Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into th...
| Main Authors: | , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/51310/ http://psasir.upm.edu.my/id/eprint/51310/1/P011%20pg71-76.pdf |
| _version_ | 1848851797942206464 |
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| author | Wan Omar, Wan Siti Atikah Sulaiman, Ahmad Ziad Ajit @ Abd Aziz, Azilah Chisti, Yusuf Ab Rahim, Mohd Hairul Leow, Adam Thean Chor |
| author_facet | Wan Omar, Wan Siti Atikah Sulaiman, Ahmad Ziad Ajit @ Abd Aziz, Azilah Chisti, Yusuf Ab Rahim, Mohd Hairul Leow, Adam Thean Chor |
| author_sort | Wan Omar, Wan Siti Atikah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into the same strain of competent cells would assist in research assessment. This study focused on the transformation of recombinant lipase from two different sources into Escherichia coli competent cells, BL21 (DE3). The recombinant cells, pUC8.2-14 (American Type Culture Collection (ATCC)®68046™) was harbouring Rhizopus (delemar) oryzae lipase and E. coli Top 10 pTrcHis was harbouring Staphylococcus hyicus lipase, respectively. The transformation is involved preparation of competent cells, polymerase chain reaction (PCR) and DNA sequencing. The DNA sequences obtained was inserted into Basic Local Alignment Search Tool (BLAST), under the databases of National Centre for Biotechnology Information (NCBI), USA. The findings show the respective plasmids were successfully carried by the E. coli BL21 (DE3) for further investigation. |
| first_indexed | 2025-11-15T10:27:54Z |
| format | Conference or Workshop Item |
| id | upm-51310 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:27:54Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-513102017-04-03T07:48:05Z http://psasir.upm.edu.my/id/eprint/51310/ Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) Wan Omar, Wan Siti Atikah Sulaiman, Ahmad Ziad Ajit @ Abd Aziz, Azilah Chisti, Yusuf Ab Rahim, Mohd Hairul Leow, Adam Thean Chor Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into the same strain of competent cells would assist in research assessment. This study focused on the transformation of recombinant lipase from two different sources into Escherichia coli competent cells, BL21 (DE3). The recombinant cells, pUC8.2-14 (American Type Culture Collection (ATCC)®68046™) was harbouring Rhizopus (delemar) oryzae lipase and E. coli Top 10 pTrcHis was harbouring Staphylococcus hyicus lipase, respectively. The transformation is involved preparation of competent cells, polymerase chain reaction (PCR) and DNA sequencing. The DNA sequences obtained was inserted into Basic Local Alignment Search Tool (BLAST), under the databases of National Centre for Biotechnology Information (NCBI), USA. The findings show the respective plasmids were successfully carried by the E. coli BL21 (DE3) for further investigation. 2016 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/51310/1/P011%20pg71-76.pdf Wan Omar, Wan Siti Atikah and Sulaiman, Ahmad Ziad and Ajit @ Abd Aziz, Azilah and Chisti, Yusuf and Ab Rahim, Mohd Hairul and Leow, Adam Thean Chor (2016) Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3). In: 3rd National Conference for Postgraduate Research (NCON-PGR2016), 24-25 Sept. 2016, Universiti Malaysia Pahang, Pekan, Pahang. (pp. 71-76). http://ee.ump.edu.my/ncon/download/ |
| spellingShingle | Wan Omar, Wan Siti Atikah Sulaiman, Ahmad Ziad Ajit @ Abd Aziz, Azilah Chisti, Yusuf Ab Rahim, Mohd Hairul Leow, Adam Thean Chor Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) |
| title | Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) |
| title_full | Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) |
| title_fullStr | Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) |
| title_full_unstemmed | Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) |
| title_short | Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) |
| title_sort | transformation of ptrchis and puc8.2-14 into escherichia coli bl21 (de3) |
| url | http://psasir.upm.edu.my/id/eprint/51310/ http://psasir.upm.edu.my/id/eprint/51310/ http://psasir.upm.edu.my/id/eprint/51310/1/P011%20pg71-76.pdf |