciencedirect.com/science/article/pii/S0922338X98800342 10.1016/S0922-338X(98)80034-2 |
| spellingShingle | Hassan, Mohd Ali Shirai, Yoshihito Kubota, Asaka Abdul Karim, Mohamed Ismail Nakanishi, Kazuhiro Hashimoto, Kenji Effect of oligosaccharides on glucose consumption by Rhodobacter sphaeroides in polyhydroxyalkanoate production from enzymatically treated crude sago starch |
| title | Effect of oligosaccharides on glucose consumption by Rhodobacter sphaeroides in polyhydroxyalkanoate production from enzymatically treated crude sago starch |
| title_full | Effect of oligosaccharides on glucose consumption by Rhodobacter sphaeroides in polyhydroxyalkanoate production from enzymatically treated crude sago starch |
| title_fullStr | Effect of oligosaccharides on glucose consumption by Rhodobacter sphaeroides in polyhydroxyalkanoate production from enzymatically treated crude sago starch |
| title_full_unstemmed | Effect of oligosaccharides on glucose consumption by Rhodobacter sphaeroides in polyhydroxyalkanoate production from enzymatically treated crude sago starch |
| title_short | Effect of oligosaccharides on glucose consumption by Rhodobacter sphaeroides in polyhydroxyalkanoate production from enzymatically treated crude sago starch |
| title_sort | effect of oligosaccharides on glucose consumption by rhodobacter sphaeroides in polyhydroxyalkanoate production from enzymatically treated crude sago starch |
| url | http://psasir.upm.edu.my/id/eprint/51660/ http://psasir.upm.edu.my/id/eprint/51660/ http://psasir.upm.edu.my/id/eprint/51660/ http://psasir.upm.edu.my/id/eprint/51660/1/51660.pdf http://psasir.upm.edu.my/id/eprint/51660/7/1-s2.0-S0922338X98800342-main.pdf |