Purification and properties of pectinesterase from soursop (Anona muricata) pulp
Two forms of pectinesterase were purified using the techniques of ammonium sulphate fractionation, ion-exchange chromatography and gel filtration. PE I had a specific activity of approximately 4 units mg−1 (43-fold), that of PE II was 6.4 units mg−1 (229-fold). These pectinesterases (PE I and PE II)...
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| Format: | Article |
| Language: | English English |
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Elsevier Science
1997
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| Online Access: | http://psasir.upm.edu.my/id/eprint/51107/ http://psasir.upm.edu.my/id/eprint/51107/1/0000.pdf http://psasir.upm.edu.my/id/eprint/51107/7/1-s2.0-S030881469600043X-main.pdf |
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| author | Misman, Siti Arbaisah Ali, Asbi Abdul Hamid, Junainah Bakar, Jamilah |
| author_facet | Misman, Siti Arbaisah Ali, Asbi Abdul Hamid, Junainah Bakar, Jamilah |
| author_sort | Misman, Siti Arbaisah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Two forms of pectinesterase were purified using the techniques of ammonium sulphate fractionation, ion-exchange chromatography and gel filtration. PE I had a specific activity of approximately 4 units mg−1 (43-fold), that of PE II was 6.4 units mg−1 (229-fold). These pectinesterases (PE I and PE II) had approximate molecular weights of 29 100 and 24 100, respectively, as estimated by gel filtration, and 31 000 and 28 000, respectively, as estimated by sodium dodecyl sulphate polyacrylamide electrophoresis. The optimum temperature for enzyme activity was shown to be 60 °C for both PE I and PE II. The activation energies of PE I and PE II were calculated as 36 kJ mol−1 and 42 kJ mol−1, respectively. The optimum pH values for both pectinesterases lie within the range 7.0–8.0. The Km value for PE I was 0.52 mg ml−1 and 0.0843 mg ml−1 for PE II. PE I had a maximum velocity (Vmax) of 154 μmol mg−1 min−1, and PE II a Vmax of 726 μmol mg−1 min−1. |
| first_indexed | 2025-11-15T10:27:02Z |
| format | Article |
| id | upm-51107 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T10:27:02Z |
| publishDate | 1997 |
| publisher | Elsevier Science |
| recordtype | eprints |
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| spelling | upm-511072024-08-06T23:55:46Z http://psasir.upm.edu.my/id/eprint/51107/ Purification and properties of pectinesterase from soursop (Anona muricata) pulp Misman, Siti Arbaisah Ali, Asbi Abdul Hamid, Junainah Bakar, Jamilah Two forms of pectinesterase were purified using the techniques of ammonium sulphate fractionation, ion-exchange chromatography and gel filtration. PE I had a specific activity of approximately 4 units mg−1 (43-fold), that of PE II was 6.4 units mg−1 (229-fold). These pectinesterases (PE I and PE II) had approximate molecular weights of 29 100 and 24 100, respectively, as estimated by gel filtration, and 31 000 and 28 000, respectively, as estimated by sodium dodecyl sulphate polyacrylamide electrophoresis. The optimum temperature for enzyme activity was shown to be 60 °C for both PE I and PE II. The activation energies of PE I and PE II were calculated as 36 kJ mol−1 and 42 kJ mol−1, respectively. The optimum pH values for both pectinesterases lie within the range 7.0–8.0. The Km value for PE I was 0.52 mg ml−1 and 0.0843 mg ml−1 for PE II. PE I had a maximum velocity (Vmax) of 154 μmol mg−1 min−1, and PE II a Vmax of 726 μmol mg−1 min−1. Elsevier Science 1997 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/51107/1/0000.pdf text en http://psasir.upm.edu.my/id/eprint/51107/7/1-s2.0-S030881469600043X-main.pdf Misman, Siti Arbaisah and Ali, Asbi and Abdul Hamid, Junainah and Bakar, Jamilah (1997) Purification and properties of pectinesterase from soursop (Anona muricata) pulp. Food Chemistry, 59 (1). pp. 33-40. ISSN 0308-8146 http://www.sciencedirect.com/science/article/pii/S030881469600043X 10.1016/S0308-8146(96)00043-X |
| spellingShingle | Misman, Siti Arbaisah Ali, Asbi Abdul Hamid, Junainah Bakar, Jamilah Purification and properties of pectinesterase from soursop (Anona muricata) pulp |
| title | Purification and properties of pectinesterase from soursop (Anona muricata) pulp |
| title_full | Purification and properties of pectinesterase from soursop (Anona muricata) pulp |
| title_fullStr | Purification and properties of pectinesterase from soursop (Anona muricata) pulp |
| title_full_unstemmed | Purification and properties of pectinesterase from soursop (Anona muricata) pulp |
| title_short | Purification and properties of pectinesterase from soursop (Anona muricata) pulp |
| title_sort | purification and properties of pectinesterase from soursop (anona muricata) pulp |
| url | http://psasir.upm.edu.my/id/eprint/51107/ http://psasir.upm.edu.my/id/eprint/51107/ http://psasir.upm.edu.my/id/eprint/51107/ http://psasir.upm.edu.my/id/eprint/51107/1/0000.pdf http://psasir.upm.edu.my/id/eprint/51107/7/1-s2.0-S030881469600043X-main.pdf |