Enzymatic synthesis of fatty esters by alkylated lipase
Lipase from Candida rugosa was chemically modified by reductive alkylation with aldehydes of various chain lengths. The derivatised lipases showed a higher esterification activity compared to native enzyme. The degree of activity enhancement depended on the type and molecular weight of the modifiers...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Elsevier Science
1997
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| Online Access: | http://psasir.upm.edu.my/id/eprint/51109/ http://psasir.upm.edu.my/id/eprint/51109/1/51109.pdf http://psasir.upm.edu.my/id/eprint/51109/7/1-s2.0-S1381117796000549-main.pdf |
| _version_ | 1848851744319078400 |
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| author | Basri, Mahiran Ampon, Kamaruzaman Wan Yunus, Wan Md. Zin Abdul Razak, Che Nyonya Salleh, Abu Bakar |
| author_facet | Basri, Mahiran Ampon, Kamaruzaman Wan Yunus, Wan Md. Zin Abdul Razak, Che Nyonya Salleh, Abu Bakar |
| author_sort | Basri, Mahiran |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Lipase from Candida rugosa was chemically modified by reductive alkylation with aldehydes of various chain lengths. The derivatised lipases showed a higher esterification activity compared to native enzyme. The degree of activity enhancement depended on the type and molecular weight of the modifiers used and the degree of modification of the enzyme. They exhibited higher activities in non-polar than in polar solvents. The optimum esterification temperature and preference of fatty acids as acyl donors of the derivatised lipases were very similar to those of native enzyme. Lipase derivatised with dodecyldehyde was more thermostable than those modified with acetaldehyde. Alkylated lipases are relatively more stable in organic solvents than the native enzyme. |
| first_indexed | 2025-11-15T10:27:03Z |
| format | Article |
| id | upm-51109 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T10:27:03Z |
| publishDate | 1997 |
| publisher | Elsevier Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-511092024-08-07T07:35:34Z http://psasir.upm.edu.my/id/eprint/51109/ Enzymatic synthesis of fatty esters by alkylated lipase Basri, Mahiran Ampon, Kamaruzaman Wan Yunus, Wan Md. Zin Abdul Razak, Che Nyonya Salleh, Abu Bakar Lipase from Candida rugosa was chemically modified by reductive alkylation with aldehydes of various chain lengths. The derivatised lipases showed a higher esterification activity compared to native enzyme. The degree of activity enhancement depended on the type and molecular weight of the modifiers used and the degree of modification of the enzyme. They exhibited higher activities in non-polar than in polar solvents. The optimum esterification temperature and preference of fatty acids as acyl donors of the derivatised lipases were very similar to those of native enzyme. Lipase derivatised with dodecyldehyde was more thermostable than those modified with acetaldehyde. Alkylated lipases are relatively more stable in organic solvents than the native enzyme. Elsevier Science 1997 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/51109/1/51109.pdf text en http://psasir.upm.edu.my/id/eprint/51109/7/1-s2.0-S1381117796000549-main.pdf Basri, Mahiran and Ampon, Kamaruzaman and Wan Yunus, Wan Md. Zin and Abdul Razak, Che Nyonya and Salleh, Abu Bakar (1997) Enzymatic synthesis of fatty esters by alkylated lipase. Journal of Molecular Catalysis B: Enzymatic, 3 (1-4). pp. 171-176. ISSN 1381-1177 http://www.sciencedirect.com/science/article/pii/S1381117796000549?via%3Dihub 10.1016/S1381-1177(96)00054-9 |
| spellingShingle | Basri, Mahiran Ampon, Kamaruzaman Wan Yunus, Wan Md. Zin Abdul Razak, Che Nyonya Salleh, Abu Bakar Enzymatic synthesis of fatty esters by alkylated lipase |
| title | Enzymatic synthesis of fatty esters by alkylated lipase |
| title_full | Enzymatic synthesis of fatty esters by alkylated lipase |
| title_fullStr | Enzymatic synthesis of fatty esters by alkylated lipase |
| title_full_unstemmed | Enzymatic synthesis of fatty esters by alkylated lipase |
| title_short | Enzymatic synthesis of fatty esters by alkylated lipase |
| title_sort | enzymatic synthesis of fatty esters by alkylated lipase |
| url | http://psasir.upm.edu.my/id/eprint/51109/ http://psasir.upm.edu.my/id/eprint/51109/ http://psasir.upm.edu.my/id/eprint/51109/ http://psasir.upm.edu.my/id/eprint/51109/1/51109.pdf http://psasir.upm.edu.my/id/eprint/51109/7/1-s2.0-S1381117796000549-main.pdf |